(Circular) (Six-base) MAPSORT of: pFN21AE5006.seq Check: 8220 from: 1 to: 5668 pFN21AE5006 5668 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2453 2989 2453 Size: 536 5132 AclI AA'CG_TT Cuts at: 1563 2958 3795 4168 1563 Size: 1395 837 373 3063 Fragments arranged by size: 3063 1395 837 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2921 2927 3731 5115 5383 1632 Size: 1010 279 6 804 1384 268 1917 Fragments arranged by size: 1917 1384 1010 804 279 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2367 4567 5544 1203 Size: 1164 2200 977 1327 Fragments arranged by size: 2200 1327 1164 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 2694 4397 2694 Size: 1703 3965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2645 Size: 32 5636 AlwNI CAG_nnn'CTG Cuts at: 2015 3411 4983 2015 Size: 1396 1572 2700 Fragments arranged by size: 2700 1572 1396 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2132 2202 2977 3203 3355 5464 1493 Size: 639 70 775 226 152 2109 1697 Fragments arranged by size: 2109 1697 775 639 226 152 70 AseI AT'TA_AT Cuts at: 160 1032 2679 2903 4222 160 Size: 872 1647 224 1319 1606 Fragments arranged by size: 1647 1606 1319 872 224 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2993 1870 Size: 73 226 824 4545 Fragments arranged by size: 4545 824 226 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2437 2470 2437 Size: 33 5635 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 2989 4444 618 Size: 325 217 627 666 536 1455 1842 Fragments arranged by size: 1842 1455 666 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 2170 2987 1391 Size: 779 817 4072 Fragments arranged by size: 4072 817 779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 928 Size: 971 216 4481 Fragments arranged by size: 4481 971 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2675 2709 3859 3893 1903 Size: 34 738 34 1150 34 3678 Fragments arranged by size: 3678 1150 738 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 2374 1548 Size: 826 4842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2918 5663 2918 Size: 2745 2923 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 2368 1318 Size: 1050 4618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4328 1239 Size: 3089 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 2645 2675 1178 Size: 30 165 30 1002 30 210 30 4171 Fragments arranged by size: 4171 1002 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 1186 Size: 922 4746 BseYI C'CCAG_C Cuts at: 4871 5367 4871 Size: 496 5172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 2294 3179 3272 2294 Size: 885 93 4690 Fragments arranged by size: 4690 885 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2987 3729 3814 4885 1391 Size: 399 380 817 742 85 1071 2174 Fragments arranged by size: 2174 1071 817 742 399 380 85 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 2044 2142 3557 5287 2044 Size: 98 1415 1730 2425 Fragments arranged by size: 2425 1730 1415 98 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2938 3023 844 Size: 2094 85 3489 Fragments arranged by size: 3489 2094 85 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2223 4163 4337 65 Size: 1183 975 1940 174 1396 Fragments arranged by size: 1940 1396 1183 975 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 2352 96 Size: 2256 3412 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2011 2918 2998 3365 3750 3767 5219 5305 Size: 39 907 80 367 385 17 1452 86 Cuts at: 5305 5317 5663 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 907 385 367 346 86 80 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 2016 Size: 5668 BtgI C'CryG_G Cuts at: 513 1065 2004 2183 513 Size: 552 939 179 3998 Fragments arranged by size: 3998 939 552 179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2355 3184 3978 3998 2355 Size: 829 794 20 4025 Fragments arranged by size: 4025 829 794 20 ClaI AT'CG_AT Cuts at: 1554 2535 1554 Size: 981 4687 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DraIII CAC_nnn'GTG Cuts at: 2363 2363 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3115 4004 5633 Size: 54 1425 50 275 18 1285 889 1629 Cuts at: 5633 8 Size: 43 Fragments arranged by size: 1629 1425 1285 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2274 3598 1353 Size: 690 231 1324 3423 Fragments arranged by size: 3423 1324 690 231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2642 2921 2927 3731 4328 5115 5383 Size: 393 1010 279 6 804 597 787 268 Cuts at: 5383 1239 Size: 1524 Fragments arranged by size: 1524 1010 804 787 597 393 279 268 6 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 4815 1678 Size: 3137 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2490 2522 2680 2712 4309 Size: 32 135 32 918 32 158 32 1597 Cuts at: 4309 4341 4383 4415 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1597 918 158 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2457 2993 2457 Size: 536 5132 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3846 Fragments arranged by size: 3846 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 3626 3985 4144 518 Size: 648 1273 1187 359 159 2042 Fragments arranged by size: 2042 1273 1187 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 3084 3761 4909 5154 1606 Size: 627 851 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 677 627 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2183 513 Size: 552 939 179 3998 Fragments arranged by size: 3998 939 552 179 NdeI CA'TA_TG Cuts at: 387 2344 387 Size: 1957 3711 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2825 3026 4571 1132 Size: 97 1596 201 1545 2229 Fragments arranged by size: 2229 1596 1545 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 1108 Size: 1155 4513 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2645 2677 3737 3769 5250 5282 2645 Size: 32 1060 32 1481 32 3031 Fragments arranged by size: 3031 1481 1060 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspXI vC'TCGA_Gb Cuts at: 2169 2169 Size: 5668 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2837 2869 2837 Size: 32 5636 PstI C_TGCA'G Cuts at: 830 2039 2688 3020 830 Size: 1209 649 332 3478 Fragments arranged by size: 3478 1209 649 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2233 2233 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 2402 3916 5628 2402 Size: 1514 1712 2442 Fragments arranged by size: 2442 1712 1514 SfcI C'TryA_G Cuts at: 826 1045 2035 2289 2684 3016 4151 4832 5023 Size: 219 990 254 395 332 1135 681 191 Cuts at: 5023 826 Size: 1471 Fragments arranged by size: 1471 1135 990 681 395 332 254 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2169 3772 4673 4935 820 Size: 197 926 226 1603 901 262 1553 Fragments arranged by size: 1603 1553 926 901 262 226 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2004 2183 3107 513 Size: 552 939 179 924 3074 Fragments arranged by size: 3074 939 924 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2343 3695 3712 3865 4050 4495 325 Size: 1255 763 1352 17 153 185 445 1498 Fragments arranged by size: 1498 1352 1255 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 2400 3914 Size: 274 80 33 51 1443 375 48 1514 Cuts at: 3914 5626 96 Size: 1712 138 Fragments arranged by size: 1712 1514 1443 375 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 5628 5660 2616 Size: 32 2980 32 2624 Fragments arranged by size: 2980 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 2169 1943 Size: 226 5442 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2583 3797 2583 Size: 1214 4454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI