Restriction map for
pFN21AE5006
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
CpoI DraI DraII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 448 GGTACC G^GTACC
AclI 952 AACGTT AA^CGTT
AflIII 362 ACGTGT A^C[GA][CT]GT
AhdI 684 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 10 CCTGAGG CC^T[ACGT]AGG
Asp700I 574 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 448 GGTACC G^GTACC
AspEI 684 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 164 CTCGAG C^[CT]CG[GA]G
AvaII 321 GGACC G^G[AT]CC
BanI 448 GGTACC G^G[CT][GA]CC
BanII 161 GGGCTC G[GA]GC[CT]^C
BbsI 103 GAAGAC GAAGAC(2/6)
BcgI 681 GCAGACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 369 TGATCA T^GATCA
BglII 913 AGATCT A^GATCT
BmyI 161 GGGCTC G[AGT]GC[ACT]^C
BpuAI 103 GAAGAC GAAGAC(2/6)
BseRI 88 GAGGAG GAGGAG(10/8)
BsmAI 415 GTCTC GTCTC(1/5)
BsmFI 589 GGGAC GGGAC(10/14)
BsmI 289 GCATTC GAATGC(1/-1)
BsoBI 164 CTCGAG C^[CT]CG[GA]G
Bsp1286I 161 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 347 TGTACA T^GTACA
BspDI 529 ATCGAT AT^CGAT
BspMI 942 GCAGGT ACCTGC(4/8)
BsrDI 211 GCAATG GCAATG(2/0)
BsrGI 347 TGTACA T^GTACA
BssKI 508 CCTGG ^CC[ACGT]GG
BstF5I 330 GGATG GGATG(2/0)
BstNI 508 CCTGG CC^[AT]GG
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
ClaI 529 ATCGAT AT^CGAT
DraIII 353 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 178 CCATGG C^C[GA][CT]GG
Eam1105I 684 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
EcoRII 508 CCTGG ^CC[AT]GG
EcoT14I 178 CCATGG C^C[AT][AT]GG
FbaI 369 TGATCA T^GATCA
FokI 330 GGATG GGATG(9/13)
HaeIII 750 GGCC GG^CC
HphI 216 GGTGA GGTGA(8/7)
KpnI 448 GGTACC GGTAC^C
MslI 430 CATGCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 226 CAGCTG C[AC]G^C[GT]G
MvaI 508 CCTGG CC^[AT]GG
NcoI 178 CCATGG C^CATGG
NdeI 338 CATATG CA^TATG
NspI 816 GCATGT [GA]CATG^[CT]
PaeR7I 164 CTCGAG C^TCGAG
PflMI 252 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 635 GACTC GAGTC(4/5)
Psp1406I 952 AACGTT AA^CGTT
PvuII 226 CAGCTG CAG^CTG
ScaI 395 AGTACT AGT^ACT
ScrFI 508 CCTGG CC^[ACGT]GG
SfaNI 865 GATGC GCATC(5/9)
SmlI 164 CTCGAG C^T[CT][GA]AG
SspBI 347 TGTACA T^GTACA
StyI 178 CCATGG C^C[AT][AT]GG
Van91I 252 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 164 CTCGAG C^TCGAG
XmnI 574 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 127 AAATTT [GA]^AATT[CT]
197 AAATTC
ApoI 127 AAATTT [GA]^AATT[CT]
197 AAATTC
AseI 673 ATTAAT AT^TAAT
897 ATTAAT
AsnI 673 ATTAAT AT^TAAT
897 ATTAAT
BsaJI 178 CCATGG C^C[ACGT][ACGT]GG
507 CCCTGG
BsiYI 252 CCAGATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
639 CCTAACAGTGG
BslI 252 CCAGATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
639 CCTAACAGTGG
BspHI 39 TCATGA T^CATGA
137 TCATGA
BstYI 6 AGATCC [GA]^GATC[CT]
913 AGATCT
Cfr13I 321 GGACC G^G[ACGT]CC
750 GGCCC
EarI 43 GAAGAG CTCTTC(1/4)
274 GAAGAG
Ksp632I 43 GAAGAG CTCTTC(1/4)
274 GAAGAG
MaeII 362 ACGT A^CGT
953 ACGT
MflI 6 AGATCC [GA]^GATC[CT]
913 AGATCT
NlaIV 448 GGTACC GG[ACGT]^[ACGT]CC
564 GGAGCC
PshBI 673 ATTAAT AT^TAAT
897 ATTAAT
PstI 30 CTGCAG CTGCA^G
679 CTGCAG
RcaI 39 TCATGA T^CATGA
137 TCATGA
Sau96I 321 GGACC G^G[ACGT]CC
750 GGCCC
TaqI 165 TCGA T^CGA
530 TCGA
TfiI 141 GAATC G^A[AT]TC
426 GAATC
Tsp45I 217 GTGAC ^GT[GC]AC
359 GTGAC
TthHB8I 165 TCGA T^CGA
530 TCGA
XhoII 6 AGATCC [GA]^GATC[CT]
913 AGATCT
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsrI
DdeI DpnII Fnu4HI HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII RsaI Sau3AI SfcI
Tru9I TseI Tsp509I TspRI