(Linear) (Six-base) MAPSORT of: KIEE5653.seq Check: 9382 from: 1 to: 1518 KIEE5653.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. Acc65I G'GTAC_C Cuts at: 0 231 1518 Size: 231 1287 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 1518 Size: 817 701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 315 545 1482 1518 Size: 315 230 937 36 Fragments arranged by size: 937 315 230 36 AlwNI CAG_nnn'CTG Cuts at: 0 1096 1518 Size: 1096 422 ApaLI G'TGCA_C Cuts at: 0 73 371 1518 Size: 73 298 1147 Fragments arranged by size: 1147 298 73 ApoI r'AATT_y Cuts at: 0 28 58 805 1518 Size: 28 30 747 713 Fragments arranged by size: 747 713 30 28 AvaI C'yCGr_G Cuts at: 0 557 1518 Size: 557 961 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 721 754 1518 Size: 721 33 764 Fragments arranged by size: 764 721 33 BanI G'GyrC_C Cuts at: 0 231 1518 Size: 231 1287 BanII G_rGCy'C Cuts at: 0 228 558 1403 1518 Size: 228 330 845 115 Fragments arranged by size: 845 330 228 115 BbsI GAAGACnn'nnnn_ Cuts at: 0 782 1518 Size: 782 736 BbvCI CC'TCA_GC Cuts at: 0 518 1208 1214 1518 Size: 518 690 6 304 Fragments arranged by size: 690 518 304 6 Bme1580I G_kGCm'C Cuts at: 0 77 375 1387 1518 Size: 77 298 1012 131 Fragments arranged by size: 1012 298 131 77 BmtI G_CTAG'C Cuts at: 0 359 1379 1518 Size: 359 1020 139 Fragments arranged by size: 1020 359 139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 953 1518 Size: 196 757 565 Fragments arranged by size: 757 565 196 Bpu10I CC'TnA_GC Cuts at: 0 518 1208 1214 1518 Size: 518 690 6 304 Fragments arranged by size: 690 518 304 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 1518 Size: 627 891 BsaI GGTCTCn'nnnn_ Cuts at: 0 1195 1518 Size: 1195 323 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 215 245 1518 Size: 215 30 1273 Fragments arranged by size: 1273 215 30 BseYI C'CCAG_C Cuts at: 0 1029 1518 Size: 1029 489 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 691 1466 1518 Size: 28 663 775 52 Fragments arranged by size: 775 663 52 28 BsiHKAI G_wGCw'C Cuts at: 0 77 375 1403 1518 Size: 77 298 1028 115 Fragments arranged by size: 1028 298 115 77 Bsp1286I G_dGCh'C Cuts at: 0 77 228 375 558 1387 1403 1518 Size: 77 151 147 183 829 16 115 Fragments arranged by size: 829 183 151 147 115 77 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 127 1518 Size: 127 1391 BsrDI GCAATG_nn' Cuts at: 0 1136 1518 Size: 1136 382 BsrGI T'GTAC_A Cuts at: 0 1291 1389 1518 Size: 1291 98 129 Fragments arranged by size: 1291 129 98 BssSI C'ACGA_G Cuts at: 0 1277 1518 Size: 1277 241 BstAPI GCAn_nnn'nTGC Cuts at: 0 119 1518 Size: 119 1399 BstEII G'GTnAC_C Cuts at: 0 759 1024 1518 Size: 759 265 494 Fragments arranged by size: 759 494 265 BstXI CCAn_nnnn'nTGG Cuts at: 0 220 1518 Size: 220 1298 Bsu36I CC'TnA_GG Cuts at: 0 695 1518 Size: 695 823 BtgI C'CryG_G Cuts at: 0 863 1335 1518 Size: 863 472 183 Fragments arranged by size: 863 472 183 BtsI GCAGTG_nn' Cuts at: 0 1454 1518 Size: 1454 64 DraIII CAC_nnn'GTG Cuts at: 0 337 1518 Size: 337 1181 EarI CTCTTCn'nnn_ Cuts at: 0 587 786 1307 1479 1505 1518 Size: 587 199 521 172 26 13 Fragments arranged by size: 587 521 199 172 26 13 EcoICRI GAG'CTC Cuts at: 0 1401 1518 Size: 1401 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 817 953 1518 Size: 196 621 136 565 Fragments arranged by size: 621 565 196 136 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 138 1518 Size: 138 1380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 261 763 795 1518 Size: 229 32 502 32 723 Fragments arranged by size: 723 502 229 32 32 HincII GTy'rAC Cuts at: 0 198 421 1518 Size: 198 223 1097 Fragments arranged by size: 1097 223 198 HindIII A'AGCT_T Cuts at: 0 143 1518 Size: 143 1375 KpnI G_GTAC'C Cuts at: 0 235 1518 Size: 235 1283 MfeI C'AATT_G Cuts at: 0 1004 1518 Size: 1004 514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 201 1518 Size: 201 1317 MspA1I CmG'CkG Cuts at: 0 547 795 1119 1518 Size: 547 248 324 399 Fragments arranged by size: 547 399 324 248 NcoI C'CATG_G Cuts at: 0 863 1518 Size: 863 655 NheI G'CTAG_C Cuts at: 0 355 1375 1518 Size: 355 1020 143 Fragments arranged by size: 1020 355 143 NspI r_CATG'y Cuts at: 0 942 1245 1469 1518 Size: 942 303 224 49 Fragments arranged by size: 942 303 224 49 PflMI CCAn_nnn'nTGG Cuts at: 0 337 539 1518 Size: 337 202 979 Fragments arranged by size: 979 337 202 PfoI T'CCnGG_A Cuts at: 0 992 1518 Size: 992 526 PspXI vC'TCGA_Gb Cuts at: 0 557 1518 Size: 557 961 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 485 517 1275 1307 1518 Size: 485 32 758 32 211 Fragments arranged by size: 758 485 211 32 32 PvuII CAG'CTG Cuts at: 0 795 1119 1518 Size: 795 324 399 Fragments arranged by size: 795 399 324 SacI G_AGCT'C Cuts at: 0 1403 1518 Size: 1403 115 SapI GCTCTTCn'nnn_ Cuts at: 0 1307 1518 Size: 1307 211 SfcI C'TryA_G Cuts at: 0 961 1518 Size: 961 557 SmlI C'TyrA_G Cuts at: 0 557 642 1518 Size: 557 85 876 Fragments arranged by size: 876 557 85 StuI AGG'CCT Cuts at: 0 598 1053 1518 Size: 598 455 465 Fragments arranged by size: 598 465 455 StyI C'CwwG_G Cuts at: 0 471 863 1518 Size: 471 392 655 Fragments arranged by size: 655 471 392 TatI w'GTAC_w Cuts at: 0 186 1291 1389 1518 Size: 186 1105 98 129 Fragments arranged by size: 1105 186 129 98 XhoI C'TCGA_G Cuts at: 0 557 1518 Size: 557 961 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI Bsu36I BtgI BtsI DraIII EarI EcoICRI Eco57MI FalI Hin4I HincII HindIII KpnI MfeI MmeI MspA1I NcoI NheI NspI PflMI PfoI PspXI PsrI PvuII SacI SapI SfcI SmlI StuI StyI TatI XhoI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI MluI MscI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XmaI XmnI ZraI