Restriction map for
pFN21AB9722
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI StuI StyI SwaI
TfiI Tru9I Tsp509I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 75 GTAC GT^AC
Alw44I 450 GTGCAC G^TGCAC
AlwI 864 GATCC GGATC(4/5)
AlwNI 559 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 621 GGGCCC GGGCC^C
ApaLI 450 GTGCAC G^TGCAC
AspI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 477 GGTGCC G^G[CT][GA]CC
BbrPI 61 CACGTG CAC^GTG
BbsI 275 GTCTTC GAAGAC(2/6)
BglI 623 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 275 GTCTTC GAAGAC(2/6)
BsaAI 61 CACGTG [CT]AC^GT[GA]
BsaWI 414 ACCGGA [AT]^CCGG[AT]
BsiEI 458 CGGCCG CG[GA][CT]^CG
BsmAI 361 GTCTC GTCTC(1/5)
BsmBI 360 CGTCTC CGTCTC(1/5)
BsmI 781 GCATTC GAATGC(1/-1)
BspMI 902 GCAGGT ACCTGC(4/8)
BstEII 822 GGTGACC G^GT[ACGT]ACC
BstPI 822 GGTGACC G^GT[ACGT]ACC
BstYI 863 AGATCC [GA]^GATC[CT]
EagI 458 CGGCCG C^GGCCG
EclXI 458 CGGCCG C^GGCCG
Eco52I 458 CGGCCG C^GGCCG
EcoO65I 822 GGTGACC G^GT[ACGT]ACC
KspI 766 CCGCGG CCGC^GG
MaeIII 823 GTGAC ^GT[ACGT]AC
MflI 863 AGATCC [GA]^GATC[CT]
NspI 38 GCATGC [GA]CATG^[CT]
PflFI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 558 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 61 CACGTG CAC^GTG
PmlI 61 CACGTG CAC^GTG
PpuMI 770 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 75 GTAC GT^AC
SacII 766 CCGCGG CCGC^GG
SbfI 957 CCTGCAGG CCTGCA^GG
ScaI 74 AGTACT AGT^ACT
SfaNI 809 GCATC GCATC(5/9)
SmaI 595 CCCGGG CCC^GGG
SphI 38 GCATGC GCATG^C
Sse8387I 957 CCTGCAGG CCTGCA^GG
SspI 1214 AATATT AAT^ATT
Tsp45I 823 GTGAC ^GT[GC]AC
Tth111I 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 558 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 863 AGATCC [GA]^GATC[CT]
XmaCI 595 CCCGGG C^CCGGG
XmaI 595 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 418 GACGCC G[GA]^CG[CT]C
757 GACGCC
AvaII 771 GGACC G^G[AT]CC
1149 GGACC
BalI 668 TGGCCA TGG^CCA
851 TGGCCA
BcgI 293 GCAATATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1049 GCACGTCCATCG
BlpI 699 GCTCAGC GC^T[ACGT]AGC
882 GCTTAGC
Bpu1102I 699 GCTCAGC GC^T[ACGT]AGC
882 GCTTAGC
BsaHI 418 GACGCC G[GA]^CG[CT]C
757 GACGCC
BsgI 58 CTGCAC GTGCAG(16/14)
85 CTGCAC
BsrFI 648 GCCGGC [GA]^CCGG[CT]
1222 GCCGGC
BstAPI 717 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
893 GCACCATCTGC
CelII 699 GCTCAGC GC^T[ACGT]AGC
882 GCTTAGC
Cfr10I 648 GCCGGC [GA]^CCGG[CT]
1222 GCCGGC
DraII 621 GGGCCCC [GA]G^G[ACGT]CC[CT]
770 GGGACCT
DsaI 350 CCGTGG C^C[GA][CT]GG
766 CCGCGG
EarI 465 CTCTTC CTCTTC(1/4)
1018 CTCTTC
EcoO109I 621 GGGCCCC [GA]G^G[ACGT]CC[CT]
770 GGGACCT
Hin1I 418 GACGCC G[GA]^CG[CT]C
757 GACGCC
Ksp632I 465 CTCTTC CTCTTC(1/4)
1018 CTCTTC
MluNI 668 TGGCCA TGG^CCA
851 TGGCCA
MscI 668 TGGCCA TGG^CCA
851 TGGCCA
MspA1I 237 CAGCGG C[AC]G^C[GT]G
766 CCGCGG
NaeI 648 GCCGGC GCC^GGC
1222 GCCGGC
NgoMIV 648 GCCGGC G^CCGGC
1222 GCCGGC
PaeR7I 147 CTCGAG C^TCGAG
970 CTCGAG
PstI 900 CTGCAG CTGCA^G
958 CTGCAG
SfcI 900 CTGCAG C^T[GA][CT]AG
958 CTGCAG
XhoI 147 CTCGAG C^TCGAG
970 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI Aor51HI AspHI
AvaI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI Eco47III
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SmlI TaqI TseI TspRI TthHB8I