Restriction map for
pFN21AB9722
(Circular) (Six-base) MAPSORT of: pFN21AB9722.seq Check: 4558 from: 1 to: 5935
pFN21AB9722 5935 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3179 3998 5382 5650 1632
Size: 1547 819 1384 268 1917
Fragments arranged by size:
1917 1547 1384 819 268
AfeI AGC'GCT
Cuts at: 2703 2798 3080 2703
Size: 95 282 5558
Fragments arranged by size:
5558 282 95
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2569 3678 5250 2569
Size: 1109 1572 3254
Fragments arranged by size:
3254 1572 1109
ApaI G_GGCC'C
Cuts at: 2630 2630
Size: 5935
ApaLI G'TGCA_C
Cuts at: 2455 3992 5148 2455
Size: 1537 1156 3242
Fragments arranged by size:
3242 1537 1156
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2600 2975 3260 1870
Size: 73 209 448 375 285 4545
Fragments arranged by size:
4545 448 375 285 209 73
BamHI G'GATC_C
Cuts at: 3265 3632 3265
Size: 367 5568
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2482 3256 4711 618
Size: 325 217 627 695 774 1455 1842
Fragments arranged by size:
1842 1455 774 695 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2076 2111 2258 2630 2849 2885 3254 1391
Size: 685 35 147 372 219 36 369 4072
Fragments arranged by size:
4072 685 372 369 219 147 36 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2273 928
Size: 971 374 4590
Fragments arranged by size:
4590 971 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2287 2321 3043 3077 4126 4160 1903
Size: 34 350 34 722 34 1049 34 3678
Fragments arranged by size:
3678 1049 722 350 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2634 4546 136
Size: 107 122 71 631 605 962 1912 1525
Fragments arranged by size:
1912 1525 962 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2705 2888 3352 2705
Size: 183 464 5288
Fragments arranged by size:
5288 464 183
Bme1580I G_kGCm'C
Cuts at: 1790 2459 2487 2516 2630 3996 5152 1790
Size: 669 28 29 114 1366 1156 2573
Fragments arranged by size:
2573 1366 1156 669 114 29 28
BmgBI CAC'GTC
Cuts at: 1318 2460 2758 3057 1318
Size: 1142 298 299 4196
Fragments arranged by size:
4196 1142 299 298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2209 2591 2606 2801 4595 1239
Size: 970 382 15 195 1794 2579
Fragments arranged by size:
2579 1794 970 382 195 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 4060 4925 5223 2849
Size: 1211 865 298 3561
Fragments arranged by size:
3561 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4598 882
Size: 617 3099 2219
Fragments arranged by size:
3099 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 3726 493
Size: 711 864 1658 2702
Fragments arranged by size:
2702 1658 864 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2424 2763 3742 4124
Size: 53 83 186 936 891 339 979 382
Cuts at: 4124 4759 275
Size: 635 1451
Fragments arranged by size:
1451 979 936 891 635 382 339 186
83 53
BsaWI w'CCGG_w
Cuts at: 2419 4368 4766 5040 5187 5598 2419
Size: 1949 398 274 147 411 2756
Fragments arranged by size:
2756 1949 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 2263 2293 2351
Size: 30 165 30 660 30 170 30 58
Cuts at: 2351 2381 1178
Size: 30 4732
Fragments arranged by size:
4732 660 170 165 58 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2993 3109 1186
Size: 915 892 116 4012
Fragments arranged by size:
4012 915 892 116
BseYI C'CCAG_C
Cuts at: 2144 2821 5138 5634 2144
Size: 677 2317 496 2445
Fragments arranged by size:
2445 2317 677 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2075 1163
Size: 573 312 27 5023
Fragments arranged by size:
5023 573 312 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2466 3385 4146 5174 1540
Size: 275 186 465 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 465 275 186
BsiHKAI G_wGCw'C
Cuts at: 2459 2592 2900 3996 4081 5152 2459
Size: 133 308 1096 85 1071 3242
Fragments arranged by size:
3242 1096 1071 308 133 85
BsmI GAATG_Cn'
Cuts at: 2786 3446 3539 2786
Size: 660 93 5182
Fragments arranged by size:
5182 660 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2371 2371
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2111 2258 2459 2487 2516 2592
Size: 399 286 35 147 201 28 29 76
Cuts at: 2592 2630 2849 2885 2900 3254 3996 4081 5152
Size: 38 219 36 15 354 742 85 1071
Cuts at: 5152 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 354 286 219 201
147 85 76 38 36 35 29 28
15
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3290 844
Size: 2054 392 3489
Fragments arranged by size:
3489 2054 392
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2653 3227 4579 4766 1700
Size: 110 843 574 1352 187 2869
Fragments arranged by size:
2869 1352 843 574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2728 2904 794
Size: 1934 176 3825
Fragments arranged by size:
3825 1934 176
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 4292
BstYI r'GATC_y
Cuts at: 1972 2868 3265 3632 4017 4034 5486 5572 5584
Size: 896 397 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 896 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2355 2771 513
Size: 552 1290 416 3677
Fragments arranged by size:
3677 1290 552 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2107 3046 3124 487
Size: 683 56 881 939 78 3298
Fragments arranged by size:
3298 939 881 683 78 56
BtsI GCAGTG_nn'
Cuts at: 2087 2239 3451 4245 4265 2087
Size: 152 1212 794 20 3757
Fragments arranged by size:
3757 1212 794 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2146 2181 529
Size: 35 1582 35 4283
Fragments arranged by size:
4283 1582 35 35
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2463 2673
Size: 54 1425 50 275 18 303 330 210
Cuts at: 2673 2856 3382 4271 5900 8
Size: 183 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 330 303 275 210
183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2463 3382 1537
Size: 275 651 919 4090
Fragments arranged by size:
4090 919 651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2476 3029 3865 1353
Size: 1123 553 836 3423
Fragments arranged by size:
3423 1123 836 553
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2220 3214 4522 4896 5042 387
Size: 1064 138 631 994 1308 374 146 1280
Fragments arranged by size:
1308 1280 1064 994 631 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2591 2606 2801 3179 3998 4595
Size: 393 577 382 15 195 378 819 597
Cuts at: 4595 5382 5650 1239
Size: 787 268 1524
Fragments arranged by size:
1524 819 787 597 577 393 382 378
268 195 15
EcoO109I rG'GnC_Cy
Cuts at: 2627 2776 2627
Size: 149 5786
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 2570 2619 2640 2705 2800 3082 5082 1678
Size: 892 49 21 65 95 282 2000 2531
Fragments arranged by size:
2531 2000 892 282 95 65 49 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2263 2295 2351 2383 2831
Size: 32 135 32 691 32 56 32 448
Cuts at: 2831 2863 4576 4608 4650 4682 1373
Size: 32 1713 32 42 32 2626
Fragments arranged by size:
2626 1713 691 448 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2309 5049 5233 1305
Size: 1004 2740 184 2007
Fragments arranged by size:
2740 2007 1004 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 2858 10
Size: 54 1425 343 843 183 3087
Fragments arranged by size:
3087 1425 843 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3893 4252 4411 518
Size: 648 843 1884 359 159 2042
Fragments arranged by size:
2042 1884 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2244 2773 3351 4028 5176 5421 1606
Size: 638 529 578 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 578 529 245
NaeI GCC'GGC
Cuts at: 2655 3229 2655
Size: 574 5361
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5383
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NgoMIV G'CCGG_C
Cuts at: 2653 3227 2653
Size: 574 5361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2047 3293 4838 1132
Size: 97 818 1246 1545 2229
Fragments arranged by size:
2229 1545 1246 818 97
PasI CC'CwG_GG
Cuts at: 1348 2611 1348
Size: 1263 4672
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2569 1108
Size: 1461 4474
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PmlI CAC'GTG
Cuts at: 2068 2068
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 2776
Size: 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspOMI G'GGCC_C
Cuts at: 2626 2626
Size: 5935
PspXI vC'TCGA_Gb
Cuts at: 2152 2975 2152
Size: 823 5112
PstI C_TGCA'G
Cuts at: 830 2909 2967 3287 830
Size: 2079 58 320 3478
Fragments arranged by size:
3478 2079 320 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
SacII CC_GC'GG
Cuts at: 2774 2774
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 2967 3287 2967
Size: 320 5615
ScaI AGT'ACT
Cuts at: 2081 4183 5895 2081
Size: 2102 1712 2121
Fragments arranged by size:
2121 2102 1712
SfcI C'TryA_G
Cuts at: 826 1045 2905 2963 3283 4418 5099 5290 826
Size: 219 1860 58 320 1135 681 191 1471
Fragments arranged by size:
1860 1471 1135 681 320 219 191 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2602 3262 1872
Size: 730 660 4545
Fragments arranged by size:
4545 730 660
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2152 2864 2975 4039 4940 5202
Size: 197 926 209 712 111 1064 901 262
Cuts at: 5202 820
Size: 1553
Fragments arranged by size:
1553 1064 926 901 712 262 209 197
111
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 2047 3293 1132
Size: 915 1246 3774
Fragments arranged by size:
3774 1246 915
SspI AAT'ATT
Cuts at: 5 52 3221 3859 5
Size: 47 3169 638 2081
Fragments arranged by size:
3169 2081 638 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 3374 513
Size: 552 2309 3074
Fragments arranged by size:
3074 2309 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 4181 5893
Size: 274 80 33 51 1443 102 2102 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
2102 1712 1443 274 138 102 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 1316
Size: 1047 4888
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 2152 2975 1943
Size: 209 823 4903
Fragments arranged by size:
4903 823 209
XmaI C'CCGG_G
Cuts at: 1870 2600 3260 1870
Size: 730 660 4545
Fragments arranged by size:
4545 730 660
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI