(Circular) (Six-base) MAPSORT of: pFN21AE5005.seq Check: 5923 from: 1 to: 6106 pFN21AE5005 6106 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3006 3916 278 Size: 53 83 186 936 1470 910 2468 Fragments arranged by size: 2468 1470 936 910 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 2184 3427 2184 Size: 1243 4863 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 2696 3248 4169 5553 5821 1632 Size: 878 186 552 921 1384 268 1917 Fragments arranged by size: 1917 1384 921 878 552 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 2628 2710 5005 5982 1203 Size: 1425 82 2295 977 1327 Fragments arranged by size: 2295 1425 1327 977 82 AgeI A'CCGG_T Cuts at: 2214 4937 2214 Size: 2723 3383 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 4336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 3849 5421 3849 Size: 1572 4534 ApaI G_GGCC'C Cuts at: 3111 3111 Size: 6106 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2812 3296 3415 3641 3793 5902 1493 Size: 1319 484 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1319 484 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2371 3431 1870 Size: 73 428 1060 4545 Fragments arranged by size: 4545 1060 428 73 AvrII C'CTAG_G Cuts at: 3103 3103 Size: 6106 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 3427 4882 618 Size: 325 217 627 397 1243 1455 1842 Fragments arranged by size: 1842 1455 1243 627 397 325 217 BanII G_rGCy'C Cuts at: 1391 2234 2649 3111 3425 1391 Size: 843 415 462 314 4072 Fragments arranged by size: 4072 843 462 415 314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4297 4331 1903 Size: 34 50 34 2276 34 3678 Fragments arranged by size: 3678 2276 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 4717 136 Size: 107 122 71 631 605 776 2269 1525 Fragments arranged by size: 2269 1525 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 2141 6101 2141 Size: 3960 2146 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2992 3111 4167 5323 1790 Size: 1202 119 1056 1156 2573 Fragments arranged by size: 2573 1202 1156 1056 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3837 4795 442 Size: 416 53 83 399 24 2420 958 1753 Fragments arranged by size: 2420 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3205 3205 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3028 3275 4766 1239 Size: 1789 247 1491 2579 Fragments arranged by size: 2579 1789 1491 247 Bpu10I CC'TnA_GC Cuts at: 2051 2051 Size: 6106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 4231 5096 5394 2996 Size: 1235 865 298 3708 Fragments arranged by size: 3708 1235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3262 3897 493 Size: 711 2058 635 2702 Fragments arranged by size: 2702 2058 711 635 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3003 3913 4295 4930 Size: 53 83 186 936 1470 910 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1470 1451 936 910 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 2307 4539 4937 5211 5358 5769 2214 Size: 93 2232 398 274 147 411 2551 Fragments arranged by size: 2551 2232 411 398 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5881 Fragments arranged by size: 5881 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2086 2194 2359 2870 3298 3383 1186 Size: 897 3 108 165 511 428 85 3909 Fragments arranged by size: 3909 897 511 428 165 108 85 3 BseYI C'CCAG_C Cuts at: 2980 2985 3309 5309 5805 2980 Size: 5 324 2000 496 3281 Fragments arranged by size: 3281 2000 496 324 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 3323 1163 Size: 573 1548 39 3946 Fragments arranged by size: 3946 1548 573 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3556 4317 5345 1540 Size: 275 186 1198 357 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 357 275 186 BsiHKAI G_wGCw'C Cuts at: 2550 2649 2844 2925 3081 3261 4167 4252 5323 Size: 99 195 81 156 180 906 85 1071 Cuts at: 5323 2550 Size: 3333 Fragments arranged by size: 3333 1071 906 195 180 156 99 85 81 BsmI GAATG_Cn' Cuts at: 3021 3617 3710 3021 Size: 596 93 5417 Fragments arranged by size: 5417 596 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2550 2649 2844 2925 2992 3081 Size: 399 444 316 99 195 81 67 89 Cuts at: 3081 3111 3261 3425 4167 4252 5323 1391 Size: 30 150 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 316 195 164 150 99 89 85 81 67 30 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 844 Size: 2617 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2227 3373 3993 1394 Size: 429 205 199 1146 620 3507 Fragments arranged by size: 3507 1146 620 429 205 199 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2214 3198 4750 4937 1700 Size: 110 404 984 1552 187 2869 Fragments arranged by size: 2869 1552 984 404 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 3018 794 Size: 2224 3882 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 2429 1184 Size: 1245 4861 BstXI CCAn_nnnn'nTGG Cuts at: 2938 2938 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2141 3099 3436 3803 4188 4205 5657 5743 Size: 169 958 337 367 385 17 1452 86 Cuts at: 5743 5755 6101 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 958 385 367 346 337 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2964 3366 2964 Size: 402 5704 BtgI C'CryG_G Cuts at: 513 1065 2620 513 Size: 552 1555 3999 Fragments arranged by size: 3999 1555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2620 487 Size: 683 56 1394 3973 Fragments arranged by size: 3973 1394 683 56 BtsI GCAGTG_nn' Cuts at: 2329 2908 3622 4416 4436 2329 Size: 579 714 794 20 3999 Fragments arranged by size: 3999 794 714 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DraIII CAC_nnn'GTG Cuts at: 2437 3085 2437 Size: 648 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2940 3196 3324 Size: 54 1425 50 275 18 1110 256 128 Cuts at: 3324 3553 4442 6071 8 Size: 229 889 1629 43 Fragments arranged by size: 1629 1425 1110 889 275 256 229 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 3553 1537 Size: 275 1384 357 4090 Fragments arranged by size: 4090 1384 357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2217 3059 3224 4036 1353 Size: 707 157 842 165 812 3423 Fragments arranged by size: 3423 842 812 707 165 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 2647 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 2696 3028 3248 3275 4169 4766 Size: 393 878 186 332 220 27 894 597 Cuts at: 4766 5553 5821 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 878 787 597 393 332 268 220 186 27 EcoNI CCTnn'n_nnAGG Cuts at: 2176 2176 Size: 6106 EcoO109I rG'GnC_Cy Cuts at: 2076 3107 3166 3331 2076 Size: 1031 59 165 4851 Fragments arranged by size: 4851 1031 165 59 EcoRI G'AATT_C Cuts at: 2812 3415 3793 2812 Size: 603 378 5125 Fragments arranged by size: 5125 603 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 6106 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2197 2367 5253 1678 Size: 519 170 2886 2531 Fragments arranged by size: 2886 2531 519 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2846 2878 3359 3391 4747 Size: 32 135 32 1274 32 481 32 1356 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 1274 481 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 2188 3431 2188 Size: 1243 4863 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2942 10 Size: 54 1425 343 1110 3174 Fragments arranged by size: 3174 1425 1110 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2215 2885 2936 4064 4423 4582 518 Size: 648 1049 670 51 1128 359 159 2042 Fragments arranged by size: 2042 1128 1049 670 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2770 3268 3522 4199 5347 5592 1606 Size: 1164 498 254 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 677 498 254 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5554 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2324 2632 2714 3241 3464 5009 1132 Size: 97 1095 308 82 527 223 1545 2229 Fragments arranged by size: 2229 1545 1095 527 308 223 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 2628 2710 5005 2628 Size: 82 2295 3729 Fragments arranged by size: 3729 2295 82 PflMI CCAn_nnn'nTGG Cuts at: 1108 2240 1108 Size: 1132 4974 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PmlI CAC'GTG Cuts at: 3262 3262 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2076 2076 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 3107 3107 Size: 6106 PstI C_TGCA'G Cuts at: 830 3458 830 Size: 2628 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2770 3268 2770 Size: 498 5608 SacI G_AGCT'C Cuts at: 2649 2649 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 2217 2217 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 3035 4354 6066 3035 Size: 1319 1712 3075 Fragments arranged by size: 3075 1712 1319 SexAI A'CCwGG_T Cuts at: 2348 2433 3081 2348 Size: 85 648 5373 Fragments arranged by size: 5373 648 85 SfcI C'TryA_G Cuts at: 826 1045 2080 3454 4589 5270 5461 826 Size: 219 1035 1374 1135 681 191 1471 Fragments arranged by size: 1471 1374 1135 1035 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2371 2975 4210 5111 5373 820 Size: 197 926 428 604 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 604 428 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2324 3464 1132 Size: 1192 1140 3774 Fragments arranged by size: 3774 1192 1140 SspI AAT'ATT Cuts at: 5 52 3152 4030 5 Size: 47 3100 878 2081 Fragments arranged by size: 3100 2081 878 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 3103 3545 513 Size: 552 2038 442 3074 Fragments arranged by size: 3074 2038 552 442 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2136 4133 4150 4303 4488 4933 325 Size: 1255 556 1997 17 153 185 445 1498 Fragments arranged by size: 1997 1498 1255 556 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 4352 6064 Size: 274 80 33 51 1443 1056 1319 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1712 1443 1319 1056 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3111 3143 6066 6098 3111 Size: 32 2923 32 3119 Fragments arranged by size: 3119 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2884 2884 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 2371 1943 Size: 428 5678 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 2687 3152 4235 2687 Size: 465 1083 4558 Fragments arranged by size: 4558 1083 465 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3004 3914 276 Size: 53 83 186 936 1470 910 2468 Fragments arranged by size: 2468 1470 936 910 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI