################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI AlwNI AocI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfuI SnaBI SpeI SphI Sse8387I SspBI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1100 GGTACC G^GTACC AgeI 504 ACCGGT A^CCGGT Alw44I 967 GTGCAC G^TGCAC Aor51HI 73 AGCGCT AGC^GCT ApaLI 967 GTGCAC G^TGCAC Asp718I 1100 GGTACC G^GTACC AviII 869 TGCGCA TGC^GCA BalI 557 TGGCCA TGG^CCA BbrPI 427 CACGTG CAC^GTG BbsI 97 GAAGAC GAAGAC(2/6) BpmI 1345 CTCCAG CTGGAG(16/14) BpuAI 97 GAAGAC GAAGAC(2/6) BsaAI 427 CACGTG [CT]AC^GT[GA] BsaWI 504 ACCGGT [AT]^CCGG[AT] BsgI 1478 GTGCAG GTGCAG(16/14) BsmBI 853 GAGACG CGTCTC(1/5) BspDI 937 ATCGAT AT^CGAT BstAPI 1406 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 669 GGTGACC G^GT[ACGT]ACC BstPI 669 GGTGACC G^GT[ACGT]ACC BstYI 881 AGATCC [GA]^GATC[CT] ClaI 937 ATCGAT AT^CGAT DraIII 396 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 73 AGCGCT AGC^GCT EcoO65I 669 GGTGACC G^GT[ACGT]ACC EcoT14I 1247 CCAAGG C^C[AT][AT]GG FspI 869 TGCGCA TGC^GCA KpnI 1100 GGTACC GGTAC^C MflI 881 AGATCC [GA]^GATC[CT] MluNI 557 TGGCCA TGG^CCA MscI 557 TGGCCA TGG^CCA MseI 464 TTAA T^TAA PaeR7I 886 CTCGAG C^TCGAG PinAI 504 ACCGGT A^CCGGT PmaCI 427 CACGTG CAC^GTG PmlI 427 CACGTG CAC^GTG PpuMI 1299 GGGACCC [GA]G^G[AT]CC[CT] PvuII 115 CAGCTG CAG^CTG SapI 1209 GCTCTTC GCTCTTC(1/4) SfiI 468 GGCCTTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 503 CACCGGTG C[GA]^CCGG[CT]G SspI 1551 AATATT AAT^ATT StuI 467 AGGCCT AGG^CCT StyI 1247 CCAAGG C^C[AT][AT]GG Tru9I 464 TTAA T^TAA XhoI 886 CTCGAG C^TCGAG XhoII 881 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 606 GATCC GGATC(4/5) 882 GATCC ApaI 717 GGGCCC GGGCC^C 1261 GGGCCC BcgI 1425 CGAAGTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 878 GCAAGATCCTCG BsiEI 218 CGGCCG CG[GA][CT]^CG 266 CGGCCG BsmAI 853 GAGAC GTCTC(1/5) 1156 GAGAC BsrBI 1278 CCGCTC CCGCTC(-3/-3) 1342 CCGCTC BsrI 874 ACTGG ACTGG(1/-1) 523 CCAGT BssHII 105 GCGCGC G^CGCGC 838 GCGCGC DdeI 77 CTAAG C^T[ACGT]AG 582 CTCAG EagI 218 CGGCCG C^GGCCG 266 CGGCCG EarI 1210 CTCTTC CTCTTC(1/4) 1239 CTCTTC EclXI 218 CGGCCG C^GGCCG 266 CGGCCG Eco52I 218 CGGCCG C^GGCCG 266 CGGCCG HinfI 1042 GACTC G^A[ACGT]TC 1316 GACTC Ksp632I 1210 CTCTTC CTCTTC(1/4) 1239 CTCTTC KspI 254 CCGCGG CCGC^GG 1295 CCGCGG NaeI 216 GCCGGC GCC^GGC 234 GCCGGC NgoMIV 216 GCCGGC G^CCGGC 234 GCCGGC PleI 1042 GACTC GAGTC(4/5) 1316 GACTC SacII 254 CCGCGG CCGC^GG 1295 CCGCGG SmaI 1266 CCCGGG CCC^GGG 1332 CCCGGG SmlI 471 CTTGAG C^T[CT][GA]AG 886 CTCGAG TspRI 524 CAGTG CA[GC]TG[ACGT][ACGT]^ 873 CACTG XmaCI 1266 CCCGGG C^CCGGG 1332 CCCGGG XmaI 1266 CCCGGG C^CCGGG 1332 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TseI Tsp45I TthHB8I