Restriction map for
pFN21AE5020
(Circular) (Six-base) MAPSORT of: pFN21AE5020.seq Check: 9495 from: 1 to: 5827
pFN21AE5020 5827 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2912 2912
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2874 3637 278
Size: 53 83 186 936 1338 763 2468
Fragments arranged by size:
2468 1338 936 763 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2799 2941 3890 5274 5542 1632
Size: 812 355 142 949 1384 268 1917
Fragments arranged by size:
1917 1384 949 812 355 268 142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2463 4726 5703 1203
Size: 1260 2263 977 1327
Fragments arranged by size:
2263 1327 1260 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 2884 4556 2884
Size: 1672 4155
AleI CACnn'nnGTG
Cuts at: 1166 2408 2462 1166
Size: 1242 54 4531
Fragments arranged by size:
4531 1242 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2386 3103 3570 5142 2386
Size: 717 467 1572 3071
Fragments arranged by size:
3071 1572 717 467
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2240 3136 3362 3514 5623 1493
Size: 747 896 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3152 1870
Size: 73 147 1062 4545
Fragments arranged by size:
4545 1062 147 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2309 2639 2941 3148
Size: 325 217 627 282 240 330 302 207
Cuts at: 3148 4603 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 330 325 302 282 240
217 207
BanII G_rGCy'C
Cuts at: 1391 3146 1391
Size: 1755 4072
BbeI G_GCGC'C
Cuts at: 2073 2643 2073
Size: 570 5257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2851 4438 136
Size: 107 122 71 631 605 1179 1587 1525
Fragments arranged by size:
1587 1525 1179 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3012 5822 3012
Size: 2810 3017
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2298 3888 5044 1790
Size: 508 1590 1156 2573
Fragments arranged by size:
2573 1590 1156 508
BmgBI CAC'GTC
Cuts at: 1318 2464 1318
Size: 1146 4681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2536 3558 4516
Size: 416 53 83 399 24 1119 1022 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1119 1022 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 4487 1239
Size: 971 2277 2579
Fragments arranged by size:
2579 2277 971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2620 2665 3618 493
Size: 711 1416 45 953 2702
Fragments arranged by size:
2702 1416 953 711 45
BsaBI GATnn'nnATC
Cuts at: 2144 5821 2144
Size: 3677 2150
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2085 2229 2640
Size: 53 83 186 936 537 15 144 411
Cuts at: 2640 2871 3634 4016 4651 275
Size: 231 763 382 635 1451
Fragments arranged by size:
1451 936 763 635 537 411 382 231
186 144 83 53 15
BsaWI w'CCGG_w
Cuts at: 2142 4260 4658 4932 5079 5490 2142
Size: 2118 398 274 147 411 2479
Fragments arranged by size:
2479 2118 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2972 3002 1178
Size: 30 165 30 1569 30 4003
Fragments arranged by size:
4003 1569 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2132 2150 2564 2849 3023 1186
Size: 943 3 18 414 285 174 3990
Fragments arranged by size:
3990 943 414 285 174 18 3
BseYI C'CCAG_C
Cuts at: 2350 2846 5030 5526 2350
Size: 496 2184 496 2651
Fragments arranged by size:
2651 2184 496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2286 2884 1163
Size: 573 293 257 598 4106
Fragments arranged by size:
4106 598 573 293 257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2532 2932 3277 4038 5066 1540
Size: 275 186 531 400 345 761 1028 2301
Fragments arranged by size:
2301 1028 761 531 400 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 2897 3888 3973 5044 2897
Size: 991 85 1071 3680
Fragments arranged by size:
3680 1071 991 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2897 3146 3888 3973 5044 1391
Size: 399 508 599 249 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 599 508 399 249 85
BspEI T'CCGG_A
Cuts at: 2142 5490 2142
Size: 3348 2479
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2427 2889 2912 3182 844
Size: 1223 360 462 23 270 3489
Fragments arranged by size:
3489 1223 462 360 270 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2659 2767 2913 2934 3714 1394
Size: 429 836 108 146 21 780 3507
Fragments arranged by size:
3507 836 780 429 146 108 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 2225 2246 2850 4471 4658 1700
Size: 110 371 44 21 604 1621 187 2869
Fragments arranged by size:
2869 1621 604 371 187 110 44 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 2102 3887 4899 2102
Size: 1785 1012 3030
Fragments arranged by size:
3030 1785 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4601
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2139 3012 3157 3524 3909 3926 5378 5464
Size: 167 873 145 367 385 17 1452 86
Cuts at: 5464 5476 5822 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 873 385 367 346 167 145
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2735 2832 2735
Size: 97 5730
BtgI C'CryG_G
Cuts at: 513 1065 2840 513
Size: 552 1775 3500
Fragments arranged by size:
3500 1775 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3033 487
Size: 683 56 1807 3281
Fragments arranged by size:
3281 1807 683 56
BtsI GCAGTG_nn'
Cuts at: 3343 4137 4157 3343
Size: 794 20 5013
Fragments arranged by size:
5013 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 2929 3274
Size: 54 1425 50 275 18 699 400 345
Cuts at: 3274 4163 5792 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 699 400 345 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2529 2929 3274 1537
Size: 275 717 400 345 4090
Fragments arranged by size:
4090 717 400 345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2775 3047 3757 1353
Size: 650 772 272 710 3423
Fragments arranged by size:
3423 772 710 650 272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2882 4414 4788 4934 387
Size: 1064 138 1293 1532 374 146 1280
Fragments arranged by size:
1532 1293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2444 2799 2941 3890 4487 5274
Size: 393 578 234 355 142 949 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 949 787 597 578 393 355 268
234 142
EcoO109I rG'GnC_Cy
Cuts at: 2712 2712
Size: 5827
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 2453 4333 2453
Size: 1880 3947
HaeII r_GCGC'y
Cuts at: 1678 2073 2643 2964 4974 1678
Size: 395 570 321 2010 2531
Fragments arranged by size:
2531 2010 570 395 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2774 2806 2870 2902 4468
Size: 32 135 32 1202 32 64 32 1566
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 1202 135 64 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2595 3171 3417 669
Size: 988 281 657 576 246 3079
Fragments arranged by size:
3079 988 657 576 281 246
HindIII A'AGCT_T
Cuts at: 748 2966 748
Size: 2218 3609
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2069 2639 2069
Size: 570 5257
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 2190 4941 5125 1305
Size: 771 114 2751 184 2007
Fragments arranged by size:
2751 2007 771 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2462 3785 4144 4303 518
Size: 648 1242 54 1323 359 159 2042
Fragments arranged by size:
2042 1323 1242 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2188 2362 2842 3243 3920 5068 5313 1606
Size: 582 174 480 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 480 401 245 174
NaeI GCC'GGC
Cuts at: 2227 2248 2852 2227
Size: 21 604 5202
Fragments arranged by size:
5202 604 21
NarI GG'CG_CC
Cuts at: 2070 2640 2070
Size: 570 5257
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2225 2246 2850 2225
Size: 21 604 5202
Fragments arranged by size:
5202 604 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 2529 2929 3274 2529
Size: 400 345 5082
Fragments arranged by size:
5082 400 345
NspI r_CATG'y
Cuts at: 1132 1229 2338 2452 3185 4730 1132
Size: 97 1109 114 733 1545 2229
Fragments arranged by size:
2229 1545 1109 733 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4549
PfoI T'CCnGG_A
Cuts at: 2874 2874
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2369 1613
Size: 756 5071
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspXI vC'TCGA_Gb
Cuts at: 2090 2090
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2208 2631 2649 2886 3179 830
Size: 1378 423 18 237 293 3478
Fragments arranged by size:
3478 1378 423 293 237 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 5827
SacII CC_GC'GG
Cuts at: 2843 2843
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SexAI A'CCwGG_T
Cuts at: 2724 2724
Size: 5827
SfcI C'TryA_G
Cuts at: 826 1045 2204 2627 2645 2882 3175 4310 4991
Size: 219 1159 423 18 237 293 1135 681
Cuts at: 4991 5182 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 681 423 293 237 219
191 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2851 2851
Size: 5827
SfoI GGC'GCC
Cuts at: 2071 2641 2071
Size: 570 5257
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 2225 1700
Size: 525 5302
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3931 4832 5094 820
Size: 197 926 147 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 2452 3185 1132
Size: 1320 733 3774
Fragments arranged by size:
3774 1320 733
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2327 1517
Size: 810 5017
StyI C'CwwG_G
Cuts at: 513 1065 2634 3266 513
Size: 552 1569 632 3074
Fragments arranged by size:
3074 1569 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 4073 5785
Size: 274 80 33 51 1443 180 1916 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1916 1712 1443 274 180 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 2029 1316
Size: 713 5114
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 2090 1943
Size: 147 5680
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 2405 2954 3956 2405
Size: 549 1002 4276
Fragments arranged by size:
4276 1002 549
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2872 3635 276
Size: 53 83 186 936 1338 763 2468
Fragments arranged by size:
2468 1338 936 763 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PsrI RsrII SacI SanDI SapI SrfI
SwaI XcmI