(Linear) (Six-base) MAPSORT of: KIEE5064.seq Check: 4502 from: 1 to: 1662 KIEE5064.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. Acc65I G'GTAC_C Cuts at: 0 18 1662 Size: 18 1644 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 384 1139 1388 1662 Size: 279 105 755 249 274 Fragments arranged by size: 755 279 274 249 105 AflIII A'CryG_T Cuts at: 0 1430 1662 Size: 1430 232 AhdI GACnn_n'nnGTC Cuts at: 0 617 1662 Size: 617 1045 ApaLI G'TGCA_C Cuts at: 0 129 914 1662 Size: 129 785 748 Fragments arranged by size: 785 748 129 ApoI r'AATT_y Cuts at: 0 138 344 565 1662 Size: 138 206 221 1097 Fragments arranged by size: 1097 221 206 138 BanI G'GyrC_C Cuts at: 0 18 1662 Size: 18 1644 BbsI GAAGACnn'nnnn_ Cuts at: 0 644 1066 1662 Size: 644 422 596 Fragments arranged by size: 644 596 422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 994 1028 1662 Size: 994 34 634 Fragments arranged by size: 994 634 34 BfrBI ATG'CAT Cuts at: 0 553 1662 Size: 553 1109 Bme1580I G_kGCm'C Cuts at: 0 133 918 1662 Size: 133 785 744 Fragments arranged by size: 785 744 133 BmrI ACTGGGnnnn_n' Cuts at: 0 1496 1662 Size: 1496 166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1079 1662 Size: 1079 583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 806 1662 Size: 806 856 BsaAI yAC'GTr Cuts at: 0 1431 1662 Size: 1431 231 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 112 142 1494 1524 1662 Size: 112 30 1352 30 138 Fragments arranged by size: 1352 138 112 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 133 1496 1499 1662 Size: 133 1363 3 163 Fragments arranged by size: 1363 163 133 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1120 1662 Size: 1120 542 BsiHKAI G_wGCw'C Cuts at: 0 133 918 1350 1662 Size: 133 785 432 312 Fragments arranged by size: 785 432 312 133 Bsp1286I G_dGCh'C Cuts at: 0 133 918 1350 1662 Size: 133 785 432 312 Fragments arranged by size: 785 432 312 133 BstXI CCAn_nnnn'nTGG Cuts at: 0 525 1662 Size: 525 1137 BstYI r'GATC_y Cuts at: 0 1411 1534 1662 Size: 1411 123 128 Fragments arranged by size: 1411 128 123 Bsu36I CC'TnA_GG Cuts at: 0 1076 1662 Size: 1076 586 BtgI C'CryG_G Cuts at: 0 539 953 1662 Size: 539 414 709 Fragments arranged by size: 709 539 414 DrdI GACnn_nn'nnGTC Cuts at: 0 235 1662 Size: 235 1427 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 27 1662 Size: 27 1635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 384 1079 1139 1388 1662 Size: 279 105 695 60 249 274 Fragments arranged by size: 695 279 274 249 105 60 EcoO109I rG'GnC_Cy Cuts at: 0 205 619 1662 Size: 205 414 1043 Fragments arranged by size: 1043 414 205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1253 1662 Size: 1253 409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 603 635 804 836 1662 Size: 603 32 169 32 826 Fragments arranged by size: 826 603 169 32 32 HincII GTy'rAC Cuts at: 0 1020 1662 Size: 1020 642 HindIII A'AGCT_T Cuts at: 0 448 1662 Size: 448 1214 KpnI G_GTAC'C Cuts at: 0 22 1662 Size: 22 1640 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1389 1662 Size: 1389 273 MspA1I CmG'CkG Cuts at: 0 1090 1324 1662 Size: 1090 234 338 Fragments arranged by size: 1090 338 234 NcoI C'CATG_G Cuts at: 0 539 953 1662 Size: 539 414 709 Fragments arranged by size: 709 539 414 NsiI A_TGCA'T Cuts at: 0 555 1662 Size: 555 1107 NspI r_CATG'y Cuts at: 0 557 1662 Size: 557 1105 PflMI CCAn_nnn'nTGG Cuts at: 0 959 1531 1662 Size: 959 572 131 Fragments arranged by size: 959 572 131 PfoI T'CCnGG_A Cuts at: 0 152 192 1662 Size: 152 40 1470 Fragments arranged by size: 1470 152 40 PmlI CAC'GTG Cuts at: 0 1431 1662 Size: 1431 231 PpuMI rG'GwC_Cy Cuts at: 0 205 619 1662 Size: 205 414 1043 Fragments arranged by size: 1043 414 205 PstI C_TGCA'G Cuts at: 0 1133 1341 1451 1662 Size: 1133 208 110 211 Fragments arranged by size: 1133 211 208 110 PvuII CAG'CTG Cuts at: 0 1324 1662 Size: 1324 338 SfcI C'TryA_G Cuts at: 0 1129 1337 1447 1662 Size: 1129 208 110 215 Fragments arranged by size: 1129 215 208 110 SmlI C'TyrA_G Cuts at: 0 821 1662 Size: 821 841 SphI G_CATG'C Cuts at: 0 557 1662 Size: 557 1105 SspI AAT'ATT Cuts at: 0 1035 1662 Size: 1035 627 StyI C'CwwG_G Cuts at: 0 539 953 1662 Size: 539 414 709 Fragments arranged by size: 709 539 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1265 1662 Size: 1265 397 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 112 144 1662 Size: 112 32 1518 Fragments arranged by size: 1518 112 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 1038 1662 Size: 525 513 624 Fragments arranged by size: 624 525 513 XmnI GAAnn'nnTTC Cuts at: 0 851 1224 1401 1662 Size: 851 373 177 261 Fragments arranged by size: 851 373 261 177 Enzymes that do cut: Acc65I AcuI AflIII AhdI ApaLI ApoI BanI BbsI BcgI BfrBI Bme1580I BmrI BpmI BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI Bsp1286I BstXI BstYI Bsu36I BtgI DrdI EciI Eco57MI EcoO109I FalI Hin4I HincII HindIII KpnI MmeI MspA1I NcoI NsiI NspI PflMI PfoI PmlI PpuMI PstI PvuII SfcI SmlI SphI SspI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI