################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AccI AccII AccIII AclI AflII AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 997 GACGTC GACGT^C Acc65I 179 GGTACC G^GTACC AcyI 997 GACGTC G[GA]^CG[CT]C AgeI 209 ACCGGT A^CCGGT ApaI 1102 GGGCCC GGGCC^C Asp718I 179 GGTACC G^GTACC AvaI 366 CTCGAG C^[CT]CG[GA]G AvrII 1098 CCTAGG C^CTAGG BalI 935 TGGCCA TGG^CCA BanI 179 GGTACC G^G[CT][GA]CC BbrPI 1255 CACGTG CAC^GTG BglI 437 GCCGGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 136 AGATCT A^GATCT BlnI 1098 CCTAGG C^CTAGG BsaAI 1255 CACGTG [CT]AC^GT[GA] BsaHI 997 GACGTC G[GA]^CG[CT]C BsiEI 1191 CGGCCG CG[GA][CT]^CG BsmFI 111 GTCCC GGGAC(10/14) BsmI 1016 GCATTC GAATGC(1/-1) BsoBI 366 CTCGAG C^[CT]CG[GA]G BstAPI 1007 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 424 GGTCACC G^GT[ACGT]ACC BstPI 424 GGTCACC G^GT[ACGT]ACC BstXI 926 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 615 CCACGG C^C[GA][CT]GG EagI 1191 CGGCCG C^GGCCG EclXI 1191 CGGCCG C^GGCCG Eco52I 1191 CGGCCG C^GGCCG EcoNI 167 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 424 GGTCACC G^GT[ACGT]ACC EcoRI 807 GAATTC G^AATTC EcoT14I 1098 CCTAGG C^C[AT][AT]GG EcoT22I 1 ATGCAT ATGCA^T Hin1I 997 GACGTC G[GA]^CG[CT]C KpnI 179 GGTACC GGTAC^C MluNI 935 TGGCCA TGG^CCA MscI 935 TGGCCA TGG^CCA NsiI 1 ATGCAT ATGCA^T PaeR7I 366 CTCGAG C^TCGAG PflMI 229 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 209 ACCGGT A^CCGGT PmaCI 1255 CACGTG CAC^GTG PmlI 1255 CACGTG CAC^GTG PpuMI 70 AGGTCCT [GA]G^G[AT]CC[CT] SacI 640 GAGCTC GAGCT^C SapI 217 GAAGAGC GCTCTTC(1/4) ScaI 1028 AGTACT AGT^ACT SfcI 75 CTACAG C^T[GA][CT]AG SphI 315 GCATGC GCATG^C SspI 1145 AATATT AAT^ATT StyI 1098 CCTAGG C^C[AT][AT]GG TaqI 367 TCGA T^CGA TfiI 759 GAATC G^A[AT]TC TthHB8I 367 TCGA T^CGA Van91I 229 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 872 CCAGCATCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 366 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 807 GAATTC [GA]^AATT[CT] 1291 GAATTT AflIII 623 ACATGT A^C[GA][CT]GT 705 ACATGT AocI 958 CCTGAGG CC^T[ACGT]AGG 1360 CCTGAGG ApoI 807 GAATTC [GA]^AATT[CT] 1291 GAATTT Asp700I 678 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1143 GAAATATTTC BpmI 1002 CTGGAG CTGGAG(16/14) 1249 CTGGAG BsaWI 209 ACCGGT [AT]^CCGG[AT] 302 ACCGGA BsgI 1333 CTGCAC GTGCAG(16/14) 1258 GTGCAG BsmAI 96 GAGAC GTCTC(1/5) 471 GTCTC BspLU11I 623 ACATGT A^CATGT 705 ACATGT BsrFI 209 ACCGGT [GA]^CCGG[CT] 1193 GCCGGT BsrI 602 ACTGG ACTGG(1/-1) 1317 ACTGG BstYI 136 AGATCT [GA]^GATC[CT] 1094 AGATCC Bsu36I 958 CCTGAGG CC^T[ACGT]AGG 1360 CCTGAGG Cfr10I 209 ACCGGT [GA]^CCGG[CT] 1193 GCCGGT DraIII 427 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1075 CACCTGGTG Eco81I 958 CCTGAGG CC^T[ACGT]AGG 1360 CCTGAGG FauI 308 CCCGC CCCGC(4/6) 954 CCCGC HaeII 188 AGCGCC [GA]GCGC^[CT] 358 AGCGCC MflI 136 AGATCT [GA]^GATC[CT] 1094 AGATCC MspA1I 763 CAGCTG C[AC]G^C[GT]G 1261 CAGCTG PleI 1243 GACTC GAGTC(4/5) 469 GAGTC PvuII 763 CAGCTG CAG^CTG 1261 CAGCTG SmlI 366 CTCGAG C^T[CT][GA]AG 970 CTTGAG Tsp509I 808 AATT ^AATT 1292 AATT XhoII 136 AGATCT [GA]^GATC[CT] 1094 AGATCC XmnI 678 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1143 GAAATATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrBI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI TseI Tsp45I TspRI