(Linear) (Six-base) MAPSORT of: KIEE5057.seq Check: 8890 from: 1 to: 576 KIEE5057.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 576 Size: 207 369 AflIII A'CryG_T Cuts at: 0 94 576 Size: 94 482 AleI CACnn'nnGTG Cuts at: 0 97 576 Size: 97 479 ApoI r'AATT_y Cuts at: 0 499 576 Size: 499 77 AvaI C'yCGr_G Cuts at: 0 250 576 Size: 250 326 BanI G'GyrC_C Cuts at: 0 289 576 Size: 289 287 BanII G_rGCy'C Cuts at: 0 179 431 576 Size: 179 252 145 Fragments arranged by size: 252 179 145 BbeI G_GCGC'C Cuts at: 0 293 576 Size: 293 283 BbvCI CC'TCA_GC Cuts at: 0 158 576 Size: 158 418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 487 521 576 Size: 487 34 55 Fragments arranged by size: 487 55 34 BglI GCCn_nnn'nGGC Cuts at: 0 304 576 Size: 304 272 Bme1580I G_kGCm'C Cuts at: 0 146 251 576 Size: 146 105 325 Fragments arranged by size: 325 146 105 BmrI ACTGGGnnnn_n' Cuts at: 0 336 576 Size: 336 240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 576 Size: 343 233 Bpu10I CC'TnA_GC Cuts at: 0 158 576 Size: 158 418 BsaI GGTCTCn'nnnn_ Cuts at: 0 132 221 576 Size: 132 89 355 Fragments arranged by size: 355 132 89 BsaAI yAC'GTr Cuts at: 0 95 576 Size: 95 481 BsaHI Gr'CG_yC Cuts at: 0 290 576 Size: 290 286 BsaWI w'CCGG_w Cuts at: 0 77 576 Size: 77 499 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 408 439 508 576 Size: 385 23 31 69 68 Fragments arranged by size: 385 69 68 31 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 174 576 Size: 37 137 402 Fragments arranged by size: 402 137 37 Bsp1286I G_dGCh'C Cuts at: 0 146 179 251 431 576 Size: 146 33 72 180 145 Fragments arranged by size: 180 146 145 72 33 BspEI T'CCGG_A Cuts at: 0 77 576 Size: 77 499 BsrFI r'CCGG_y Cuts at: 0 53 576 Size: 53 523 Bsu36I CC'TnA_GG Cuts at: 0 451 576 Size: 451 125 BtgI C'CryG_G Cuts at: 0 260 299 576 Size: 260 39 277 Fragments arranged by size: 277 260 39 BtsI GCAGTG_nn' Cuts at: 0 503 576 Size: 503 73 EaeI y'GGCC_r Cuts at: 0 257 296 305 576 Size: 257 39 9 271 Fragments arranged by size: 271 257 39 9 EarI CTCTTCn'nnn_ Cuts at: 0 175 526 576 Size: 175 351 50 Fragments arranged by size: 351 175 50 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 343 576 Size: 207 136 233 Fragments arranged by size: 233 207 136 EcoNI CCTnn'n_nnAGG Cuts at: 0 157 235 576 Size: 157 78 341 Fragments arranged by size: 341 157 78 EcoRI G'AATT_C Cuts at: 0 499 576 Size: 499 77 HaeII r_GCGC'y Cuts at: 0 293 576 Size: 293 283 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 346 378 576 Size: 346 32 198 Fragments arranged by size: 346 198 32 KasI G'GCGC_C Cuts at: 0 289 576 Size: 289 287 MslI CAynn'nnrTG Cuts at: 0 97 484 576 Size: 97 387 92 Fragments arranged by size: 387 97 92 MspA1I CmG'CkG Cuts at: 0 539 576 Size: 539 37 NarI GG'CG_CC Cuts at: 0 290 576 Size: 290 286 PasI CC'CwG_GG Cuts at: 0 569 576 Size: 569 7 PmlI CAC'GTG Cuts at: 0 95 576 Size: 95 481 PshAI GACnn'nnGTC Cuts at: 0 7 576 Size: 7 569 SfiI GGCCn_nnn'nGGCC Cuts at: 0 304 576 Size: 304 272 SfoI GGC'GCC Cuts at: 0 291 576 Size: 291 285 SmaI CCC'GGG Cuts at: 0 252 576 Size: 252 324 SrfI GCCC'GGGC Cuts at: 0 252 576 Size: 252 324 StuI AGG'CCT Cuts at: 0 30 576 Size: 30 546 StyI C'CwwG_G Cuts at: 0 293 576 Size: 293 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 114 576 Size: 114 462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 396 576 Size: 318 78 180 Fragments arranged by size: 318 180 78 XmaI C'CCGG_G Cuts at: 0 250 576 Size: 250 326 Enzymes that do cut: AcuI AflIII AleI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BsgI Bsp1286I BspEI BsrFI Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoRI HaeII Hin4I KasI MslI MspA1I NarI PasI PmlI PshAI SfiI SfoI SmaI SrfI StuI StyI TaqII XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI