(Circular) (Six-base) MAPSORT of: pFN21AB8954.seq Check: 2952 from: 1 to: 5695 pFN21AB8954 5695 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2840 3758 5142 5410 1632 Size: 1208 918 1384 268 1917 Fragments arranged by size: 1917 1384 1208 918 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2574 2794 4594 5571 1203 Size: 1371 220 1800 977 1327 Fragments arranged by size: 1800 1371 1327 977 220 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 2497 4424 2497 Size: 1927 3768 AleI CACnn'nnGTG Cuts at: 1166 2810 1166 Size: 1644 4051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2193 2208 3438 5010 2193 Size: 15 1230 1572 2878 Fragments arranged by size: 2878 1572 1230 15 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AscI GG'CGCG_CC Cuts at: 2309 2309 Size: 5695 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2449 2588 2759 3020 1870 Size: 73 224 282 139 171 261 4545 Fragments arranged by size: 4545 282 261 224 171 139 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2273 2477 2584 2601 Size: 325 217 627 474 12 204 107 17 Cuts at: 2601 3016 4471 618 Size: 415 1455 1842 Fragments arranged by size: 1842 1455 627 474 415 325 217 204 107 17 12 BanII G_rGCy'C Cuts at: 1391 2156 2659 3014 1391 Size: 765 503 355 4072 Fragments arranged by size: 4072 765 503 355 BbeI G_GCGC'C Cuts at: 2265 2277 2481 2265 Size: 12 204 5479 Fragments arranged by size: 5479 204 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1999 2033 3886 3920 1903 Size: 34 62 34 1853 34 3678 Fragments arranged by size: 3678 1853 62 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 4306 136 Size: 107 122 71 631 605 631 2003 1525 Fragments arranged by size: 2003 1525 631 631 605 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2323 2589 2604 2802 3756 4912 1790 Size: 533 266 15 198 954 1156 2573 Fragments arranged by size: 2573 1156 954 533 266 198 15 BmgBI CAC'GTC Cuts at: 1318 2575 2743 1318 Size: 1257 168 4270 Fragments arranged by size: 4270 1257 168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2662 3426 4384 Size: 416 53 83 399 24 1245 764 958 Cuts at: 4384 442 Size: 1753 Fragments arranged by size: 1753 1245 958 764 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2850 1056 Size: 1794 3901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4355 1239 Size: 3116 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 2274 2478 2622 Size: 53 83 186 936 729 12 204 144 Cuts at: 2622 3502 3884 4519 275 Size: 880 382 635 1451 Fragments arranged by size: 1451 936 880 729 635 382 204 186 144 83 53 12 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2118 2121 1186 Size: 932 3 4760 Fragments arranged by size: 4760 932 3 BseYI C'CCAG_C Cuts at: 2236 2531 2670 4898 5394 2236 Size: 295 139 2228 496 2537 Fragments arranged by size: 2537 2228 496 295 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2834 1163 Size: 573 1098 4024 Fragments arranged by size: 4024 1098 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3756 3841 4912 3756 Size: 85 1071 4539 Fragments arranged by size: 4539 1071 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2323 2589 2604 2659 2802 3014 Size: 399 366 167 266 15 55 143 212 Cuts at: 3014 3756 3841 4912 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 266 212 167 143 85 55 15 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 2430 3050 844 Size: 1382 204 620 3489 Fragments arranged by size: 3489 1382 620 204 BsrBI CCG'CTC Cuts at: 1394 1823 2082 2129 3582 1394 Size: 429 259 47 1453 3507 Fragments arranged by size: 3507 1453 429 259 47 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2248 2775 2874 2934 4339 4526 1700 Size: 110 438 527 99 60 1405 187 2869 Fragments arranged by size: 2869 1405 527 438 187 110 99 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 2309 1925 Size: 384 5311 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 2233 794 Size: 1399 40 4256 Fragments arranged by size: 4256 1399 40 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2914 1184 Size: 1730 3965 BstYI r'GATC_y Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690 Size: 1053 367 385 17 1452 86 12 346 Cuts at: 5690 1972 Size: 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2268 2268 Size: 5695 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2570 487 Size: 683 56 1344 3612 Fragments arranged by size: 3612 1344 683 56 BtsI GCAGTG_nn' Cuts at: 2206 3211 4005 4025 2206 Size: 1005 794 20 3876 Fragments arranged by size: 3876 1005 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2233 2416 2233 Size: 183 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2199 3142 Size: 54 1425 50 275 18 206 163 943 Cuts at: 3142 4031 5660 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 943 889 275 206 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 2115 2139 2701 2875 4282 Size: 1064 138 501 25 24 562 174 1407 Cuts at: 4282 4656 4802 387 Size: 374 146 1280 Fragments arranged by size: 1407 1280 1064 562 501 374 174 146 138 25 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2840 3758 4355 5142 5410 1239 Size: 393 1208 918 597 787 268 1524 Fragments arranged by size: 1524 1208 918 787 597 393 268 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2265 2277 2355 2481 2875 2911 2978 4842 Size: 587 12 78 126 394 36 67 1864 Cuts at: 4842 1678 Size: 2531 Fragments arranged by size: 2531 1864 587 394 126 78 67 36 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2486 2515 2518 4336 Size: 32 135 32 911 3 29 3 1818 Cuts at: 4336 4368 4410 4442 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1818 911 135 42 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2261 2273 2477 2261 Size: 12 204 5479 Fragments arranged by size: 5479 204 12 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 2794 5571 2794 Size: 2777 2918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3653 4012 4171 518 Size: 648 1644 843 359 159 2042 Fragments arranged by size: 2042 1644 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2145 2193 2598 2818 3111 3788 4936 5181 Size: 539 48 405 220 293 677 1148 245 Cuts at: 5181 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 539 405 293 245 220 48 NaeI GCC'GGC Cuts at: 2250 2876 2250 Size: 626 5069 NarI GG'CG_CC Cuts at: 2262 2274 2478 2262 Size: 12 204 5479 Fragments arranged by size: 5479 204 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5143 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2248 2874 2248 Size: 626 5069 NheI G'CTAG_C Cuts at: 1052 2846 1052 Size: 1794 3901 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 2257 2359 1348 Size: 909 102 4684 Fragments arranged by size: 4684 909 102 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2670 1108 Size: 1562 4133 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PstI C_TGCA'G Cuts at: 830 2144 2427 2757 3047 830 Size: 1314 283 330 290 3478 Fragments arranged by size: 3478 1314 330 290 283 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2193 2818 2193 Size: 625 5070 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2140 2423 2753 3043 4178 4859 5050 Size: 219 1095 283 330 290 1135 681 191 Cuts at: 5050 826 Size: 1471 Fragments arranged by size: 1471 1135 1095 681 330 290 283 219 191 SfoI GGC'GCC Cuts at: 2263 2275 2479 2263 Size: 12 204 5479 Fragments arranged by size: 5479 204 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 2874 1700 Size: 1174 4521 SmaI CCC'GGG Cuts at: 1872 2451 2590 3022 1872 Size: 579 139 432 4545 Fragments arranged by size: 4545 579 432 139 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4700 4962 820 Size: 197 926 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5695 StyI C'CwwG_G Cuts at: 513 1065 2511 2694 3134 513 Size: 552 1446 183 440 3074 Fragments arranged by size: 3074 1446 552 440 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 3941 5653 Size: 274 80 33 51 1443 708 1256 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1443 1256 708 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2449 2588 3020 1870 Size: 579 139 432 4545 Fragments arranged by size: 4545 579 432 139 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI