(Circular) (Six-base) MAPSORT of: pFN21AE5053.seq Check: 4599 from: 1 to: 5212 pFN21AE5053 5212 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3022 278 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2555 1937 Size: 618 4594 Acc65I G'GTAC_C Cuts at: 2533 2533 Size: 5212 AclI AA'CG_TT Cuts at: 1563 3339 3712 1563 Size: 1776 373 3063 Fragments arranged by size: 3063 1776 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3275 4659 4927 1632 Size: 1643 1384 268 1917 Fragments arranged by size: 1917 1643 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5015 AflIII A'CryG_T Cuts at: 1203 4111 5088 1203 Size: 2908 977 1327 Fragments arranged by size: 2908 1327 977 AgeI A'CCGG_T Cuts at: 4043 4043 Size: 5212 AhdI GACnn_n'nnGTC Cuts at: 3941 3941 Size: 5212 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5212 AlwNI CAG_nnn'CTG Cuts at: 2155 2955 4527 2155 Size: 800 1572 2840 Fragments arranged by size: 2840 1572 800 ApaI G_GGCC'C Cuts at: 2391 2391 Size: 5212 ApaLI G'TGCA_C Cuts at: 2060 2114 2264 3269 4425 2060 Size: 54 150 1005 1156 2847 Fragments arranged by size: 2847 1156 1005 150 54 ApoI r'AATT_y Cuts at: 1493 2521 2747 2899 5008 1493 Size: 1028 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 226 152 AseI AT'TA_AT Cuts at: 160 1032 3766 160 Size: 872 2734 1606 Fragments arranged by size: 2734 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 AvaI C'yCGr_G Cuts at: 1870 1943 2301 2537 1870 Size: 73 358 236 4545 Fragments arranged by size: 4545 358 236 73 BamHI G'GATC_C Cuts at: 2542 2909 2542 Size: 367 4845 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2533 3988 618 Size: 325 217 627 224 522 1455 1842 Fragments arranged by size: 1842 1455 627 522 325 224 217 BanII G_rGCy'C Cuts at: 1391 2283 2391 2531 1391 Size: 892 108 140 4072 Fragments arranged by size: 4072 892 140 108 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 5212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4241 BbvCI CC'TCA_GC Cuts at: 2370 2370 Size: 5212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3403 3437 1903 Size: 34 1466 34 3678 Fragments arranged by size: 3678 1466 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3104 4314 3104 Size: 1210 4002 BclI T'GATC_A Cuts at: 1548 1548 Size: 5212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3823 136 Size: 107 122 71 631 605 337 1814 1525 Fragments arranged by size: 1814 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5207 5207 Size: 5212 BlpI GC'TnA_GC Cuts at: 2307 2376 2629 2307 Size: 69 253 4890 Fragments arranged by size: 4890 253 69 Bme1580I G_kGCm'C Cuts at: 1790 2064 2118 2268 2391 3273 4429 1790 Size: 274 54 150 123 882 1156 2573 Fragments arranged by size: 2573 1156 882 274 150 123 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2186 2466 2943 Size: 416 53 83 399 24 769 280 477 Cuts at: 2943 3901 442 Size: 958 1753 Fragments arranged by size: 1753 958 769 477 416 399 280 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5212 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2195 2468 3872 1239 Size: 863 93 273 1404 2579 Fragments arranged by size: 2579 1404 863 273 93 Bpu10I CC'TnA_GC Cuts at: 2370 2370 Size: 5212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3337 4202 4500 3337 Size: 865 298 4049 Fragments arranged by size: 4049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3875 882 Size: 617 2376 2219 Fragments arranged by size: 2376 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3003 493 Size: 711 1799 2702 Fragments arranged by size: 2702 1799 711 BsaBI GATnn'nnATC Cuts at: 5206 5206 Size: 5212 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3019 3401 4036 Size: 53 83 186 936 479 1007 382 635 Cuts at: 4036 275 Size: 1451 Fragments arranged by size: 1451 1007 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3645 4043 4317 4464 4875 3645 Size: 398 274 147 411 3982 Fragments arranged by size: 3982 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4987 Fragments arranged by size: 4987 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5212 BseYI C'CCAG_C Cuts at: 2348 4415 4911 2348 Size: 2067 496 2649 Fragments arranged by size: 2649 2067 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4639 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2662 3423 4451 1540 Size: 275 186 661 761 1028 2301 Fragments arranged by size: 2301 1028 761 661 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2118 2268 2283 3273 3358 4429 2064 Size: 54 150 15 990 85 1071 2847 Fragments arranged by size: 2847 1071 990 150 85 54 15 BsmI GAATG_Cn' Cuts at: 2723 2816 2723 Size: 93 5119 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2118 2268 2283 2391 2531 3273 Size: 399 274 54 150 15 108 140 742 Cuts at: 3273 3358 4429 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 274 150 140 108 85 54 15 BspEI T'CCGG_A Cuts at: 4875 4875 Size: 5212 BspHI T'CATG_A Cuts at: 3101 4831 3101 Size: 1730 3482 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2567 844 Size: 1723 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3099 1394 Size: 429 1276 3507 Fragments arranged by size: 3507 1276 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3707 3881 65 Size: 1183 2459 174 1396 Fragments arranged by size: 2459 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2064 2334 2395 3856 4043 1700 Size: 110 207 47 270 61 1461 187 2869 Fragments arranged by size: 2869 1461 270 207 187 110 61 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5212 BssHII G'CGCG_C Cuts at: 1925 2068 2158 1925 Size: 143 90 4979 Fragments arranged by size: 4979 143 90 BssSI C'ACGA_G Cuts at: 3272 4284 3272 Size: 1012 4200 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5212 BstBI TT'CG_AA Cuts at: 4016 4016 Size: 5212 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5212 BstYI r'GATC_y Cuts at: 1972 2028 2542 2909 3294 3311 4763 4849 4861 Size: 56 514 367 385 17 1452 86 12 Cuts at: 4861 5207 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 514 385 367 346 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4660 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4473 Fragments arranged by size: 4473 683 56 BtsI GCAGTG_nn' Cuts at: 2499 2728 3522 3542 2499 Size: 229 794 20 4169 Fragments arranged by size: 4169 794 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5177 DraI TTT'AAA Cuts at: 2516 2516 Size: 5212 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4219 809 Size: 507 2903 1802 Fragments arranged by size: 2903 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2336 2397 2410 Size: 54 1425 50 275 18 506 61 13 Cuts at: 2410 2659 3548 5177 8 Size: 249 889 1629 43 Fragments arranged by size: 1629 1425 889 506 275 249 61 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2659 1537 Size: 275 847 4090 Fragments arranged by size: 4090 847 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 3142 1353 Size: 1104 685 3423 Fragments arranged by size: 3423 1104 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2483 3799 4173 4319 387 Size: 1064 138 528 366 1316 374 146 1280 Fragments arranged by size: 1316 1280 1064 528 374 366 146 138 EcoICRI GAG'CTC Cuts at: 2281 2281 Size: 5212 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2195 2468 3275 3872 4659 4927 Size: 393 470 93 273 807 597 787 268 Cuts at: 4927 1239 Size: 1524 Fragments arranged by size: 1524 807 787 597 470 393 273 268 93 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 5212 EcoO109I rG'GnC_Cy Cuts at: 2387 2388 2387 Size: 1 5211 EcoRI G'AATT_C Cuts at: 2521 2899 2521 Size: 378 4834 FspI TGC'GCA Cuts at: 3718 3718 Size: 5212 HaeII r_GCGC'y Cuts at: 1678 2015 2134 2383 4359 1678 Size: 337 119 249 1976 2531 Fragments arranged by size: 2531 1976 337 249 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3853 3885 3927 3959 1373 Size: 32 135 32 2281 32 42 32 2626 Fragments arranged by size: 2626 2281 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2556 2802 669 Size: 988 281 618 246 3079 Fragments arranged by size: 3079 988 618 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5212 HpaI GTT'AAC Cuts at: 2802 2802 Size: 5212 KasI G'GCGC_C Cuts at: 2011 2011 Size: 5212 KpnI G_GTAC'C Cuts at: 2537 2537 Size: 5212 MfeI C'AATT_G Cuts at: 2811 2811 Size: 5212 MluI A'CGCG_T Cuts at: 5088 5088 Size: 5212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4326 4510 1305 Size: 3021 184 2007 Fragments arranged by size: 3021 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3390 Fragments arranged by size: 3390 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3529 3688 518 Size: 648 2004 359 159 2042 Fragments arranged by size: 2042 2004 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 2155 2221 2628 3305 4453 4698 1606 Size: 469 80 66 407 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 469 407 245 80 66 NaeI GCC'GGC Cuts at: 2019 2336 2397 2019 Size: 317 61 4834 Fragments arranged by size: 4834 317 61 NarI GG'CG_CC Cuts at: 2012 2012 Size: 5212 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4660 NdeI CA'TA_TG Cuts at: 387 387 Size: 5212 NgoMIV G'CCGG_C Cuts at: 2017 2334 2395 2017 Size: 317 61 4834 Fragments arranged by size: 4834 317 61 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5212 NotI GC'GGCC_GC Cuts at: 2659 2659 Size: 5212 NspI r_CATG'y Cuts at: 1132 1229 2570 4115 1132 Size: 97 1341 1545 2229 Fragments arranged by size: 2229 1545 1341 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5212 PciI A'CATG_T Cuts at: 4111 4111 Size: 5212 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5212 PfoI T'CCnGG_A Cuts at: 2031 2211 2031 Size: 180 5032 PmeI GTTT'AAAC Cuts at: 2516 2516 Size: 5212 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3281 3313 4794 4826 3281 Size: 32 1481 32 3667 Fragments arranged by size: 3667 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5212 PsiI TTA'TAA Cuts at: 2782 2782 Size: 5212 PspOMI G'GGCC_C Cuts at: 2387 2387 Size: 5212 PstI C_TGCA'G Cuts at: 830 2028 2256 2319 2494 2564 830 Size: 1198 228 63 175 70 3478 Fragments arranged by size: 3478 1198 228 175 70 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5212 PvuII CAG'CTG Cuts at: 2155 2221 2155 Size: 66 5146 SacI G_AGCT'C Cuts at: 2283 2283 Size: 5212 SalI G'TCGA_C Cuts at: 1936 2554 1936 Size: 618 4594 SbfI CC_TGCA'GG Cuts at: 2256 2564 2256 Size: 308 4904 ScaI AGT'ACT Cuts at: 3460 5172 3460 Size: 1712 3500 SfcI C'TryA_G Cuts at: 826 1045 2024 2252 2315 2490 2560 3695 4376 Size: 219 979 228 63 175 70 1135 681 Cuts at: 4376 4567 826 Size: 191 1471 Fragments arranged by size: 1471 1135 979 681 228 219 191 175 70 63 SfoI GGC'GCC Cuts at: 2013 2013 Size: 5212 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 SgrAI Cr'CCGG_yG Cuts at: 1700 2064 1700 Size: 364 4848 SmaI CCC'GGG Cuts at: 1872 2303 2539 1872 Size: 431 236 4545 Fragments arranged by size: 4545 431 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 3316 4217 4479 820 Size: 197 926 1373 901 262 1553 Fragments arranged by size: 1553 1373 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3003 493 Size: 2510 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5212 SphI G_CATG'C Cuts at: 1132 2570 1132 Size: 1438 3774 SspI AAT'ATT Cuts at: 5 52 3136 5 Size: 47 3084 2081 Fragments arranged by size: 3084 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5212 StyI C'CwwG_G Cuts at: 513 1065 2187 2651 513 Size: 552 1122 464 3074 Fragments arranged by size: 3074 1122 552 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2430 3239 3256 3409 3594 4039 325 Size: 1255 850 809 17 153 185 445 1498 Fragments arranged by size: 1498 1255 850 809 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3458 5170 96 Size: 274 80 33 51 1443 1481 1712 138 Fragments arranged by size: 1712 1481 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5172 5204 5172 Size: 32 5180 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5212 XbaI T'CTAG_A Cuts at: 2548 2548 Size: 5212 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5212 XmaI C'CCGG_G Cuts at: 1870 2301 2537 1870 Size: 431 236 4545 Fragments arranged by size: 4545 431 236 XmnI GAAnn'nnTTC Cuts at: 3341 3341 Size: 5212 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3020 276 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI