################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII BamHI BanI BanII BbeI BbrPI BcgI BclI BcnI BfrI BglI BglII BlpI BmyI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 485 GTATAC GT^[AC][GT]AC AcsI 98 GAATTT [GA]^AATT[CT] AfaI 177 GTAC GT^AC ApoI 98 GAATTT [GA]^AATT[CT] AspI 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 677 TGCGCA TGC^GCA AvrII 412 CCTAGG C^CTAGG BbsI 442 GTCTTC GAAGAC(2/6) BbvCI 126 CCTCAGC CCTCAGC(-5/-2) BciVI 817 GTATCC GTATCC(6/5) BlnI 412 CCTAGG C^CTAGG BpuAI 442 GTCTTC GAAGAC(2/6) BsaAI 367 TACGTG [CT]AC^GT[GA] BsaI 758 GGTCTC GGTCTC(1/5) BsiEI 360 CGATCG CG[GA][CT]^CG BsmAI 759 GTCTC GTCTC(1/5) BsmI 331 GAATGC GAATGC(1/-1) Bst1107I 485 GTATAC GTA^TAC BstYI 268 AGATCC [GA]^GATC[CT] BstZ17I 485 GTATAC GTA^TAC CfoI 678 GCGC GCG^C EcoT22I 288 ATGCAT ATGCA^T FspI 677 TGCGCA TGC^GCA HhaI 678 GCGC GCG^C HinP1I 678 GCGC G^CGC HincII 158 GTCAAC GT[CT]^[GA]AC HindII 158 GTCAAC GT[CT]^[GA]AC MaeII 368 ACGT A^CGT MflI 268 AGATCC [GA]^GATC[CT] NdeI 650 CATATG CA^TATG NsiI 288 ATGCAT ATGCA^T NspI 338 ACATGC [GA]CATG^[CT] PflFI 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 378 CTGCAG CTGCA^G PvuI 360 CGATCG CGAT^CG RsaI 177 GTAC GT^AC Tth111I 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 268 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 23 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 107 CAGTTACTG BalI 371 TGGCCA TGG^CCA 623 TGGCCA BfaI 413 CTAG C^TAG 791 CTAG BpmI 567 CTCCAG CTGGAG(16/14) 935 CTCCAG BsmFI 408 GTCCC GGGAC(10/14) 544 GTCCC BspMI 783 ACCTGC ACCTGC(4/8) 698 GCAGGT BssKI 807 CCAGG ^CC[ACGT]GG 937 CCAGG BstNI 807 CCAGG CC^[AT]GG 937 CCAGG Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC 355 GCCTGC DsaI 350 CCATGG C^C[GA][CT]GG 474 CCATGG EaeI 371 TGGCCA [CT]^GGCC[GA] 623 TGGCCA EarI 79 CTCTTC CTCTTC(1/4) 88 CTCTTC EcoRII 807 CCAGG ^CC[AT]GG 937 CCAGG HphI 665 TCACC GGTGA(8/7) 803 TCACC Ksp632I 79 CTCTTC CTCTTC(1/4) 88 CTCTTC MaeI 413 CTAG C^TAG 791 CTAG MluNI 371 TGGCCA TGG^CCA 623 TGGCCA MscI 371 TGGCCA TGG^CCA 623 TGGCCA MvaI 807 CCAGG CC^[AT]GG 937 CCAGG NcoI 350 CCATGG C^CATGG 474 CCATGG ScrFI 807 CCAGG CC^[ACGT]GG 937 CCAGG SfcI 378 CTGCAG C^T[GA][CT]AG 720 CTGTAG Tsp45I 25 GTGAC ^GT[GC]AC 536 GTGAC Tsp509I 99 AATT ^AATT 141 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI AlwI BbvI BsaJI BstF5I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII PvuII Sau3AI SfaNI StyI TfiI Tru9I TseI TspRI