(Circular) (Six-base) MAPSORT of: pFN21AB8943.seq Check: 1570 from: 1 to: 5608 pFN21AB8943 5608 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2438 2438 Size: 5608 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2356 3671 5055 5323 1632 Size: 724 1315 1384 268 1917 Fragments arranged by size: 1917 1384 1315 724 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 2387 1166 Size: 1221 4387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2405 2427 1184 Size: 1221 22 4365 Fragments arranged by size: 4365 1221 22 AlwNI CAG_nnn'CTG Cuts at: 2224 2379 2430 3351 4923 2224 Size: 155 51 921 1572 2909 Fragments arranged by size: 2909 1572 921 155 51 ApaI G_GGCC'C Cuts at: 2131 2131 Size: 5608 ApaLI G'TGCA_C Cuts at: 2402 2465 3665 4821 2402 Size: 63 1200 1156 3189 Fragments arranged by size: 3189 1200 1156 63 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2045 2122 2695 2933 1870 Size: 73 86 16 77 573 238 4545 Fragments arranged by size: 4545 573 238 86 77 73 16 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 2929 4384 618 Size: 325 217 627 947 195 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 195 BanII G_rGCy'C Cuts at: 1391 2019 2121 2131 2823 2927 1391 Size: 628 102 10 692 104 4072 Fragments arranged by size: 4072 692 628 104 102 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2752 928 Size: 971 853 3784 Fragments arranged by size: 3784 971 853 BbvCI CC'TCA_GC Cuts at: 2571 2571 Size: 5608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3799 3833 1903 Size: 34 50 34 1778 34 3678 Fragments arranged by size: 3678 1778 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 2421 1548 Size: 873 4735 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 4219 136 Size: 107 122 71 631 605 637 1910 1525 Fragments arranged by size: 1910 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2131 2406 2469 2739 3669 4825 1790 Size: 341 275 63 270 930 1156 2573 Fragments arranged by size: 2573 1156 930 341 275 270 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 4297 442 Size: 416 53 83 399 24 1922 958 1753 Fragments arranged by size: 1922 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2254 4268 1239 Size: 1015 2014 2579 Fragments arranged by size: 2579 2014 1015 Bpu10I CC'TnA_GC Cuts at: 2571 2571 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2266 2594 3733 4598 4896 2266 Size: 328 1139 865 298 2978 Fragments arranged by size: 2978 1139 865 328 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 2753 5602 2753 Size: 2849 2759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2888 3415 3797 4432 Size: 53 83 186 936 1355 527 382 635 Cuts at: 4432 275 Size: 1451 Fragments arranged by size: 1451 1355 936 635 527 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 4041 4439 4713 4860 5271 2011 Size: 2030 398 274 147 411 2348 Fragments arranged by size: 2348 2030 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 1178 Size: 30 165 30 792 30 4561 Fragments arranged by size: 4561 792 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5608 BseYI C'CCAG_C Cuts at: 2589 4811 5307 2589 Size: 2222 496 2890 Fragments arranged by size: 2890 2222 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2365 1163 Size: 573 609 20 4406 Fragments arranged by size: 4406 609 573 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3058 3819 4847 1540 Size: 275 186 805 252 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2211 2406 2469 2823 3669 3754 4825 2211 Size: 195 63 354 846 85 1071 2994 Fragments arranged by size: 2994 1071 846 354 195 85 63 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2121 2131 2211 2406 2469 2739 Size: 399 229 102 10 80 195 63 270 Cuts at: 2739 2823 2927 3669 3754 4825 1391 Size: 84 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 270 229 195 104 102 85 84 80 63 10 BspEI T'CCGG_A Cuts at: 2011 5271 2011 Size: 3260 2348 BspHI T'CATG_A Cuts at: 2418 3497 5227 2418 Size: 1079 1730 2799 Fragments arranged by size: 2799 1730 1079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2438 2963 844 Size: 1594 525 3489 Fragments arranged by size: 3489 1594 525 BsrBI CCG'CTC Cuts at: 1394 1823 2781 3495 1394 Size: 429 958 714 3507 Fragments arranged by size: 3507 958 714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 4103 4277 65 Size: 1183 1071 1784 174 1396 Fragments arranged by size: 1784 1396 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 794 Size: 1421 4187 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2731 2392 Size: 339 5269 BstYI r'GATC_y Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603 Size: 966 367 385 17 1452 86 12 346 Cuts at: 5603 1972 Size: 1977 Fragments arranged by size: 1977 1452 966 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2304 513 Size: 552 1239 3817 Fragments arranged by size: 3817 1239 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2350 2698 487 Size: 683 56 1124 348 3397 Fragments arranged by size: 3397 1124 683 348 56 BtsI GCAGTG_nn' Cuts at: 3124 3918 3938 3124 Size: 794 20 4794 Fragments arranged by size: 4794 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 3055 3944 Size: 54 1425 50 275 18 343 882 889 Cuts at: 3944 5573 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 882 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2567 3538 1353 Size: 1214 971 3423 Fragments arranged by size: 3423 1214 971 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2137 2849 4195 4569 4715 387 Size: 1064 138 548 712 1346 374 146 1280 Fragments arranged by size: 1346 1280 1064 712 548 374 146 138 EcoICRI GAG'CTC Cuts at: 2821 2821 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2356 3671 4268 5055 5323 1239 Size: 393 622 102 1315 597 787 268 1524 Fragments arranged by size: 1524 1315 787 622 597 393 268 102 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2570 Size: 5608 EcoO109I rG'GnC_Cy Cuts at: 2127 2128 2127 Size: 1 5607 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2225 4755 1678 Size: 547 2530 2531 Fragments arranged by size: 2531 2530 547 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 4249 4281 4323 Size: 32 135 32 697 32 1948 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 1948 697 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 2258 748 Size: 1510 4098 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2729 2732 3566 3925 4084 518 Size: 648 1221 342 3 834 359 159 2042 Fragments arranged by size: 2042 1221 834 648 359 342 159 3 MspA1I CmG'CkG Cuts at: 1606 2079 2106 2329 2685 2708 2860 3024 3701 Size: 473 27 223 356 23 152 164 677 Cuts at: 3701 4849 5094 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 473 356 245 223 164 152 27 23 NcoI C'CATG_G Cuts at: 513 1065 2304 513 Size: 552 1239 3817 Fragments arranged by size: 3817 1239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2509 2966 4511 1132 Size: 97 1280 457 1545 2229 Fragments arranged by size: 2229 1545 1280 457 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2127 2127 Size: 5608 PstI C_TGCA'G Cuts at: 830 2960 830 Size: 2130 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2329 2708 2860 2329 Size: 379 152 5077 Fragments arranged by size: 5077 379 152 SacI G_AGCT'C Cuts at: 2823 2823 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SapI GCTCTTCn'nnn_ Cuts at: 2567 2567 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2956 4091 4772 4963 826 Size: 219 1911 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2124 2697 2935 1872 Size: 252 573 238 4545 Fragments arranged by size: 4545 573 252 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 2245 2609 3712 4613 4875 820 Size: 197 926 302 364 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 364 302 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2509 2966 1132 Size: 1377 457 3774 Fragments arranged by size: 3774 1377 457 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2304 2630 3047 513 Size: 552 1239 326 417 3074 Fragments arranged by size: 3074 1239 552 417 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 2816 2820 3635 3652 3805 3990 Size: 1255 890 346 4 815 17 153 185 Cuts at: 3990 4435 325 Size: 445 1498 Fragments arranged by size: 1498 1255 890 815 445 346 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2392 2311 Size: 81 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2122 2695 2933 1870 Size: 252 573 238 4545 Fragments arranged by size: 4545 573 252 238 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI