################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI MaeI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 865 GACGTC GACGT^C AccIII 137 TCCGGA T^CCGGA AcsI 235 AAATTC [GA]^AATT[CT] AflIII 458 ACGTGT A^C[GA][CT]GT AhdI 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 235 AAATTC [GA]^AATT[CT] AspEI 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 888 GTGCTC G[AT]GC[AT]^C AspI 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 85 CTCGAG C^[CT]CG[GA]G AviII 446 TGCGCA TGC^GCA BcnI 870 CCCGG CC^[GC]GG BglI 840 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1007 AGATCT A^GATCT BpmI 184 CTGGAG CTGGAG(16/14) BsaBI 135 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 137 TCCGGA [AT]^CCGG[AT] BseAI 137 TCCGGA T^CCGGA BsiHKAI 888 GTGCTC G[AT]GC[AT]^C BsoBI 85 CTCGAG C^[CT]CG[GA]G BspEI 137 TCCGGA T^CCGGA BssSI 97 CACGAG CACGAG(-5/-1) BstAPI 9 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 706 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 835 CCGCGG C^C[GA][CT]GG Eam1105I 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 706 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 629 CCAAGG C^C[AT][AT]GG FspI 446 TGCGCA TGC^GCA HincII 588 GTTGAC GT[CT]^[GA]AC HindII 588 GTTGAC GT[CT]^[GA]AC HindIII 961 AAGCTT A^AGCTT KspI 835 CCGCGG CCGC^GG MamI 135 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 137 TCCGGA T^CCGGA NciI 870 CCCGG CC^[GC]GG PaeR7I 85 CTCGAG C^TCGAG PflFI 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 360 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 181 CAGCTG CAG^CTG SacII 835 CCGCGG CCGC^GG SexAI 719 ACCTGGT A^CC[AT]GGT SfiI 839 GGCCCAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 219 CGCCGGCG C[GA]^CCGG[CT]G SmlI 85 CTCGAG C^T[CT][GA]AG SphI 443 GCATGC GCATG^C StuI 320 AGGCCT AGG^CCT StyI 629 CCAAGG C^C[AT][AT]GG Tsp45I 478 GTGAC ^GT[GC]AC Tsp509I 236 AATT ^AATT Tth111I 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 85 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 153 GTAC GT^AC 855 GTAC AlwNI 376 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1093 CAGAGGCTG AocI 729 CCTCAGG CC^T[ACGT]AGG 826 CCTGAGG Asp700I 396 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 945 GAACTCCTTC AvaII 93 GGACC G^G[AT]CC 873 GGACC BbeI 64 GGCGCC GGCGC^C 634 GGCGCC BmyI 289 GGGCAC G[AGT]GC[ACT]^C 888 GTGCTC BsaAI 613 TACGTG [CT]AC^GT[GA] 658 TACGTG BsiEI 524 CGGCCG CG[GA][CT]^CG 924 CGGCCG Bsp1286I 289 GGGCAC G[AGT]GC[ACT]^C 888 GTGCTC BstYI 134 AGATCC [GA]^GATC[CT] 1007 AGATCT Bsu36I 729 CCTCAGG CC^T[ACGT]AGG 826 CCTGAGG DdeI 730 CTCAG C^T[ACGT]AG 827 CTGAG EaeI 524 CGGCCG [CT]^GGCC[GA] 924 CGGCCG EagI 524 CGGCCG C^GGCCG 924 CGGCCG EclXI 524 CGGCCG C^GGCCG 924 CGGCCG Eco52I 524 CGGCCG C^GGCCG 924 CGGCCG Eco81I 729 CCTCAGG CC^T[ACGT]AGG 826 CCTGAGG HinfI 21 GACTC G^A[ACGT]TC 88 GAGTC KasI 64 GGCGCC G^GCGCC 634 GGCGCC MaeIII 478 GTGAC ^GT[ACGT]AC 1071 GTTAC MflI 134 AGATCC [GA]^GATC[CT] 1007 AGATCT MseI 428 TTAA T^TAA 1024 TTAA MslI 399 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 453 CACTGACGTG NarI 64 GGCGCC GG^CGCC 634 GGCGCC NotI 523 GCGGCCGC GC^GGCCGC 923 GCGGCCGC NspI 329 GCATGT [GA]CATG^[CT] 443 GCATGC PleI 21 GACTC GAGTC(4/5) 88 GAGTC RsaI 153 GTAC GT^AC 855 GTAC SfoI 64 GGCGCC GGC^GCC 634 GGCGCC Tru9I 428 TTAA T^TAA 1024 TTAA XhoII 134 AGATCC [GA]^GATC[CT] 1007 AGATCT XmnI 396 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 945 GAACTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AluI AlwI BanI BbvI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NdeII NgoMIV NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I