(Linear) (Six-base) MAPSORT of: KIEE5005.seq Check: 1919 from: 1 to: 1401 KIEE5005.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AatII G_ACGT'C Cuts at: 0 1001 1401 Size: 1001 400 Acc65I G'GTAC_C Cuts at: 0 179 1401 Size: 179 1222 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 691 1243 1401 Size: 505 186 552 158 Fragments arranged by size: 552 505 186 158 AflIII A'CryG_T Cuts at: 0 623 705 1401 Size: 623 82 696 Fragments arranged by size: 696 623 82 AgeI A'CCGG_T Cuts at: 0 209 1401 Size: 209 1192 AleI CACnn'nnGTG Cuts at: 0 931 1401 Size: 931 470 ApaI G_GGCC'C Cuts at: 0 1106 1401 Size: 1106 295 ApoI r'AATT_y Cuts at: 0 807 1291 1401 Size: 807 484 110 Fragments arranged by size: 807 484 110 AvaI C'yCGr_G Cuts at: 0 366 1401 Size: 366 1035 AvrII C'CTAG_G Cuts at: 0 1098 1401 Size: 1098 303 BanI G'GyrC_C Cuts at: 0 179 1401 Size: 179 1222 BanII G_rGCy'C Cuts at: 0 229 644 1106 1401 Size: 229 415 462 295 Fragments arranged by size: 462 415 295 229 BfrBI ATG'CAT Cuts at: 0 3 1401 Size: 3 1398 BglI GCCn_nnn'nGGC Cuts at: 0 443 1401 Size: 443 958 BglII A'GATC_T Cuts at: 0 136 1401 Size: 136 1265 Bme1580I G_kGCm'C Cuts at: 0 987 1106 1401 Size: 987 119 295 Fragments arranged by size: 987 295 119 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1200 1401 Size: 1200 201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1023 1270 1401 Size: 1023 247 131 Fragments arranged by size: 1023 247 131 Bpu10I CC'TnA_GC Cuts at: 0 46 1401 Size: 46 1355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 991 1401 Size: 991 410 BsaAI yAC'GTr Cuts at: 0 1257 1401 Size: 1257 144 BsaHI Gr'CG_yC Cuts at: 0 998 1401 Size: 998 403 BsaWI w'CCGG_w Cuts at: 0 209 302 1401 Size: 209 93 1099 Fragments arranged by size: 1099 209 93 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 78 81 189 354 865 1293 1378 1401 Size: 78 3 108 165 511 428 85 23 Fragments arranged by size: 511 428 165 108 85 78 23 3 BseYI C'CCAG_C Cuts at: 0 975 980 1304 1401 Size: 975 5 324 97 Fragments arranged by size: 975 324 97 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1279 1318 1401 Size: 1279 39 83 Fragments arranged by size: 1279 83 39 BsiEI CG_ry'CG Cuts at: 0 1194 1401 Size: 1194 207 BsiHKAI G_wGCw'C Cuts at: 0 545 644 839 920 1076 1256 1401 Size: 545 99 195 81 156 180 145 Fragments arranged by size: 545 195 180 156 145 99 81 BsmI GAATG_Cn' Cuts at: 0 1016 1401 Size: 1016 385 Bsp1286I G_dGCh'C Cuts at: 0 229 545 644 839 920 987 1076 1106 Size: 229 316 99 195 81 67 89 30 Cuts at: 1106 1256 1401 Size: 150 145 Fragments arranged by size: 316 229 195 150 145 99 89 81 67 30 BsrBI CCG'CTC Cuts at: 0 23 222 1368 1401 Size: 23 199 1146 33 Fragments arranged by size: 1146 199 33 23 BsrFI r'CCGG_y Cuts at: 0 209 1193 1401 Size: 209 984 208 Fragments arranged by size: 984 209 208 BstAPI GCAn_nnn'nTGC Cuts at: 0 1013 1401 Size: 1013 388 BstEII G'GTnAC_C Cuts at: 0 424 1401 Size: 424 977 BstXI CCAn_nnnn'nTGG Cuts at: 0 933 1401 Size: 933 468 BstYI r'GATC_y Cuts at: 0 136 1094 1401 Size: 136 958 307 Fragments arranged by size: 958 307 136 Bsu36I CC'TnA_GG Cuts at: 0 959 1361 1401 Size: 959 402 40 Fragments arranged by size: 959 402 40 BtgI C'CryG_G Cuts at: 0 615 1401 Size: 615 786 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 615 1401 Size: 615 786 BtsI GCAGTG_nn' Cuts at: 0 324 903 1401 Size: 324 579 498 Fragments arranged by size: 579 498 324 DraIII CAC_nnn'GTG Cuts at: 0 432 1080 1401 Size: 432 648 321 Fragments arranged by size: 648 432 321 EaeI y'GGCC_r Cuts at: 0 935 1191 1319 1401 Size: 935 256 128 82 Fragments arranged by size: 935 256 128 82 EagI C'GGCC_G Cuts at: 0 1191 1401 Size: 1191 210 EarI CTCTTCn'nnn_ Cuts at: 0 55 212 1054 1219 1401 Size: 55 157 842 165 182 Fragments arranged by size: 842 182 165 157 55 EcoICRI GAG'CTC Cuts at: 0 642 1401 Size: 642 759 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 691 1023 1243 1270 1401 Size: 505 186 332 220 27 131 Fragments arranged by size: 505 332 220 186 131 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 171 1401 Size: 171 1230 EcoO109I rG'GnC_Cy Cuts at: 0 71 1102 1161 1326 1401 Size: 71 1031 59 165 75 Fragments arranged by size: 1031 165 75 71 59 EcoRI G'AATT_C Cuts at: 0 807 1401 Size: 807 594 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 675 1401 Size: 675 726 HaeII r_GCGC'y Cuts at: 0 192 362 1401 Size: 192 170 1039 Fragments arranged by size: 1039 192 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 841 873 1354 1386 1401 Size: 841 32 481 32 15 Fragments arranged by size: 841 481 32 32 15 KpnI G_GTAC'C Cuts at: 0 183 1401 Size: 183 1218 MscI TGG'CCA Cuts at: 0 937 1401 Size: 937 464 MslI CAynn'nnrTG Cuts at: 0 210 880 931 1401 Size: 210 670 51 470 Fragments arranged by size: 670 470 210 51 MspA1I CmG'CkG Cuts at: 0 765 1263 1401 Size: 765 498 138 Fragments arranged by size: 765 498 138 NsiI A_TGCA'T Cuts at: 0 5 1401 Size: 5 1396 NspI r_CATG'y Cuts at: 0 319 627 709 1236 1401 Size: 319 308 82 527 165 Fragments arranged by size: 527 319 308 165 82 PciI A'CATG_T Cuts at: 0 623 705 1401 Size: 623 82 696 Fragments arranged by size: 696 623 82 PflMI CCAn_nnn'nTGG Cuts at: 0 235 1401 Size: 235 1166 PmlI CAC'GTG Cuts at: 0 1257 1401 Size: 1257 144 PpuMI rG'GwC_Cy Cuts at: 0 71 1401 Size: 71 1330 PspOMI G'GGCC_C Cuts at: 0 1102 1401 Size: 1102 299 PvuII CAG'CTG Cuts at: 0 765 1263 1401 Size: 765 498 138 Fragments arranged by size: 765 498 138 SacI G_AGCT'C Cuts at: 0 644 1401 Size: 644 757 SapI GCTCTTCn'nnn_ Cuts at: 0 212 1401 Size: 212 1189 ScaI AGT'ACT Cuts at: 0 1030 1401 Size: 1030 371 SexAI A'CCwGG_T Cuts at: 0 343 428 1076 1401 Size: 343 85 648 325 Fragments arranged by size: 648 343 325 85 SfcI C'TryA_G Cuts at: 0 75 1401 Size: 75 1326 SmlI C'TyrA_G Cuts at: 0 366 970 1401 Size: 366 604 431 Fragments arranged by size: 604 431 366 SphI G_CATG'C Cuts at: 0 319 1401 Size: 319 1082 SspI AAT'ATT Cuts at: 0 1147 1401 Size: 1147 254 StyI C'CwwG_G Cuts at: 0 1098 1401 Size: 1098 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 131 1401 Size: 131 1270 TatI w'GTAC_w Cuts at: 0 1028 1401 Size: 1028 373 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1106 1138 1401 Size: 1106 32 263 Fragments arranged by size: 1106 263 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 879 1401 Size: 879 522 XhoI C'TCGA_G Cuts at: 0 366 1401 Size: 366 1035 XmnI GAAnn'nnTTC Cuts at: 0 682 1147 1401 Size: 682 465 254 Fragments arranged by size: 682 465 254 ZraI GAC'GTC Cuts at: 0 999 1401 Size: 999 402 Enzymes that do cut: AatII Acc65I AcuI AflIII AgeI AleI ApaI ApoI AvaI AvrII BanI BanII BfrBI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI MscI MslI MspA1I NsiI NspI PciI PflMI PmlI PpuMI PspOMI PvuII SacI SapI ScaI SexAI SfcI SmlI SphI SspI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BlpI BmgBI BmrI BmtI BsaI BsaBI BsaXI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DrdI EciI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XmaI