Restriction map for
pFN21AB9722
(Linear) (Six-base) MAPSORT of: KIBB9722.seq Check: 8142 from: 1 to: 1230
KIBB9722.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1230
Size: 1174 56
AfeI AGC'GCT
Cuts at: 0 698 793 1075 1230
Size: 698 95 282 155
Fragments arranged by size:
698 282 155 95
AlwNI CAG_nnn'CTG
Cuts at: 0 564 1230
Size: 564 666
ApaI G_GGCC'C
Cuts at: 0 625 1230
Size: 625 605
ApaLI G'TGCA_C
Cuts at: 0 450 1230
Size: 450 780
AvaI C'yCGr_G
Cuts at: 0 147 595 970 1230
Size: 147 448 375 260
Fragments arranged by size:
448 375 260 147
BanI G'GyrC_C
Cuts at: 0 477 1230
Size: 477 753
BanII G_rGCy'C
Cuts at: 0 71 106 253 625 844 880 1230
Size: 71 35 147 372 219 36 350
Fragments arranged by size:
372 350 219 147 71 36 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 268 1230
Size: 268 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 282 316 1038 1072 1230
Size: 282 34 722 34 158
Fragments arranged by size:
722 282 158 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 629 1230
Size: 629 601
BlpI GC'TnA_GC
Cuts at: 0 700 883 1230
Size: 700 183 347
Fragments arranged by size:
700 347 183
Bme1580I G_kGCm'C
Cuts at: 0 454 482 511 625 1230
Size: 454 28 29 114 605
Fragments arranged by size:
605 454 114 29 28
BmgBI CAC'GTC
Cuts at: 0 455 753 1052 1230
Size: 455 298 299 178
Fragments arranged by size:
455 299 298 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1092 1230
Size: 1092 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 586 601 796 1230
Size: 204 382 15 195 434
Fragments arranged by size:
434 382 204 195 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1230
Size: 844 386
BsaAI yAC'GTr
Cuts at: 0 63 1230
Size: 63 1167
BsaHI Gr'CG_yC
Cuts at: 0 419 758 1230
Size: 419 339 472
Fragments arranged by size:
472 419 339
BsaWI w'CCGG_w
Cuts at: 0 414 1230
Size: 414 816
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 258 288 346 376 1230
Size: 58 30 170 30 58 30 854
Fragments arranged by size:
854 170 58 58 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 988 1104 1230
Size: 96 892 116 126
Fragments arranged by size:
892 126 116 96
BseYI C'CCAG_C
Cuts at: 0 139 816 1230
Size: 139 677 414
Fragments arranged by size:
677 414 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 70 1230
Size: 43 27 1160
Fragments arranged by size:
1160 43 27
BsiEI CG_ry'CG
Cuts at: 0 461 1230
Size: 461 769
BsiHKAI G_wGCw'C
Cuts at: 0 454 587 895 1230
Size: 454 133 308 335
Fragments arranged by size:
454 335 308 133
BsmI GAATG_Cn'
Cuts at: 0 781 1230
Size: 781 449
BsmBI CGTCTCn'nnnn_
Cuts at: 0 366 1230
Size: 366 864
Bsp1286I G_dGCh'C
Cuts at: 0 71 106 253 454 482 511 587 625
Size: 71 35 147 201 28 29 76 38
Cuts at: 625 844 880 895 1230
Size: 219 36 15 335
Fragments arranged by size:
335 219 201 147 76 71 38 36
35 29 28 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1230
Size: 893 337
BsrFI r'CCGG_y
Cuts at: 0 648 1222 1230
Size: 648 574 8
Fragments arranged by size:
648 574 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 723 899 1230
Size: 723 176 331
Fragments arranged by size:
723 331 176
BstEII G'GTnAC_C
Cuts at: 0 822 1230
Size: 822 408
BstYI r'GATC_y
Cuts at: 0 863 1230
Size: 863 367
BtgI C'CryG_G
Cuts at: 0 350 766 1230
Size: 350 416 464
Fragments arranged by size:
464 416 350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 102 1041 1119 1230
Size: 102 939 78 111
Fragments arranged by size:
939 111 102 78
BtsI GCAGTG_nn'
Cuts at: 0 82 234 1230
Size: 82 152 996
Fragments arranged by size:
996 152 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 141 176 1230
Size: 141 35 1054
Fragments arranged by size:
1054 141 35
EaeI y'GGCC_r
Cuts at: 0 128 458 668 851 1230
Size: 128 330 210 183 379
Fragments arranged by size:
379 330 210 183 128
EagI C'GGCC_G
Cuts at: 0 458 1230
Size: 458 772
EarI CTCTTCn'nnn_
Cuts at: 0 471 1024 1230
Size: 471 553 206
Fragments arranged by size:
553 471 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 215 1209 1230
Size: 215 994 21
Fragments arranged by size:
994 215 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 586 601 796 1174 1230
Size: 204 382 15 195 378 56
Fragments arranged by size:
382 378 204 195 56 15
EcoO109I rG'GnC_Cy
Cuts at: 0 622 771 1230
Size: 622 149 459
Fragments arranged by size:
622 459 149
HaeII r_GCGC'y
Cuts at: 0 565 614 635 700 795 1077 1230
Size: 565 49 21 65 95 282 153
Fragments arranged by size:
565 282 153 95 65 49 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 258 290 346 378 826 858 1230
Size: 258 32 56 32 448 32 372
Fragments arranged by size:
448 372 258 56 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1230
Size: 304 926
MscI TGG'CCA
Cuts at: 0 670 853 1230
Size: 670 183 377
Fragments arranged by size:
670 377 183
MspA1I CmG'CkG
Cuts at: 0 239 768 1230
Size: 239 529 462
Fragments arranged by size:
529 462 239
NaeI GCC'GGC
Cuts at: 0 650 1224 1230
Size: 650 574 6
Fragments arranged by size:
650 574 6
NgoMIV G'CCGG_C
Cuts at: 0 648 1222 1230
Size: 648 574 8
Fragments arranged by size:
648 574 8
NspI r_CATG'y
Cuts at: 0 42 1230
Size: 42 1188
PasI CC'CwG_GG
Cuts at: 0 606 1230
Size: 606 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 564 1230
Size: 564 666
PmlI CAC'GTG
Cuts at: 0 63 1230
Size: 63 1167
PpuMI rG'GwC_Cy
Cuts at: 0 771 1230
Size: 771 459
PspOMI G'GGCC_C
Cuts at: 0 621 1230
Size: 621 609
PspXI vC'TCGA_Gb
Cuts at: 0 147 970 1230
Size: 147 823 260
Fragments arranged by size:
823 260 147
PstI C_TGCA'G
Cuts at: 0 904 962 1230
Size: 904 58 268
Fragments arranged by size:
904 268 58
SacII CC_GC'GG
Cuts at: 0 769 1230
Size: 769 461
SbfI CC_TGCA'GG
Cuts at: 0 962 1230
Size: 962 268
ScaI AGT'ACT
Cuts at: 0 76 1230
Size: 76 1154
SfcI C'TryA_G
Cuts at: 0 900 958 1230
Size: 900 58 272
Fragments arranged by size:
900 272 58
SmaI CCC'GGG
Cuts at: 0 597 1230
Size: 597 633
SmlI C'TyrA_G
Cuts at: 0 147 859 970 1230
Size: 147 712 111 260
Fragments arranged by size:
712 260 147 111
SphI G_CATG'C
Cuts at: 0 42 1230
Size: 42 1188
SspI AAT'ATT
Cuts at: 0 1216 1230
Size: 1216 14
TatI w'GTAC_w
Cuts at: 0 74 1230
Size: 74 1156
Tth111I GACn'n_nGTC
Cuts at: 0 358 1230
Size: 358 872
XhoI C'TCGA_G
Cuts at: 0 147 970 1230
Size: 147 823 260
Fragments arranged by size:
823 260 147
XmaI C'CCGG_G
Cuts at: 0 595 1230
Size: 595 635
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI
BstEII BstYI BtgI BtgZI BtsI CspCI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I MmeI MscI
MspA1I NaeI NgoMIV NspI PasI PflMI PmlI PpuMI
PspOMI PspXI PstI SacII SbfI ScaI SfcI SmaI
SmlI SphI SspI TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BmrI BmtI Bpu10I BsaI BsaBI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StuI StyI SwaI TaqII
TstI XbaI XcmI XmnI ZraI