(Circular) (Six-base) MAPSORT of: pFN21AE5060.seq Check: 8176 from: 1 to: 5884 pFN21AE5060 5884 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2871 2871 Size: 5884 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 2109 2457 3205 2109 Size: 348 748 4788 Fragments arranged by size: 4788 748 348 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 3947 5331 5599 1632 Size: 831 1484 1384 268 1917 Fragments arranged by size: 1917 1484 1384 831 268 AflII C'TTAA_G Cuts at: 820 1017 2188 2530 2848 820 Size: 197 1171 342 318 3856 Fragments arranged by size: 3856 1171 342 318 197 AflIII A'CryG_T Cuts at: 1203 2390 4783 5760 1203 Size: 1187 2393 977 1327 Fragments arranged by size: 2393 1327 1187 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2237 1184 Size: 1053 4831 AlwNI CAG_nnn'CTG Cuts at: 3627 5199 3627 Size: 1572 4312 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 2125 2317 2501 2549 3017 3193 3419 3571 Size: 632 192 184 48 468 176 226 152 Cuts at: 3571 5680 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 632 468 226 192 184 176 152 48 AseI AT'TA_AT Cuts at: 160 1032 2241 2402 4438 160 Size: 872 1209 161 2036 1606 Fragments arranged by size: 2036 1606 1209 872 161 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2307 3209 1870 Size: 73 364 902 4545 Fragments arranged by size: 4545 902 364 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2093 2126 2093 Size: 33 5851 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2457 3205 4660 618 Size: 325 217 627 322 348 748 1455 1842 Fragments arranged by size: 1842 1455 748 627 348 325 322 217 BanII G_rGCy'C Cuts at: 1391 3203 1391 Size: 1812 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2947 928 Size: 971 1048 3865 Fragments arranged by size: 3865 1048 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4075 4109 1903 Size: 34 2138 34 3678 Fragments arranged by size: 3678 2138 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2660 3060 3776 4986 2660 Size: 400 716 1210 3558 Fragments arranged by size: 3558 1210 716 400 BclI T'GATC_A Cuts at: 1548 2740 1548 Size: 1192 4692 BfrBI ATG'CAT Cuts at: 2214 2214 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5879 5879 Size: 5884 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 3945 5101 1790 Size: 2155 1156 2573 Fragments arranged by size: 2573 2155 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3615 4573 442 Size: 416 53 83 399 24 2198 958 1753 Fragments arranged by size: 2198 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2377 2379 2377 Size: 2 5882 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2670 4544 1239 Size: 1431 1874 2579 Fragments arranged by size: 2579 1874 1431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4009 4874 5172 4009 Size: 865 298 4721 Fragments arranged by size: 4721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4547 882 Size: 617 3048 2219 Fragments arranged by size: 3048 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3675 493 Size: 711 2471 2702 Fragments arranged by size: 2702 2471 711 BsaBI GATnn'nnATC Cuts at: 2480 2541 2684 5878 2480 Size: 61 143 3194 2486 Fragments arranged by size: 3194 2486 143 61 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3691 4073 4708 275 Size: 53 83 186 936 2158 382 635 1451 Fragments arranged by size: 2158 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2691 4317 4715 4989 5136 5547 2691 Size: 1626 398 274 147 411 3028 Fragments arranged by size: 3028 1626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5659 Fragments arranged by size: 5659 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5884 BseYI C'CCAG_C Cuts at: 2885 5087 5583 2885 Size: 2202 496 3186 Fragments arranged by size: 3186 2202 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2047 1163 Size: 573 311 5000 Fragments arranged by size: 5000 573 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3945 4030 5101 3945 Size: 85 1071 4728 Fragments arranged by size: 4728 1071 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 BsmBI CGTCTCn'nnnn_ Cuts at: 2063 2063 Size: 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3203 3945 4030 5101 1391 Size: 399 1413 742 85 1071 2174 Fragments arranged by size: 2174 1413 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2691 5547 2691 Size: 2856 3028 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2871 3159 3239 844 Size: 2027 288 80 3489 Fragments arranged by size: 3489 2027 288 80 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3099 4379 4553 65 Size: 1183 1851 1280 174 1396 Fragments arranged by size: 1851 1396 1280 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4528 4715 1700 Size: 110 2718 187 2869 Fragments arranged by size: 2869 2718 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5884 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstYI r'GATC_y Cuts at: 1972 2311 3214 3581 3966 3983 5435 5521 5533 Size: 339 903 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 903 385 367 346 339 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4393 Fragments arranged by size: 4393 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5145 Fragments arranged by size: 5145 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3144 3400 4194 4214 3130 Size: 14 256 794 20 4800 Fragments arranged by size: 4800 794 256 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 2806 3188 2806 Size: 382 5502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 3331 4220 Size: 54 1425 50 275 18 600 901 889 Cuts at: 4220 5849 8 Size: 1629 43 Fragments arranged by size: 1629 1425 901 889 600 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3075 3814 1353 Size: 1722 739 3423 Fragments arranged by size: 3423 1722 739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2887 4471 4845 4991 387 Size: 1064 138 1298 1584 374 146 1280 Fragments arranged by size: 1584 1298 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2670 3947 4544 5331 5599 1239 Size: 393 831 207 1277 597 787 268 1524 Fragments arranged by size: 1524 1277 831 787 597 393 268 207 EcoO109I rG'GnC_Cy Cuts at: 2929 2929 Size: 5884 EcoRI G'AATT_C Cuts at: 2501 3193 3571 2501 Size: 692 378 4814 Fragments arranged by size: 4814 692 378 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 5031 1678 Size: 3353 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4525 4557 4599 4631 1373 Size: 32 135 32 2953 32 42 32 2626 Fragments arranged by size: 2953 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 3228 3474 669 Size: 988 281 103 1187 246 3079 Fragments arranged by size: 3079 1187 988 281 246 103 HindIII A'AGCT_T Cuts at: 748 2782 2845 748 Size: 2034 63 3787 Fragments arranged by size: 3787 2034 63 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KpnI G_GTAC'C Cuts at: 2113 2461 3209 2113 Size: 348 748 4788 Fragments arranged by size: 4788 748 348 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 2644 2989 4998 5182 1305 Size: 904 435 345 2009 184 2007 Fragments arranged by size: 2009 2007 904 435 345 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2432 10 Size: 54 1425 343 600 3462 Fragments arranged by size: 3462 1425 600 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3842 4201 4360 518 Size: 648 2676 359 159 2042 Fragments arranged by size: 2676 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2648 3300 3977 5125 5370 1606 Size: 501 541 652 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 541 501 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4393 Fragments arranged by size: 4393 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NsiI A_TGCA'T Cuts at: 2216 2216 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2750 3242 4787 1132 Size: 97 1521 492 1545 2229 Fragments arranged by size: 2229 1545 1521 492 97 PasI CC'CwG_GG Cuts at: 1348 2064 1348 Size: 716 5168 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2648 2839 2945 1108 Size: 1540 191 106 4047 Fragments arranged by size: 4047 1540 191 106 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 2754 3454 2754 Size: 700 5184 PstI C_TGCA'G Cuts at: 830 3036 3139 3236 830 Size: 2206 103 97 3478 Fragments arranged by size: 3478 2206 103 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2648 2648 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2021 3032 3135 3232 4367 5048 5239 Size: 219 976 1011 103 97 1135 681 191 Cuts at: 5239 826 Size: 1471 Fragments arranged by size: 1471 1135 1011 976 681 219 191 103 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 3211 1872 Size: 1339 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2188 2530 2848 3988 4889 5151 Size: 197 926 245 342 318 1140 901 262 Cuts at: 5151 820 Size: 1553 Fragments arranged by size: 1553 1140 926 901 342 318 262 245 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5884 StyI C'CwwG_G Cuts at: 513 1065 2004 2867 3323 513 Size: 552 939 863 456 3074 Fragments arranged by size: 3074 939 863 552 456 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 3928 4081 4266 4711 325 Size: 1255 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5844 5876 5844 Size: 32 5852 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 3209 1870 Size: 1339 4545 XmnI GAAnn'nnTTC Cuts at: 2058 4013 2058 Size: 1955 3929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI