(Linear) (Six-base) MAPSORT of: KIEE5020.seq Check: 6412 from: 1 to: 1122 KIEE5020.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 907 1122 Size: 907 215 AatII G_ACGT'C Cuts at: 0 869 1122 Size: 869 253 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 794 936 1122 Size: 439 355 142 186 Fragments arranged by size: 439 355 186 142 AflIII A'CryG_T Cuts at: 0 458 1122 Size: 458 664 AhdI GACnn_n'nnGTC Cuts at: 0 879 1122 Size: 879 243 AleI CACnn'nnGTG Cuts at: 0 403 457 1122 Size: 403 54 665 Fragments arranged by size: 665 403 54 AlwNI CAG_nnn'CTG Cuts at: 0 381 1098 1122 Size: 381 717 24 Fragments arranged by size: 717 381 24 ApoI r'AATT_y Cuts at: 0 235 1122 Size: 235 887 AvaI C'yCGr_G Cuts at: 0 85 1122 Size: 85 1037 BanI G'GyrC_C Cuts at: 0 64 304 634 936 1122 Size: 64 240 330 302 186 Fragments arranged by size: 330 302 240 186 64 BbeI G_GCGC'C Cuts at: 0 68 638 1122 Size: 68 570 484 Fragments arranged by size: 570 484 68 BglI GCCn_nnn'nGGC Cuts at: 0 846 1122 Size: 846 276 BglII A'GATC_T Cuts at: 0 1007 1122 Size: 1007 115 Bme1580I G_kGCm'C Cuts at: 0 293 1122 Size: 293 829 BmgBI CAC'GTC Cuts at: 0 459 1122 Size: 459 663 BmrI ACTGGGnnnn_n' Cuts at: 0 531 1122 Size: 531 591 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1026 1122 Size: 1026 96 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 1122 Size: 205 917 BsaAI yAC'GTr Cuts at: 0 615 660 1122 Size: 615 45 462 Fragments arranged by size: 615 462 45 BsaBI GATnn'nnATC Cuts at: 0 139 1122 Size: 139 983 BsaHI Gr'CG_yC Cuts at: 0 65 80 224 635 866 1122 Size: 65 15 144 411 231 256 Fragments arranged by size: 411 256 231 144 65 15 BsaWI w'CCGG_w Cuts at: 0 137 1122 Size: 137 985 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 967 997 1122 Size: 967 30 125 Fragments arranged by size: 967 125 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 127 145 559 844 1018 1122 Size: 124 3 18 414 285 174 104 Fragments arranged by size: 414 285 174 124 104 18 3 BseYI C'CCAG_C Cuts at: 0 345 841 1122 Size: 345 496 281 Fragments arranged by size: 496 345 281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 281 879 1122 Size: 24 257 598 243 Fragments arranged by size: 598 257 243 24 BsiEI CG_ry'CG Cuts at: 0 527 927 1122 Size: 527 400 195 Fragments arranged by size: 527 400 195 BsiHKAI G_wGCw'C Cuts at: 0 892 1122 Size: 892 230 Bsp1286I G_dGCh'C Cuts at: 0 293 892 1122 Size: 293 599 230 Fragments arranged by size: 599 293 230 BspEI T'CCGG_A Cuts at: 0 137 1122 Size: 137 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 62 422 884 907 1122 Size: 62 360 462 23 215 Fragments arranged by size: 462 360 215 62 23 BsrBI CCG'CTC Cuts at: 0 654 762 908 929 1122 Size: 654 108 146 21 193 Fragments arranged by size: 654 193 146 108 21 BsrFI r'CCGG_y Cuts at: 0 176 220 241 845 1122 Size: 176 44 21 604 277 Fragments arranged by size: 604 277 176 44 21 BssSI C'ACGA_G Cuts at: 0 97 1122 Size: 97 1025 BstAPI GCAn_nnn'nTGC Cuts at: 0 15 1122 Size: 15 1107 BstYI r'GATC_y Cuts at: 0 134 1007 1122 Size: 134 873 115 Fragments arranged by size: 873 134 115 Bsu36I CC'TnA_GG Cuts at: 0 730 827 1122 Size: 730 97 295 Fragments arranged by size: 730 295 97 BtgI C'CryG_G Cuts at: 0 835 1122 Size: 835 287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1028 1122 Size: 1028 94 EaeI y'GGCC_r Cuts at: 0 524 924 1122 Size: 524 400 198 Fragments arranged by size: 524 400 198 EagI C'GGCC_G Cuts at: 0 524 924 1122 Size: 524 400 198 Fragments arranged by size: 524 400 198 EarI CTCTTCn'nnn_ Cuts at: 0 770 1042 1122 Size: 770 272 80 Fragments arranged by size: 770 272 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 877 1122 Size: 877 245 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 439 794 936 1122 Size: 205 234 355 142 186 Fragments arranged by size: 355 234 205 186 142 EcoO109I rG'GnC_Cy Cuts at: 0 707 1122 Size: 707 415 FspI TGC'GCA Cuts at: 0 448 1122 Size: 448 674 HaeII r_GCGC'y Cuts at: 0 68 638 959 1122 Size: 68 570 321 163 Fragments arranged by size: 570 321 163 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 769 801 865 897 1122 Size: 769 32 64 32 225 Fragments arranged by size: 769 225 64 32 32 HincII GTy'rAC Cuts at: 0 590 1122 Size: 590 532 HindIII A'AGCT_T Cuts at: 0 961 1122 Size: 961 161 KasI G'GCGC_C Cuts at: 0 64 634 1122 Size: 64 570 488 Fragments arranged by size: 570 488 64 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 71 185 1122 Size: 71 114 937 Fragments arranged by size: 937 114 71 MslI CAynn'nnrTG Cuts at: 0 403 457 1122 Size: 403 54 665 Fragments arranged by size: 665 403 54 MspA1I CmG'CkG Cuts at: 0 183 357 837 1122 Size: 183 174 480 285 Fragments arranged by size: 480 285 183 174 NaeI GCC'GGC Cuts at: 0 222 243 847 1122 Size: 222 21 604 275 Fragments arranged by size: 604 275 222 21 NarI GG'CG_CC Cuts at: 0 65 635 1122 Size: 65 570 487 Fragments arranged by size: 570 487 65 NgoMIV G'CCGG_C Cuts at: 0 220 241 845 1122 Size: 220 21 604 277 Fragments arranged by size: 604 277 220 21 NotI GC'GGCC_GC Cuts at: 0 524 924 1122 Size: 524 400 198 Fragments arranged by size: 524 400 198 NspI r_CATG'y Cuts at: 0 333 447 1122 Size: 333 114 675 Fragments arranged by size: 675 333 114 PflMI CCAn_nnn'nTGG Cuts at: 0 381 1122 Size: 381 741 PfoI T'CCnGG_A Cuts at: 0 869 1122 Size: 869 253 PshAI GACnn'nnGTC Cuts at: 0 364 1122 Size: 364 758 PspXI vC'TCGA_Gb Cuts at: 0 85 1122 Size: 85 1037 PstI C_TGCA'G Cuts at: 0 203 626 644 881 1122 Size: 203 423 18 237 241 Fragments arranged by size: 423 241 237 203 18 PvuII CAG'CTG Cuts at: 0 183 1122 Size: 183 939 SacII CC_GC'GG Cuts at: 0 838 1122 Size: 838 284 SexAI A'CCwGG_T Cuts at: 0 719 1122 Size: 719 403 SfcI C'TryA_G Cuts at: 0 199 622 640 877 1122 Size: 199 423 18 237 245 Fragments arranged by size: 423 245 237 199 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 846 1122 Size: 846 276 SfoI GGC'GCC Cuts at: 0 66 636 1122 Size: 66 570 486 Fragments arranged by size: 570 486 66 SgrAI Cr'CCGG_yG Cuts at: 0 220 1122 Size: 220 902 SmlI C'TyrA_G Cuts at: 0 85 1122 Size: 85 1037 SphI G_CATG'C Cuts at: 0 447 1122 Size: 447 675 StuI AGG'CCT Cuts at: 0 322 1122 Size: 322 800 StyI C'CwwG_G Cuts at: 0 629 1122 Size: 629 493 TatI w'GTAC_w Cuts at: 0 152 1122 Size: 152 970 Tth111I GACn'n_nGTC Cuts at: 0 24 1122 Size: 24 1098 XhoI C'TCGA_G Cuts at: 0 85 1122 Size: 85 1037 XmnI GAAnn'nnTTC Cuts at: 0 400 949 1122 Size: 400 549 173 Fragments arranged by size: 549 400 173 ZraI GAC'GTC Cuts at: 0 867 1122 Size: 867 255 Enzymes that do cut: AarI AatII AcuI AflIII AhdI AleI AlwNI ApoI AvaI BanI BbeI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI PfoI PshAI PspXI PstI PvuII SacII SexAI SfcI SfiI SfoI SgrAI SmlI SphI StuI StyI TatI Tth111I XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI Bpu10I BpuEI BsaI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TstI XbaI XcmI XmaI