################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tth111I Van91I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Aor51HI 56 AGCGCT AGC^GCT ApaI 213 GGGCCC GGGCC^C AvaI 209 CCCGGG C^[CT]CG[GA]G BbsI 602 GAAGAC GAAGAC(2/6) BbvI 632 GCAGC GCAGC(8/12) BciVI 268 GGATAC GTATCC(6/5) BpuAI 602 GAAGAC GAAGAC(2/6) BseRI 82 CTCCTC GAGGAG(10/8) BsgI 360 GTGCAG GTGCAG(16/14) BsiEI 225 CGATCG CG[GA][CT]^CG BsoBI 209 CCCGGG C^[CT]CG[GA]G DraI 375 TTTAAA TTT^AAA DraII 212 GGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 75 TGGCCG [CT]^GGCC[GA] Eco47III 56 AGCGCT AGC^GCT EcoO109I 212 GGGGCCC [GA]G^G[ACGT]CC[CT] HaeII 56 AGCGCT [GA]GCGC^[CT] MseI 376 TTAA T^TAA NlaIII 474 CATG CATG^ NruI 222 TCGCGA TCG^CGA PvuI 225 CGATCG CGAT^CG SacI 175 GAGCTC GAGCT^C SfaNI 418 GATGC GCATC(5/9) SfcI 237 CTACAG C^T[GA][CT]AG SmaI 209 CCCGGG CCC^GGG SspI 523 AATATT AAT^ATT Tru9I 376 TTAA T^TAA TseI 632 GCAGC G^C[AT]GC XmaCI 209 CCCGGG C^CCGGG XmaI 209 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 10 GTCTAC GT^[AC][GT]AC 91 GTAGAC AfaI 108 GTAC GT^AC 320 GTAC AspHI 175 GAGCTC G[AT]GC[AT]^C 202 GAGCAC BsaI 590 GGTCTC GGTCTC(1/5) 824 GGTCTC BsaJI 209 CCCGGG C^C[ACGT][ACGT]GG 210 CCGGGG BsiHKAI 175 GAGCTC G[AT]GC[AT]^C 202 GAGCAC BspMI 397 GCAGGT ACCTGC(4/8) 821 GCAGGT BsrI 495 ACTGG ACTGG(1/-1) 891 CCAGT FauI 134 CCCGC CCCGC(4/6) 216 CCCGC HapII 39 CCGG C^CGG 210 CCGG HinfI 130 GACTC G^A[ACGT]TC 621 GAGTC HpaII 39 CCGG C^CGG 210 CCGG HphI 400 GGTGA GGTGA(8/7) 859 GGTGA MaeII 35 ACGT A^CGT 702 ACGT MboII 538 GAAGA GAAGA(8/7) 602 GAAGA MspI 39 CCGG C^CGG 210 CCGG PleI 130 GACTC GAGTC(4/5) 621 GAGTC RsaI 108 GTAC GT^AC 320 GTAC Tsp45I 230 GTGAC ^GT[GC]AC 609 GTCAC Tsp509I 450 AATT ^AATT 904 AATT TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^ 255 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaII BanII BcnI BfaI BglII BmyI BsaHI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HgaI HhaI Hin1I HinP1I ItaI MaeI MaeIII MboI MflI MnlI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TaqI TthHB8I XbaI XhoII