################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 6 GGCGCC G[GA]^CG[CT]C AlwI 24 GATCC GGATC(4/5) AlwNI 145 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 382 GGGCCC GGGCC^C AvaI 296 CCCGGG C^[CT]CG[GA]G BanI 6 GGCGCC G^G[CT][GA]CC BbeI 6 GGCGCC GGCGC^C BbvCI 364 GCTGAGG CCTCAGC(-5/-2) BfaI 163 CTAG C^TAG BsaHI 6 GGCGCC G[GA]^CG[CT]C BsoBI 296 CCCGGG C^[CT]CG[GA]G BstF5I 354 GGATG GGATG(2/0) BstYI 23 AGATCC [GA]^GATC[CT] DpnII 24 GATC ^GATC EarI 457 GAAGAG CTCTTC(1/4) EcoNI 360 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 182 CCAAGG C^C[AT][AT]GG FokI 354 GGATG GGATG(9/13) HgaI 91 GACGC GACGC(5/10) Hin1I 6 GGCGCC G[GA]^CG[CT]C KasI 6 GGCGCC G^GCGCC Ksp632I 457 GAAGAG CTCTTC(1/4) MaeI 163 CTAG C^TAG MaeIII 398 GTGAC ^GT[ACGT]AC MboI 24 GATC ^GATC MboII 457 GAAGA GAAGA(8/7) MflI 23 AGATCC [GA]^GATC[CT] NarI 6 GGCGCC GG^CGCC NdeII 24 GATC ^GATC SacI 274 GAGCTC GAGCT^C Sau3AI 24 GATC ^GATC SbfI 246 CCTGCAGG CCTGCA^GG SfaNI 355 GATGC GCATC(5/9) SfoI 6 GGCGCC GGC^GCC SgrAI 58 CACCGGCG C[GA]^CCGG[CT]G SmaI 296 CCCGGG CCC^GGG Sse8387I 246 CCTGCAGG CCTGCA^GG StyI 182 CCAAGG C^C[AT][AT]GG Tsp45I 398 GTGAC ^GT[GC]AC XhoII 23 AGATCC [GA]^GATC[CT] XmaCI 296 CCCGGG C^CCGGG XmaI 296 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BanII 274 GAGCTC G[GA]GC[CT]^C 382 GGGCCC BlpI 301 GCTGAGC GC^T[ACGT]AGC 370 GCTGAGC Bpu1102I 301 GCTGAGC GC^T[ACGT]AGC 370 GCTGAGC BssHII 63 GCGCGC G^CGCGC 153 GCGCGC CelII 301 GCTGAGC GC^T[ACGT]AGC 370 GCTGAGC DraII 381 GGGGCCC [GA]G^G[ACGT]CC[CT] 382 GGGCCCC EcoO109I 381 GGGGCCC [GA]G^G[ACGT]CC[CT] 382 GGGCCCC PvuII 148 CAGCTG CAG^CTG 214 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AluI Alw44I ApaLI AspHI AvaII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EaeI EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV PstI Sau96I ScrFI SfcI TseI TspRI