################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AhdI AlwI AlwNI AocI ApoI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MunI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI Sau3AI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 485 GGCGCC G[GA]^CG[CT]C AflIII 791 ACGTGT A^C[GA][CT]GT Alw44I 1021 GTGCAC G^TGCAC Aor51HI 350 AGCGCT AGC^GCT ApaLI 1021 GTGCAC G^TGCAC AspI 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 995 TGGCCA TGG^CCA BanI 485 GGCGCC G^G[CT][GA]CC BbeI 485 GGCGCC GGCGC^C BbvCI 1173 CCTCAGC CCTCAGC(-5/-2) BlpI 1161 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1161 GCTCAGC GC^T[ACGT]AGC BsaHI 485 GGCGCC G[GA]^CG[CT]C BsaI 667 GAGACC GGTCTC(1/5) BsmAI 667 GAGAC GTCTC(1/5) BsrBI 622 GAGCGG CCGCTC(-3/-3) BstAPI 221 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1203 GGTCACC G^GT[ACGT]ACC BstPI 1203 GGTCACC G^GT[ACGT]ACC BstXI 833 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1161 GCTCAGC GC^T[ACGT]AGC Eco47III 350 AGCGCT AGC^GCT EcoNI 1287 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1203 GGTCACC G^GT[ACGT]ACC Hin1I 485 GGCGCC G[GA]^CG[CT]C HinfI 1366 GACTC G^A[ACGT]TC HphI 1205 TCACC GGTGA(8/7) KasI 485 GGCGCC G^GCGCC MluNI 995 TGGCCA TGG^CCA MscI 995 TGGCCA TGG^CCA NarI 485 GGCGCC GG^CGCC PflFI 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1366 GACTC GAGTC(4/5) SacI 1243 GAGCTC GAGCT^C SfiI 564 GGCCGTCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 485 GGCGCC GGC^GCC SmlI 1246 CTCAAG C^T[CT][GA]AG TspRI 1016 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1100 CCATGACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### ApaI 285 GGGCCC GGGCC^C 1338 GGGCCC AscI 339 GGCGCGCC GG^CGCGCC 906 GGCGCGCC BcgI 530 GCAAGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 650 GCAGCCTGTTCG BsaAI 790 TACGTG [CT]AC^GT[GA] 892 TACGTG BspMI 164 ACCTGC ACCTGC(4/8) 1149 GCAGGT BsrFI 479 GCCGGC [GA]^CCGG[CT] 1281 GCCGGC BsrI 323 ACTGG ACTGG(1/-1) 1017 ACTGG Cfr10I 479 GCCGGC [GA]^CCGG[CT] 1281 GCCGGC EarI 535 CTCTTC CTCTTC(1/4) 1006 CTCTTC EcoT14I 7 CCATGG C^C[AT][AT]GG 998 CCATGG Ksp632I 535 CTCTTC CTCTTC(1/4) 1006 CTCTTC KspI 150 CCGCGG CCGC^GG 1370 CCGCGG NaeI 479 GCCGGC GCC^GGC 1281 GCCGGC NcoI 7 CCATGG C^CATGG 998 CCATGG NgoMIV 479 GCCGGC G^CCGGC 1281 GCCGGC PflMI 912 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1076 CCAGCTATTGG PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT] 453 GGGACCT SacII 150 CCGCGG CCGC^GG 1370 CCGCGG SapI 534 GCTCTTC GCTCTTC(1/4) 1005 GCTCTTC SbfI 714 CCTGCAGG CCTGCA^GG 1287 CCTGCAGG Sse8387I 714 CCTGCAGG CCTGCA^GG 1287 CCTGCAGG StyI 7 CCATGG C^C[AT][AT]GG 998 CCATGG Van91I 912 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1076 CCAGCTATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AluI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TthHB8I XmaCI XmaI