(Circular) (Six-base) MAPSORT of: pFN21AE4127.seq Check: 9600 from: 1 to: 6265 pFN21AE4127 6265 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3105 3586 3105 Size: 481 5784 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2402 4328 5712 5980 1632 Size: 470 300 1926 1384 268 1917 Fragments arranged by size: 1926 1917 1384 470 300 268 AfeI AGC'GCT Cuts at: 2080 2080 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 2509 5096 2509 Size: 2587 3678 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 3209 1166 Size: 2043 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 2516 Size: 32 6233 AlwNI CAG_nnn'CTG Cuts at: 4008 5580 4008 Size: 1572 4693 ApaI G_GGCC'C Cuts at: 2726 3270 2726 Size: 544 5721 ApaLI G'TGCA_C Cuts at: 2972 4322 5478 2972 Size: 1350 1156 3759 Fragments arranged by size: 3759 1350 1156 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2891 3271 3308 3325 3337 3590 1870 Size: 73 948 380 37 17 12 253 4545 Fragments arranged by size: 4545 948 380 253 73 37 17 12 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2263 2636 2692 2819 Size: 325 217 627 420 56 373 56 127 Cuts at: 2819 3105 3330 3586 5041 618 Size: 286 225 256 1455 1842 Fragments arranged by size: 1842 1455 627 420 373 325 286 256 225 217 127 56 56 BanII G_rGCy'C Cuts at: 1391 2163 2726 2930 3270 3392 3584 1391 Size: 772 563 204 340 122 192 4072 Fragments arranged by size: 4072 772 563 340 204 192 122 BbeI G_GCGC'C Cuts at: 2211 2267 2640 3334 2211 Size: 56 373 694 5142 Fragments arranged by size: 5142 694 373 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 928 Size: 971 210 5084 Fragments arranged by size: 5084 971 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2872 2906 3419 3453 4456 Size: 34 50 34 851 34 513 34 1003 Cuts at: 4456 4490 1903 Size: 34 3678 Fragments arranged by size: 3678 1003 851 513 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 2282 2385 Size: 107 122 71 631 605 502 108 103 Cuts at: 2385 2480 3396 4876 136 Size: 95 916 1480 1525 Fragments arranged by size: 1525 1480 916 631 605 502 122 108 107 103 95 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2421 2697 2726 2824 2976 3270 3453 4326 Size: 631 276 29 98 152 294 183 873 Cuts at: 4326 5482 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 873 631 294 276 183 152 98 29 BmgBI CAC'GTC Cuts at: 1318 2560 1318 Size: 1242 5023 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 3365 2620 Size: 745 5520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3335 4925 1239 Size: 2096 1590 2579 Fragments arranged by size: 2579 2096 1590 Bpu10I CC'TnA_GC Cuts at: 2082 2082 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2497 4390 5255 5553 2497 Size: 1893 865 298 3209 Fragments arranged by size: 3209 1893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2434 4056 493 Size: 711 1230 1622 2702 Fragments arranged by size: 2702 1622 1230 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2264 2303 2340 Size: 53 83 186 936 675 56 39 37 Cuts at: 2340 2637 2961 3331 4072 4454 5089 275 Size: 297 324 370 741 382 635 1451 Fragments arranged by size: 1451 936 741 675 635 382 370 324 297 186 83 56 53 39 37 BsaWI w'CCGG_w Cuts at: 2509 4698 5096 5370 5517 5928 2509 Size: 2189 398 274 147 411 2846 Fragments arranged by size: 2846 2189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 1178 Size: 30 165 30 724 30 5286 Fragments arranged by size: 5286 724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2864 3211 3220 3223 3226 3229 3232 1186 Size: 1678 347 9 3 3 3 3 4219 Fragments arranged by size: 4219 1678 347 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2307 3263 5468 5964 2307 Size: 956 2205 496 2608 Fragments arranged by size: 2608 2205 956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3504 1163 Size: 573 1768 3924 Fragments arranged by size: 3924 1768 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2226 2274 3715 4476 5504 1540 Size: 275 186 225 48 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 225 186 48 BsiHKAI G_wGCw'C Cuts at: 2433 2601 2976 3216 4326 4411 5482 2433 Size: 168 375 240 1110 85 1071 3216 Fragments arranged by size: 3216 1110 1071 375 240 168 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 BsmBI CGTCTCn'nnnn_ Cuts at: 2852 2852 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2421 2433 2601 2697 2726 2824 Size: 399 373 258 12 168 96 29 98 Cuts at: 2824 2930 2976 3216 3270 3392 3453 3584 4326 Size: 106 46 240 54 122 61 131 742 Cuts at: 4326 4411 5482 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 258 240 168 131 122 106 98 96 85 61 54 46 29 12 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3285 3349 4152 1394 Size: 429 1462 64 803 3507 Fragments arranged by size: 3507 1462 803 429 64 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2221 2239 2509 4909 5096 1700 Size: 110 411 18 270 2400 187 2869 Fragments arranged by size: 2869 2400 411 270 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 2110 2843 1925 Size: 185 733 5347 Fragments arranged by size: 5347 733 185 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 3417 794 Size: 2623 3642 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 2674 1184 Size: 1490 4775 BstYI r'GATC_y Cuts at: 1972 2886 3595 3962 4347 4364 5816 5902 5914 Size: 914 709 367 385 17 1452 86 12 Cuts at: 5914 6260 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 914 709 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2172 2259 2400 3300 513 Size: 552 1107 87 141 900 3478 Fragments arranged by size: 3478 1107 900 552 141 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2851 487 Size: 683 56 1625 3901 Fragments arranged by size: 3901 1625 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 2943 1554 Size: 1389 4876 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 2406 2406 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2271 2436 Size: 54 1425 50 275 18 393 48 165 Cuts at: 2436 2562 2668 3156 3315 3712 4601 6230 8 Size: 126 106 488 159 397 889 1629 43 Fragments arranged by size: 1629 1425 889 488 397 393 275 165 159 126 106 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2223 2271 3712 1537 Size: 275 411 48 1441 4090 Fragments arranged by size: 4090 1441 411 275 48 EarI CTCTTCn'nnn_ Cuts at: 1353 3221 3250 4195 1353 Size: 1868 29 945 3423 Fragments arranged by size: 3423 1868 945 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3238 4852 5226 5372 387 Size: 1064 138 1649 1614 374 146 1280 Fragments arranged by size: 1649 1614 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2402 3335 4328 4925 5712 5980 Size: 393 470 300 933 993 597 787 268 Cuts at: 5980 1239 Size: 1524 Fragments arranged by size: 1524 993 933 787 597 470 393 300 268 EcoO109I rG'GnC_Cy Cuts at: 2723 3061 3267 3305 2723 Size: 338 206 38 5683 Fragments arranged by size: 5683 338 206 38 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 2876 4771 2876 Size: 1895 4370 FspAI rTGC'GCAy Cuts at: 2876 2876 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2082 2211 2267 2640 3334 5412 1678 Size: 404 129 56 373 694 2078 2531 Fragments arranged by size: 2531 2078 694 404 373 129 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KasI G'GCGC_C Cuts at: 2207 2263 2636 3330 2207 Size: 56 373 694 5142 Fragments arranged by size: 5142 694 373 56 KpnI G_GTAC'C Cuts at: 3109 3590 3109 Size: 481 5784 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2982 5379 5563 1305 Size: 1677 2397 184 2007 Fragments arranged by size: 2397 2007 1677 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3711 Fragments arranged by size: 3711 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 2870 3209 4223 4582 4741 518 Size: 648 1518 186 339 1014 359 159 2042 Fragments arranged by size: 2042 1518 1014 648 359 339 186 159 MspA1I CmG'CkG Cuts at: 1606 2122 2261 2594 3175 3302 3681 4358 5506 Size: 516 139 333 581 127 379 677 1148 Cuts at: 5506 5751 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 581 516 379 333 245 139 127 NaeI GCC'GGC Cuts at: 2223 2241 2223 Size: 18 6247 NarI GG'CG_CC Cuts at: 2208 2264 2637 3331 2208 Size: 56 373 694 5142 Fragments arranged by size: 5142 694 373 56 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5713 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2221 2239 2221 Size: 18 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 2434 2434 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3305 3305 Size: 6265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2722 3266 2722 Size: 544 5721 PspXI vC'TCGA_Gb Cuts at: 2891 2891 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2122 2122 Size: 6265 SacII CC_GC'GG Cuts at: 2262 3303 2262 Size: 1041 5224 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 3305 3305 Size: 6265 SapI GCTCTTCn'nnn_ Cuts at: 3221 3221 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 3613 4748 5429 5620 826 Size: 219 2568 1135 681 191 1471 Fragments arranged by size: 2568 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2480 2480 Size: 6265 SfoI GGC'GCC Cuts at: 2209 2265 2638 3332 2209 Size: 56 373 694 5142 Fragments arranged by size: 5142 694 373 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 2509 1700 Size: 809 5456 SmaI CCC'GGG Cuts at: 1872 3273 3339 3592 1872 Size: 1401 66 253 4545 Fragments arranged by size: 4545 1401 253 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2476 2891 4369 5270 5532 820 Size: 197 926 533 415 1478 901 262 1553 Fragments arranged by size: 1553 1478 926 901 533 415 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 3558 4189 5 Size: 47 3506 631 2081 Fragments arranged by size: 3506 2081 631 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 5308 StyI C'CwwG_G Cuts at: 513 1065 3252 3704 513 Size: 552 2187 452 3074 Fragments arranged by size: 3074 2187 552 452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2103 3062 4292 4309 4462 4647 5092 Size: 1255 523 959 1230 17 153 185 445 Cuts at: 5092 325 Size: 1498 Fragments arranged by size: 1498 1255 1230 959 523 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 3144 4511 Size: 274 80 33 51 1443 918 249 1367 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1367 918 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3500 3532 6225 6257 3500 Size: 32 2693 32 3508 Fragments arranged by size: 3508 2693 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 2891 1943 Size: 948 5317 XmaI C'CCGG_G Cuts at: 1870 3271 3337 3590 1870 Size: 1401 66 253 4545 Fragments arranged by size: 4545 1401 253 66 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PsrI RsrII SacI SexAI SrfI SwaI XcmI