################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspMI BsrBI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 40 GTAGAC GT^[AC][GT]AC AciI 145 CCGC CCGC(-3/-1) AflII 766 CTTAAG C^TTAAG AflIII 937 ACATGT A^C[GA][CT]GT Aor51HI 266 AGCGCT AGC^GCT AspHI 1085 GAGCAC G[AT]GC[AT]^C AvaI 578 CTCGGG C^[CT]CG[GA]G BalI 442 TGGCCA TGG^CCA BbsI 571 GAAGAC GAAGAC(2/6) BbvCI 5 CCTCAGC CCTCAGC(-5/-2) BfrI 766 CTTAAG C^TTAAG BmyI 1085 GAGCAC G[AGT]GC[ACT]^C BpuAI 571 GAAGAC GAAGAC(2/6) BsaI 110 GGTCTC GGTCTC(1/5) BseRI 20 CTCCTC GAGGAG(10/8) BsiHKAI 1085 GAGCAC G[AT]GC[AT]^C BsiYI 1065 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1065 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 111 GTCTC GTCTC(1/5) BsmFI 587 GGGAC GGGAC(10/14) BsoBI 578 CTCGGG C^[CT]CG[GA]G Bsp1286I 1085 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 1220 TGTACA T^GTACA BspHI 1115 TCATGA T^CATGA BspLU11I 937 ACATGT A^CATGT BsrGI 1220 TGTACA T^GTACA CfoI 267 GCGC GCG^C EaeI 442 TGGCCA [CT]^GGCC[GA] Eco47III 266 AGCGCT AGC^GCT EcoRI 496 GAATTC G^AATTC EcoT14I 963 CCTTGG C^C[AT][AT]GG HaeII 266 AGCGCT [GA]GCGC^[CT] HapII 1201 CCGG C^CGG HhaI 267 GCGC GCG^C HinP1I 267 GCGC G^CGC HincII 132 GTTAAC GT[CT]^[GA]AC HindII 132 GTTAAC GT[CT]^[GA]AC HinfI 1197 GAATC G^A[ACGT]TC HpaI 132 GTTAAC GTT^AAC HpaII 1201 CCGG C^CGG HphI 792 GGTGA GGTGA(8/7) MaeIII 389 GTGAC ^GT[ACGT]AC MluNI 442 TGGCCA TGG^CCA MscI 442 TGGCCA TGG^CCA MslI 700 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1201 CCGG C^CGG NspI 937 ACATGT [GA]CATG^[CT] PvuII 847 CAGCTG CAG^CTG RcaI 1115 TCATGA T^CATGA SfaNI 436 GCATC GCATC(5/9) SspBI 1220 TGTACA T^GTACA StyI 963 CCTTGG C^C[AT][AT]GG TfiI 1197 GAATC G^A[AT]TC Tsp45I 389 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 865 GTAC GT^AC 1221 GTAC BbvI 174 GCAGC GCAGC(8/12) 147 GCTGC BfaI 386 CTAG C^TAG 1058 CTAG BsaJI 577 CCTCGG C^C[ACGT][ACGT]GG 963 CCTTGG BsrDI 510 CATTGC GCAATG(2/0) 395 GCAATG BstYI 882 AGATCC [GA]^GATC[CT] 1353 GGATCT Cac8I 119 GCAAGC GC[ACGT]^[ACGT]GC 849 GCTGGC EarI 835 CTCTTC CTCTTC(1/4) 81 GAAGAG HaeIII 443 GGCC GG^CC 929 GGCC Ksp632I 835 CTCTTC CTCTTC(1/4) 81 GAAGAG MaeI 386 CTAG C^TAG 1058 CTAG MflI 882 AGATCC [GA]^GATC[CT] 1353 GGATCT MspA1I 145 CCGCTG C[AC]G^C[GT]G 847 CAGCTG RsaI 865 GTAC GT^AC 1221 GTAC TseI 147 GCTGC G^C[AT]GC 174 GCAGC XhoII 882 AGATCC [GA]^GATC[CT] 1353 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AluI AlwI AlwNI ApoI BpmI BsrI BstF5I DdeI DpnII Fnu4HI FokI ItaI MaeII MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI SmlI SspI Tru9I Tsp509I TspRI