################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BcnI BfaI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MaeI MfeI MluI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 686 TCCGGA T^CCGGA AflIII 385 ACGTGT A^C[GA][CT]GT Asp700I 49 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 302 CTCGGG C^[CT]CG[GA]G BalI 425 TGGCCA TGG^CCA BbsI 949 GTCTTC GAAGAC(2/6) BclI 735 TGATCA T^GATCA BpmI 644 CTGGAG CTGGAG(16/14) BpuAI 949 GTCTTC GAAGAC(2/6) BsaWI 686 TCCGGA [AT]^CCGG[AT] BseAI 686 TCCGGA T^CCGGA BsgI 21 GTGCAG GTGCAG(16/14) BsmBI 64 GAGACG CGTCTC(1/5) BsoBI 302 CTCGGG C^[CT]CG[GA]G BspEI 686 TCCGGA T^CCGGA BsrDI 1095 CATTGC GCAATG(2/0) BstYI 306 GGATCT [GA]^GATC[CT] DraI 799 TTTAAA TTT^AAA DraII 923 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 425 TGGCCA [CT]^GGCC[GA] EarI 1075 GAAGAG CTCTTC(1/4) EcoO109I 923 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 496 GAATTC G^AATTC EcoT14I 862 CCAAGG C^C[AT][AT]GG EcoT22I 207 ATGCAT ATGCA^T FbaI 735 TGATCA T^GATCA HapII 687 CCGG C^CGG HincII 34 GTTGAC GT[CT]^[GA]AC HindII 34 GTTGAC GT[CT]^[GA]AC HpaII 687 CCGG C^CGG Ksp632I 1075 GAAGAG CTCTTC(1/4) MaeII 385 ACGT A^CGT MaeIII 520 GTGAC ^GT[ACGT]AC MflI 306 GGATCT [GA]^GATC[CT] MluNI 425 TGGCCA TGG^CCA MroI 686 TCCGGA T^CCGGA MscI 425 TGGCCA TGG^CCA MspI 687 CCGG C^CGG NsiI 207 ATGCAT ATGCA^T NspI 741 GCATGT [GA]CATG^[CT] PvuII 641 CAGCTG CAG^CTG StyI 862 CCAAGG C^C[AT][AT]GG Tsp45I 520 GTGAC ^GT[GC]AC XhoII 306 GGATCT [GA]^GATC[CT] XmnI 49 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 104 GGTACC G^GTACC 452 GGTACC AlwI 129 GGATC GGATC(4/5) 306 GGATC AseI 235 ATTAAT AT^TAAT 396 ATTAAT AsnI 235 ATTAAT AT^TAAT 396 ATTAAT Asp718I 104 GGTACC G^GTACC 452 GGTACC AvaII 554 GGACC G^G[AT]CC 943 GGACC BanI 104 GGTACC G^G[CT][GA]CC 452 GGTACC BbvI 729 GCAGC GCAGC(8/12) 189 GCTGC BciVI 661 GGATAC GTATCC(6/5) 1044 GTATCC BsmFI 825 GTCCC GGGAC(10/14) 931 GTCCC BspMI 857 ACCTGC ACCTGC(4/8) 1145 ACCTGC BstF5I 464 CATCC GGATG(2/0) 163 GGATG Fnu4HI 189 GCTGC GC^[ACGT]GC 729 GCAGC FokI 464 CATCC GGATG(9/13) 163 GGATG HindIII 777 AAGCTT A^AGCTT 840 AAGCTT ItaI 189 GCTGC GC^[ACGT]GC 729 GCAGC KpnI 104 GGTACC GGTAC^C 452 GGTACC MspA1I 100 CAGCGG C[AC]G^C[GT]G 641 CAGCTG MwoI 732 GCATGATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1132 GCAGTGGTGGC PshBI 235 ATTAAT AT^TAAT 396 ATTAAT PstI 1027 CTGCAG CTGCA^G 1130 CTGCAG TseI 189 GCTGC G^C[AT]GC 729 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AfaI AflII AluI ApoI BfrI BsaBI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HinfI HphI MamI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfcI SmlI TfiI Tru9I Tsp509I TspRI Van91I