(Linear) (Six-base) MAPSORT of: KIEE4127.seq Check: 4209 from: 1 to: 1560 KIEE4127.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. Acc65I G'GTAC_C Cuts at: 0 1100 1560 Size: 1100 460 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 397 1560 Size: 97 300 1163 Fragments arranged by size: 1163 300 97 AfeI AGC'GCT Cuts at: 0 75 1560 Size: 75 1485 AgeI A'CCGG_T Cuts at: 0 504 1560 Size: 504 1056 AleI CACnn'nnGTG Cuts at: 0 1204 1560 Size: 1204 356 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 511 543 1560 Size: 511 32 1017 Fragments arranged by size: 1017 511 32 ApaI G_GGCC'C Cuts at: 0 721 1265 1560 Size: 721 544 295 Fragments arranged by size: 721 544 295 ApaLI G'TGCA_C Cuts at: 0 967 1560 Size: 967 593 AvaI C'yCGr_G Cuts at: 0 886 1266 1303 1320 1332 1560 Size: 886 380 37 17 12 228 Fragments arranged by size: 886 380 228 37 17 12 BanI G'GyrC_C Cuts at: 0 202 258 631 687 814 1100 1325 1560 Size: 202 56 373 56 127 286 225 235 Fragments arranged by size: 373 286 235 225 202 127 56 56 BanII G_rGCy'C Cuts at: 0 158 721 925 1265 1387 1560 Size: 158 563 204 340 122 173 Fragments arranged by size: 563 340 204 173 158 122 BbeI G_GCGC'C Cuts at: 0 206 262 635 1329 1560 Size: 206 56 373 694 231 Fragments arranged by size: 694 373 231 206 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 104 1560 Size: 104 1456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 867 901 1414 1448 1560 Size: 867 34 513 34 112 Fragments arranged by size: 867 513 112 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 169 277 380 475 1391 1560 Size: 169 108 103 95 916 169 Fragments arranged by size: 916 169 169 108 103 95 Bme1580I G_kGCm'C Cuts at: 0 416 692 721 819 971 1265 1448 1560 Size: 416 276 29 98 152 294 183 112 Fragments arranged by size: 416 294 276 183 152 112 98 29 BmgBI CAC'GTC Cuts at: 0 555 1560 Size: 555 1005 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 615 1360 1560 Size: 615 745 200 Fragments arranged by size: 745 615 200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1330 1560 Size: 1330 230 Bpu10I CC'TnA_GC Cuts at: 0 77 1560 Size: 77 1483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 492 1560 Size: 492 1068 BsaAI yAC'GTr Cuts at: 0 429 1560 Size: 429 1131 BsaHI Gr'CG_yC Cuts at: 0 203 259 298 335 632 956 1326 1560 Size: 203 56 39 37 297 324 370 234 Fragments arranged by size: 370 324 297 234 203 56 39 37 BsaWI w'CCGG_w Cuts at: 0 504 1560 Size: 504 1056 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 122 152 1560 Size: 122 30 1408 Fragments arranged by size: 1408 122 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 859 1206 1215 1218 1221 1224 1227 1560 Size: 859 347 9 3 3 3 3 333 Fragments arranged by size: 859 347 333 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 302 1258 1560 Size: 302 956 302 Fragments arranged by size: 956 302 302 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1499 1560 Size: 1499 61 BsiEI CG_ry'CG Cuts at: 0 221 269 1560 Size: 221 48 1291 Fragments arranged by size: 1291 221 48 BsiHKAI G_wGCw'C Cuts at: 0 428 596 971 1211 1560 Size: 428 168 375 240 349 Fragments arranged by size: 428 375 349 240 168 BsmBI CGTCTCn'nnnn_ Cuts at: 0 847 1560 Size: 847 713 Bsp1286I G_dGCh'C Cuts at: 0 158 416 428 596 692 721 819 925 Size: 158 258 12 168 96 29 98 106 Cuts at: 925 971 1211 1265 1387 1448 1560 Size: 46 240 54 122 61 112 Fragments arranged by size: 258 240 168 158 122 112 106 98 96 61 54 46 29 12 BsrBI CCG'CTC Cuts at: 0 1280 1344 1560 Size: 1280 64 216 Fragments arranged by size: 1280 216 64 BsrFI r'CCGG_y Cuts at: 0 216 234 504 1560 Size: 216 18 270 1056 Fragments arranged by size: 1056 270 216 18 BssHII G'CGCG_C Cuts at: 0 105 838 1560 Size: 105 733 722 Fragments arranged by size: 733 722 105 BstAPI GCAn_nnn'nTGC Cuts at: 0 1412 1560 Size: 1412 148 BstEII G'GTnAC_C Cuts at: 0 669 1560 Size: 669 891 BstYI r'GATC_y Cuts at: 0 881 1560 Size: 881 679 BtgI C'CryG_G Cuts at: 0 167 254 395 1295 1560 Size: 167 87 141 900 265 Fragments arranged by size: 900 265 167 141 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 846 1560 Size: 846 714 ClaI AT'CG_AT Cuts at: 0 938 1560 Size: 938 622 DraIII CAC_nnn'GTG Cuts at: 0 401 1560 Size: 401 1159 EaeI y'GGCC_r Cuts at: 0 218 266 431 557 663 1151 1310 1560 Size: 218 48 165 126 106 488 159 250 Fragments arranged by size: 488 250 218 165 159 126 106 48 EagI C'GGCC_G Cuts at: 0 218 266 1560 Size: 218 48 1294 Fragments arranged by size: 1294 218 48 EarI CTCTTCn'nnn_ Cuts at: 0 1216 1245 1560 Size: 1216 29 315 Fragments arranged by size: 1216 315 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1233 1560 Size: 1233 327 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 397 1330 1560 Size: 97 300 933 230 Fragments arranged by size: 933 300 230 97 EcoO109I rG'GnC_Cy Cuts at: 0 718 1056 1262 1300 1560 Size: 718 338 206 38 260 Fragments arranged by size: 718 338 260 206 38 FspI TGC'GCA Cuts at: 0 871 1560 Size: 871 689 FspAI rTGC'GCAy Cuts at: 0 871 1560 Size: 871 689 HaeII r_GCGC'y Cuts at: 0 77 206 262 635 1329 1560 Size: 77 129 56 373 694 231 Fragments arranged by size: 694 373 231 129 77 56 KasI G'GCGC_C Cuts at: 0 202 258 631 1325 1560 Size: 202 56 373 694 235 Fragments arranged by size: 694 373 235 202 56 KpnI G_GTAC'C Cuts at: 0 1104 1560 Size: 1104 456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 977 1560 Size: 977 583 MscI TGG'CCA Cuts at: 0 559 1560 Size: 559 1001 MslI CAynn'nnrTG Cuts at: 0 679 865 1204 1560 Size: 679 186 339 356 Fragments arranged by size: 679 356 339 186 MspA1I CmG'CkG Cuts at: 0 117 256 589 1170 1297 1560 Size: 117 139 333 581 127 263 Fragments arranged by size: 581 333 263 139 127 117 NaeI GCC'GGC Cuts at: 0 218 236 1560 Size: 218 18 1324 Fragments arranged by size: 1324 218 18 NarI GG'CG_CC Cuts at: 0 203 259 632 1326 1560 Size: 203 56 373 694 234 Fragments arranged by size: 694 373 234 203 56 NgoMIV G'CCGG_C Cuts at: 0 216 234 1560 Size: 216 18 1326 Fragments arranged by size: 1326 216 18 PmlI CAC'GTG Cuts at: 0 429 1560 Size: 429 1131 PpuMI rG'GwC_Cy Cuts at: 0 1300 1560 Size: 1300 260 PspOMI G'GGCC_C Cuts at: 0 717 1261 1560 Size: 717 544 299 Fragments arranged by size: 717 544 299 PspXI vC'TCGA_Gb Cuts at: 0 886 1560 Size: 886 674 PvuII CAG'CTG Cuts at: 0 117 1560 Size: 117 1443 SacII CC_GC'GG Cuts at: 0 257 1298 1560 Size: 257 1041 262 Fragments arranged by size: 1041 262 257 SanDI GG'GwC_CC Cuts at: 0 1300 1560 Size: 1300 260 SapI GCTCTTCn'nnn_ Cuts at: 0 1216 1560 Size: 1216 344 SfiI GGCCn_nnn'nGGCC Cuts at: 0 475 1560 Size: 475 1085 SfoI GGC'GCC Cuts at: 0 204 260 633 1327 1560 Size: 204 56 373 694 233 Fragments arranged by size: 694 373 233 204 56 SgrAI Cr'CCGG_yG Cuts at: 0 504 1560 Size: 504 1056 SmaI CCC'GGG Cuts at: 0 1268 1334 1560 Size: 1268 66 226 Fragments arranged by size: 1268 226 66 SmlI C'TyrA_G Cuts at: 0 471 886 1560 Size: 471 415 674 Fragments arranged by size: 674 471 415 SspI AAT'ATT Cuts at: 0 1553 1560 Size: 1553 7 StuI AGG'CCT Cuts at: 0 469 1560 Size: 469 1091 StyI C'CwwG_G Cuts at: 0 1247 1560 Size: 1247 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 98 1057 1560 Size: 98 959 503 Fragments arranged by size: 959 503 98 TatI w'GTAC_w Cuts at: 0 890 1139 1560 Size: 890 249 421 Fragments arranged by size: 890 421 249 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1495 1527 1560 Size: 1495 32 33 Fragments arranged by size: 1495 33 32 XhoI C'TCGA_G Cuts at: 0 886 1560 Size: 886 674 XmaI C'CCGG_G Cuts at: 0 1266 1332 1560 Size: 1266 66 228 Fragments arranged by size: 1266 228 66 Enzymes that do cut: Acc65I AcuI AfeI AgeI AleI AloI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssHII BstAPI BstEII BstYI BtgI BtgZI ClaI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PmlI PpuMI PspOMI PspXI PvuII SacII SanDI SapI SfiI SfoI SgrAI SmaI SmlI SspI StuI StyI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BsaI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I BtsI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI XmnI ZraI