(Linear) (Six-base) MAPSORT of: KIEE5003.seq Check: 513 from: 1 to: 1401 KIEE5003.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AccI GT'mk_AC Cuts at: 0 41 1401 Size: 41 1360 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 359 1401 Size: 184 175 1042 Fragments arranged by size: 1042 184 175 AfeI AGC'GCT Cuts at: 0 268 1401 Size: 268 1133 AflII C'TTAA_G Cuts at: 0 766 1401 Size: 766 635 AflIII A'CryG_T Cuts at: 0 937 1401 Size: 937 464 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 366 398 1401 Size: 366 32 1003 Fragments arranged by size: 1003 366 32 AlwNI CAG_nnn'CTG Cuts at: 0 314 552 789 873 1401 Size: 314 238 237 84 528 Fragments arranged by size: 528 314 238 237 84 ApoI r'AATT_y Cuts at: 0 400 466 496 535 614 922 1401 Size: 400 66 30 39 79 308 479 Fragments arranged by size: 479 400 308 79 66 39 30 AvaI C'yCGr_G Cuts at: 0 578 1401 Size: 578 823 BbsI GAAGACnn'nnnn_ Cuts at: 0 578 1401 Size: 578 823 BbvCI CC'TCA_GC Cuts at: 0 6 1401 Size: 6 1395 BmgBI CAC'GTC Cuts at: 0 1381 1401 Size: 1381 20 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 895 1378 1401 Size: 574 321 483 23 Fragments arranged by size: 574 483 321 23 Bpu10I CC'TnA_GC Cuts at: 0 6 1401 Size: 6 1395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 98 451 1401 Size: 98 353 950 Fragments arranged by size: 950 353 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 116 1401 Size: 116 1285 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 6 36 253 283 366 396 1401 Size: 6 30 217 30 83 30 1005 Fragments arranged by size: 1005 217 83 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 11 1401 Size: 11 1390 BseYI C'CCAG_C Cuts at: 0 505 1401 Size: 505 896 BsiHKAI G_wGCw'C Cuts at: 0 1089 1401 Size: 1089 312 Bsp1286I G_dGCh'C Cuts at: 0 1089 1401 Size: 1089 312 BspHI T'CATG_A Cuts at: 0 1115 1401 Size: 1115 286 BsrDI GCAATG_nn' Cuts at: 0 402 509 1401 Size: 402 107 892 Fragments arranged by size: 892 402 107 BsrGI T'GTAC_A Cuts at: 0 1220 1401 Size: 1220 181 BstYI r'GATC_y Cuts at: 0 882 1353 1401 Size: 882 471 48 Fragments arranged by size: 882 471 48 BtsI GCAGTG_nn' Cuts at: 0 211 1401 Size: 211 1190 EaeI y'GGCC_r Cuts at: 0 442 1401 Size: 442 959 EarI CTCTTCn'nnn_ Cuts at: 0 76 841 1401 Size: 76 765 560 Fragments arranged by size: 765 560 76 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 359 574 895 1378 1401 Size: 184 175 215 321 483 23 Fragments arranged by size: 483 321 215 184 175 23 EcoRI G'AATT_C Cuts at: 0 496 1401 Size: 496 905 HaeII r_GCGC'y Cuts at: 0 270 1401 Size: 270 1131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 148 180 223 255 632 664 1401 Size: 148 32 43 32 377 32 737 Fragments arranged by size: 737 377 148 43 32 32 32 HincII GTy'rAC Cuts at: 0 134 1401 Size: 134 1267 HpaI GTT'AAC Cuts at: 0 134 1401 Size: 134 1267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 525 1401 Size: 525 876 MscI TGG'CCA Cuts at: 0 444 1401 Size: 444 957 MslI CAynn'nnrTG Cuts at: 0 704 1401 Size: 704 697 MspA1I CmG'CkG Cuts at: 0 147 849 1401 Size: 147 702 552 Fragments arranged by size: 702 552 147 NspI r_CATG'y Cuts at: 0 941 1401 Size: 941 460 PciI A'CATG_T Cuts at: 0 937 1401 Size: 937 464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 366 398 943 975 1401 Size: 366 32 545 32 426 Fragments arranged by size: 545 426 366 32 32 PvuII CAG'CTG Cuts at: 0 849 1401 Size: 849 552 SmlI C'TyrA_G Cuts at: 0 113 430 766 1401 Size: 113 317 336 635 Fragments arranged by size: 635 336 317 113 SspI AAT'ATT Cuts at: 0 669 731 983 1401 Size: 669 62 252 418 Fragments arranged by size: 669 418 252 62 StyI C'CwwG_G Cuts at: 0 963 1401 Size: 963 438 TatI w'GTAC_w Cuts at: 0 864 1220 1401 Size: 864 356 181 Fragments arranged by size: 864 356 181 Enzymes that do cut: AccI AcuI AfeI AflII AflIII AloI AlwNI ApoI AvaI BbsI BbvCI BmgBI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrGI BstYI BtsI EaeI EarI Eco57MI EcoRI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NspI PciI PpiI PvuII SmlI SspI StyI TatI Enzymes that do not cut: AarI AatII Acc65I AclI AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HindIII KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI