################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AgeI AhdI AluI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 77 TCCGGA T^CCGGA AcsI 499 GAATTC [GA]^AATT[CT] AcyI 289 GGCGCC G[GA]^CG[CT]C AfaI 277 GTAC GT^AC AflIII 94 ACGTGT A^C[GA][CT]GT AlwI 444 GATCC GGATC(4/5) AocI 450 CCTCAGG CC^T[ACGT]AGG ApoI 499 GAATTC [GA]^AATT[CT] AvaI 250 CCCGGG C^[CT]CG[GA]G BanI 289 GGCGCC G^G[CT][GA]CC BbeI 289 GGCGCC GGCGC^C BbrPI 93 CACGTG CAC^GTG BbvCI 157 GCTGAGG CCTCAGC(-5/-2) BcgI 498 CGAATTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 298 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 322 CTGGAG CTGGAG(16/14) BsaAI 93 CACGTG [CT]AC^GT[GA] BsaHI 289 GGCGCC G[GA]^CG[CT]C BsaWI 77 TCCGGA [AT]^CCGG[AT] BseAI 77 TCCGGA T^CCGGA BsmFI 339 GTCCC GGGAC(10/14) BsoBI 250 CCCGGG C^[CT]CG[GA]G BspEI 77 TCCGGA T^CCGGA BsrFI 53 GCCGGT [GA]^CCGG[CT] Bsu36I 450 CCTCAGG CC^T[ACGT]AGG CfoI 290 GCGC GCG^C Cfr10I 53 GCCGGT [GA]^CCGG[CT] Eco81I 450 CCTCAGG CC^T[ACGT]AGG EcoRI 499 GAATTC G^AATTC EcoT14I 293 CCTTGG C^C[AT][AT]GG HaeII 289 GGCGCC [GA]GCGC^[CT] HhaI 290 GCGC GCG^C Hin1I 289 GGCGCC G[GA]^CG[CT]C HinP1I 290 GCGC G^CGC HinfI 67 GAATC G^A[ACGT]TC KasI 289 GGCGCC G^GCGCC MaeII 94 ACGT A^CGT MaeIII 463 GTCAC ^GT[ACGT]AC MroI 77 TCCGGA T^CCGGA MspA1I 537 CAGCGG C[AC]G^C[GT]G NarI 289 GGCGCC GG^CGCC PmaCI 93 CACGTG CAC^GTG PmlI 93 CACGTG CAC^GTG PshAI 3 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 277 GTAC GT^AC SfaNI 557 GCATC GCATC(5/9) SfiI 297 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 289 GGCGCC GGC^GCC SmaI 250 CCCGGG CCC^GGG StuI 28 AGGCCT AGG^CCT StyI 293 CCTTGG C^C[AT][AT]GG TfiI 67 GAATC G^A[AT]TC Tsp45I 463 GTCAC ^GT[GC]AC Tsp509I 500 AATT ^AATT XmaCI 250 CCCGGG C^CCGGG XmaI 250 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BanII 175 GGGCTC G[GA]GC[CT]^C 427 GGGCTC BbvI 45 GCAGC GCAGC(8/12) 281 GCAGC BcnI 250 CCCGG CC^[GC]GG 251 CCGGG BsaI 227 GAGACC GGTCTC(1/5) 126 GGTCTC BsgI 189 CTGCAC GTGCAG(16/14) 16 GTGCAG BsmAI 227 GAGAC GTCTC(1/5) 127 GTCTC BsrI 146 ACTGG ACTGG(1/-1) 342 CCAGT DdeI 158 CTGAG C^T[ACGT]AG 451 CTCAG DpnII 216 GATC ^GATC 444 GATC DsaI 260 CCGTGG C^C[GA][CT]GG 299 CCGTGG EarI 169 CTCTTC CTCTTC(1/4) 520 CTCTTC EcoNI 153 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 231 CCTGGGCCAGG FauI 50 CCCGC CCCGC(4/6) 425 GCGGG HphI 479 TCACC GGTGA(8/7) 560 TCACC Ksp632I 169 CTCTTC CTCTTC(1/4) 520 CTCTTC MboI 216 GATC ^GATC 444 GATC MboII 170 TCTTC GAAGA(8/7) 521 TCTTC MslI 93 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 480 CACCCTAATG NciI 250 CCCGG CC^[GC]GG 251 CCGGG NdeII 216 GATC ^GATC 444 GATC Sau3AI 216 GATC ^GATC 444 GATC TseI 45 GCAGC G^C[AT]GC 281 GCAGC XcmI 311 CCATTTTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 389 CCATCCTCCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AvaII BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TspRI