################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspHI AspI AvaII AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraII DrdI DsaI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MamI MfeI MflI MluNI MroI MscI MseI MunI NcoI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AhdI 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 587 GGATC GGATC(4/5) AocI 262 CCTCAGG CC^T[ACGT]AGG AscI 303 GGCGCGCC GG^CGCGCC AspEI 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 5 CGACTTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 292 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 808 GTGCAG GTGCAG(16/14) BsmAI 723 GTCTC GTCTC(1/5) BsmFI 668 GGGAC GGGAC(10/14) BssHII 304 GCGCGC G^CGCGC BstEII 909 GGTAACC G^GT[ACGT]ACC BstPI 909 GGTAACC G^GT[ACGT]ACC Bsu36I 262 CCTCAGG CC^T[ACGT]AGG DpnII 588 GATC ^GATC Eam1105I 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 262 CCTCAGG CC^T[ACGT]AGG EcoO65I 909 GGTAACC G^GT[ACGT]ACC MboI 588 GATC ^GATC MboII 945 GAAGA GAAGA(8/7) MluI 789 ACGCGT A^CGCGT MslI 801 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 588 GATC ^GATC NheI 841 GCTAGC G^CTAGC NlaIII 168 CATG CATG^ PflMI 659 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 588 GATC ^GATC SgrAI 868 CGCCGGCG C[GA]^CCGG[CT]G Van91I 659 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 344 CCAGCGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 48 GTAC GT^AC 681 GTAC AflIII 569 ACGTGT A^C[GA][CT]GT 789 ACGCGT AlwNI 183 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 198 CAGCCACTG BanII 147 GAGCCC G[GA]GC[CT]^C 650 GGGCTC BseRI 98 GAGGAG GAGGAG(10/8) 101 GAGGAG BspMI 212 ACCTGC ACCTGC(4/8) 416 ACCTGC BsrBI 75 GAGCGG CCGCTC(-3/-3) 122 GAGCGG BsrI 647 ACTGG ACTGG(1/-1) 309 CCAGT BstAPI 182 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 222 GCACCCCGTGC DraIII 223 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 406 CACAACGTG EaeI 31 TGGCCG [CT]^GGCC[GA] 194 CGGCCA EcoT14I 506 CCAAGG C^C[AT][AT]GG 689 CCAAGG HgaI 616 GACGC GACGC(5/10) 609 GCGTC NaeI 243 GCCGGC GCC^GGC 869 GCCGGC NgoMIV 243 GCCGGC G^CCGGC 869 GCCGGC PvuII 186 CAGCTG CAG^CTG 811 CAGCTG RsaI 48 GTAC GT^AC 681 GTAC SmaI 444 CCCGGG CCC^GGG 583 CCCGGG StyI 506 CCAAGG C^C[AT][AT]GG 689 CCAAGG TaqI 632 TCGA T^CGA 964 TCGA Tsp45I 485 GTGAC ^GT[GC]AC 611 GTCAC TthHB8I 632 TCGA T^CGA 964 TCGA XmaCI 444 CCCGGG C^CCGGG 583 CCCGGG XmaI 444 CCCGGG C^CCGGG 583 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AluI AvaI BanI BbeI BbvI BcnI BfaI BmyI BsaHI BsaJI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeI MaeII MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV PstI Sau96I ScrFI SfaNI SfcI SfoI TseI TspRI