(Circular) (Six-base) MAPSORT of: pFN21AE2538.seq Check: 9722 from: 1 to: 12397 pFN21AE2538 12397 bp With 182 enzymes: SgfI * February 10, 2017 15:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 8167 2599 Size: 5568 6829 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10207 278 Size: 53 83 186 936 8671 2468 Fragments arranged by size: 8671 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 2553 5099 5689 5938 9740 1937 Size: 397 219 2546 590 249 3802 4594 Fragments arranged by size: 4594 3802 2546 590 397 249 219 Acc65I G'GTAC_C Cuts at: 9718 9718 Size: 12397 AclI AA'CG_TT Cuts at: 1563 6992 10524 10897 1563 Size: 5429 3532 373 3063 Fragments arranged by size: 5429 3532 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2218 2312 2967 3552 4431 4446 4637 Size: 462 124 94 655 585 879 15 191 Cuts at: 4637 6068 6251 6349 6851 6960 7325 7376 8128 Size: 1431 183 98 502 109 365 51 752 Cuts at: 8128 8972 9531 9587 10460 11844 12112 1632 Size: 844 559 56 873 1384 268 1917 Fragments arranged by size: 1917 1431 1384 879 873 844 752 655 585 559 502 462 365 268 191 183 124 109 98 94 56 51 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12200 AflIII A'CryG_T Cuts at: 1203 2800 4969 5737 5891 8686 8906 11296 12273 Size: 1597 2169 768 154 2795 220 2390 977 Cuts at: 12273 1203 Size: 1327 Fragments arranged by size: 2795 2390 2169 1597 1327 977 768 220 154 AgeI A'CCGG_T Cuts at: 11228 11228 Size: 12397 AhdI GACnn_n'nnGTC Cuts at: 6097 11126 6097 Size: 5029 7368 AleI CACnn'nnGTG Cuts at: 1166 6894 1166 Size: 5728 6669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4078 1184 Size: 2894 9503 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3603 3635 3603 Size: 32 12365 AlwNI CAG_nnn'CTG Cuts at: 2972 4046 4592 7607 7871 8434 8699 9460 10140 Size: 1074 546 3015 264 563 265 761 680 Cuts at: 10140 11712 2972 Size: 1572 3657 Fragments arranged by size: 3657 3015 1572 1074 761 680 563 546 265 264 ApaI G_GGCC'C Cuts at: 6345 6345 Size: 12397 ApaLI G'TGCA_C Cuts at: 10454 11610 10454 Size: 1156 11241 ApoI r'AATT_y Cuts at: 1493 2427 2877 3023 3707 3715 3920 4522 4541 Size: 934 450 146 684 8 205 602 19 Cuts at: 4541 4903 5020 5227 5351 5430 5557 6068 8076 Size: 362 117 207 124 79 127 511 2008 Cuts at: 8076 9706 9932 10084 12193 1493 Size: 1630 226 152 2109 1697 Fragments arranged by size: 2109 2008 1697 1630 934 684 602 511 450 362 226 207 205 152 146 127 124 117 79 19 8 AseI AT'TA_AT Cuts at: 160 1032 3813 7926 10951 160 Size: 872 2781 4113 3025 1606 Fragments arranged by size: 4113 3025 2781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12397 AvaI C'yCGr_G Cuts at: 1870 1943 6777 9198 9722 1870 Size: 73 4834 2421 524 4545 Fragments arranged by size: 4834 4545 2421 524 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4335 4368 5843 5876 4335 Size: 33 1475 33 10856 Fragments arranged by size: 10856 1475 33 33 BamHI G'GATC_C Cuts at: 5923 6041 8536 9256 9727 10094 5923 Size: 118 2495 720 471 367 8226 Fragments arranged by size: 8226 2495 720 471 367 118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 8680 9718 11173 618 Size: 325 217 627 390 6503 1038 1455 1842 Fragments arranged by size: 6503 1842 1455 1038 627 390 325 217 BanII G_rGCy'C Cuts at: 1391 3068 3317 5769 6345 6535 7890 9716 1391 Size: 1677 249 2452 576 190 1355 1826 4072 Fragments arranged by size: 4072 2452 1826 1677 1355 576 249 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3410 4652 4936 5040 6566 8174 8978 Size: 971 1511 1242 284 104 1526 1608 804 Cuts at: 8978 9197 9519 928 Size: 219 322 3806 Fragments arranged by size: 3806 1608 1526 1511 1242 971 804 322 284 219 104 BbvCI CC'TCA_GC Cuts at: 9087 9087 Size: 12397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10588 10622 1903 Size: 34 8651 34 3678 Fragments arranged by size: 8651 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10289 11499 10289 Size: 1210 11187 BclI T'GATC_A Cuts at: 1548 3985 7732 8614 9234 1548 Size: 2437 3747 882 620 4711 Fragments arranged by size: 4711 3747 2437 882 620 BfrBI ATG'CAT Cuts at: 4146 5057 4146 Size: 911 11486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11008 136 Size: 107 122 71 631 605 9336 1525 Fragments arranged by size: 9336 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2711 3215 12392 2711 Size: 504 9177 2716 Fragments arranged by size: 9177 2716 504 BlpI GC'TnA_GC Cuts at: 3067 7211 7595 8622 9814 3067 Size: 4144 384 1027 1192 5650 Fragments arranged by size: 5650 4144 1192 1027 384 Bme1580I G_kGCm'C Cuts at: 1790 6321 6345 10458 11614 1790 Size: 4531 24 4113 1156 2573 Fragments arranged by size: 4531 4113 2573 1156 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3623 5702 5929 Size: 416 53 83 399 24 2206 2079 227 Cuts at: 5929 5961 8585 8713 8789 9436 10128 11086 442 Size: 32 2624 128 76 647 692 958 1753 Fragments arranged by size: 2624 2206 2079 1753 958 692 647 416 399 227 128 83 76 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12397 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3234 3256 6770 7207 3234 Size: 22 3514 437 8424 Fragments arranged by size: 8424 3514 437 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 3831 4704 5186 8743 9376 9614 11057 Size: 1059 1533 873 482 3557 633 238 1443 Cuts at: 11057 1239 Size: 2579 Fragments arranged by size: 3557 2579 1533 1443 1059 873 633 482 238 Bpu10I CC'TnA_GC Cuts at: 9087 9087 Size: 12397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3397 4875 5778 5958 6519 6715 8862 9317 10522 Size: 1478 903 180 561 196 2147 455 1205 Cuts at: 10522 11387 11685 3397 Size: 865 298 4109 Fragments arranged by size: 4109 2147 1478 1205 903 865 561 455 298 196 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3441 3491 3849 7193 7491 8187 11060 Size: 617 1942 50 358 3344 298 696 2873 Cuts at: 11060 882 Size: 2219 Fragments arranged by size: 3344 2873 2219 1942 696 617 358 298 50 BsaAI yAC'GTr Cuts at: 493 1204 2803 5814 5894 10188 493 Size: 711 1599 3011 80 4294 2702 Fragments arranged by size: 4294 3011 2702 1599 711 80 BsaBI GATnn'nnATC Cuts at: 3187 4614 7731 12391 3187 Size: 1427 3117 4660 3193 Fragments arranged by size: 4660 3193 3117 1427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 10204 10586 11221 275 Size: 53 83 186 936 8671 382 635 1451 Fragments arranged by size: 8671 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5425 9471 10830 11228 11502 11649 12060 5425 Size: 4046 1359 398 274 147 411 5762 Fragments arranged by size: 5762 4046 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4621 4651 4956 4986 7603 Size: 30 165 30 3218 30 305 30 2617 Cuts at: 7603 7633 7773 7803 7949 7979 8741 8771 9024 Size: 30 140 30 146 30 762 30 253 Cuts at: 9024 9054 9554 9584 1178 Size: 30 500 30 3991 Fragments arranged by size: 3991 3218 2617 762 500 305 253 165 146 140 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4971 6032 7089 7970 9141 1186 Size: 3785 1061 1057 881 1171 4442 Fragments arranged by size: 4442 3785 1171 1061 1057 881 BseYI C'CCAG_C Cuts at: 3839 6687 8540 8579 8976 11600 12096 3839 Size: 2848 1853 39 397 2624 496 4140 Fragments arranged by size: 4140 2848 2624 1853 496 397 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2259 8853 1163 Size: 573 393 130 6594 4707 Fragments arranged by size: 6594 4707 573 393 130 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7372 9847 10608 11636 1540 Size: 275 186 5371 2475 761 1028 2301 Fragments arranged by size: 5371 2475 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 3068 3317 3370 5769 6535 7212 7558 7601 Size: 1004 249 53 2399 766 677 346 43 Cuts at: 7601 8568 10458 10543 11614 2064 Size: 967 1890 85 1071 2847 Fragments arranged by size: 2847 2399 1890 1071 1004 967 766 677 346 249 85 53 43 BsmI GAATG_Cn' Cuts at: 6498 7814 9908 10001 6498 Size: 1316 2094 93 8894 Fragments arranged by size: 8894 2094 1316 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5204 5204 Size: 12397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 3068 3317 3370 5769 6321 6345 Size: 399 274 1004 249 53 2399 552 24 Cuts at: 6345 6535 7212 7558 7601 7890 8568 9716 10458 Size: 190 677 346 43 289 678 1148 742 Cuts at: 10458 10543 11614 1391 Size: 85 1071 2174 Fragments arranged by size: 2399 2174 1148 1071 1004 742 678 677 552 399 346 289 274 249 190 85 53 43 24 BspEI T'CCGG_A Cuts at: 5425 12060 5425 Size: 6635 5762 BspHI T'CATG_A Cuts at: 5107 6579 6882 10286 12016 5107 Size: 1472 303 3404 1730 5488 Fragments arranged by size: 5488 3404 1730 1472 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 4217 8167 9752 844 Size: 1755 1618 3950 1585 3489 Fragments arranged by size: 3950 3489 1755 1618 1585 BsrBI CCG'CTC Cuts at: 1394 1823 8272 10284 1394 Size: 429 6449 2012 3507 Fragments arranged by size: 6449 3507 2012 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 5917 9607 10892 11066 65 Size: 1183 1042 3627 3690 1285 174 1396 Fragments arranged by size: 3690 3627 1396 1285 1183 1042 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8782 11041 11228 1700 Size: 110 6972 2259 187 2869 Fragments arranged by size: 6972 2869 2259 187 110 BsrGI T'GTAC_A Cuts at: 96 3728 6506 8848 8962 96 Size: 3632 2778 2342 114 3531 Fragments arranged by size: 3632 3531 2778 2342 114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12397 BssSI C'ACGA_G Cuts at: 3369 10457 11469 3369 Size: 7088 1012 4297 Fragments arranged by size: 7088 4297 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 8425 794 Size: 7631 4766 BstBI TT'CG_AA Cuts at: 6719 11201 6719 Size: 4482 7915 BstEII G'GTnAC_C Cuts at: 1184 5549 7064 8501 1184 Size: 4365 1515 1437 5080 Fragments arranged by size: 5080 4365 1515 1437 BstXI CCAn_nnnn'nTGG Cuts at: 6381 6967 7016 8041 8738 6381 Size: 586 49 1025 697 10040 Fragments arranged by size: 10040 1025 697 586 49 BstYI r'GATC_y Cuts at: 1972 2711 3088 3215 4028 5923 6041 7344 7726 Size: 739 377 127 813 1895 118 1303 382 Cuts at: 7726 8536 9133 9202 9256 9727 10094 10479 10496 Size: 810 597 69 54 471 367 385 17 Cuts at: 10496 11948 12034 12046 12392 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1895 1452 1303 813 810 739 597 471 385 382 377 367 346 127 118 86 69 54 17 12 Bsu36I CC'TnA_GG Cuts at: 3452 9585 3452 Size: 6133 6264 BtgI C'CryG_G Cuts at: 513 1065 5028 5241 513 Size: 552 3963 213 7669 Fragments arranged by size: 7669 3963 552 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6394 7533 487 Size: 683 56 5168 1139 5351 Fragments arranged by size: 5351 5168 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2271 2468 2535 4740 6503 8450 9063 9913 10707 Size: 197 67 2205 1763 1947 613 850 794 Cuts at: 10707 10727 2271 Size: 20 3941 Fragments arranged by size: 3941 2205 1947 1763 850 794 613 197 67 20 ClaI AT'CG_AT Cuts at: 1554 3862 1554 Size: 2308 10089 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12362 DraI TTT'AAA Cuts at: 3021 3058 3078 3712 3885 4767 6772 9520 9701 Size: 37 20 634 173 882 2005 2748 181 Cuts at: 9701 3021 Size: 5717 Fragments arranged by size: 5717 2748 2005 882 634 181 173 37 20 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8907 11404 809 Size: 507 7591 2497 1802 Fragments arranged by size: 7591 2497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3347 7369 8028 Size: 54 1425 50 275 18 1517 4022 659 Cuts at: 8028 9844 10733 12362 8 Size: 1816 889 1629 43 Fragments arranged by size: 4022 1816 1629 1517 1425 889 659 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7369 9844 1537 Size: 275 5557 2475 4090 Fragments arranged by size: 5557 4090 2475 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 3387 3442 3517 4656 4918 5131 5598 Size: 978 1056 55 75 1139 262 213 467 Cuts at: 5598 5757 6009 7392 7755 7947 8263 10327 1353 Size: 159 252 1383 363 192 316 2064 3423 Fragments arranged by size: 3423 2064 1383 1139 1056 978 467 363 316 262 252 213 192 159 75 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2562 3223 10984 11358 11504 387 Size: 1064 138 973 661 7761 374 146 1280 Fragments arranged by size: 7761 1280 1064 973 661 374 146 138 EcoICRI GAG'CTC Cuts at: 3066 3315 5767 6533 3066 Size: 249 2452 766 8930 Fragments arranged by size: 8930 2452 766 249 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2218 2298 2312 2967 3552 3831 Size: 393 462 124 80 14 655 585 279 Cuts at: 3831 4431 4446 4637 4704 5186 6068 6251 6349 Size: 600 15 191 67 482 882 183 98 Cuts at: 6349 6851 6960 7325 7376 8128 8743 8972 9376 Size: 502 109 365 51 752 615 229 404 Cuts at: 9376 9531 9587 9614 10460 11057 11844 12112 1239 Size: 155 56 27 846 597 787 268 1524 Fragments arranged by size: 1524 882 846 787 752 655 615 600 597 585 502 482 462 404 393 365 279 268 229 191 183 155 124 109 98 80 67 56 51 27 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 3262 4818 8063 3262 Size: 1556 3245 7596 Fragments arranged by size: 7596 3245 1556 EcoO109I rG'GnC_Cy Cuts at: 6209 6342 6370 9589 6209 Size: 133 28 3219 9017 Fragments arranged by size: 9017 3219 133 28 EcoRI G'AATT_C Cuts at: 4903 5227 8076 9706 10084 4903 Size: 324 2849 1630 378 7216 Fragments arranged by size: 7216 2849 1630 378 324 EcoRV GAT'ATC Cuts at: 6196 6196 Size: 12397 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6581 7745 6581 Size: 1164 11233 FspI TGC'GCA Cuts at: 10903 10903 Size: 12397 HaeII r_GCGC'y Cuts at: 1678 11544 1678 Size: 9866 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 2765 2797 2822 Size: 32 135 32 788 32 373 32 25 Cuts at: 2822 2854 3953 3985 4700 4732 4923 4955 5519 Size: 32 1099 32 715 32 191 32 564 Cuts at: 5519 5551 5915 5947 6083 6115 7077 7109 7532 Size: 32 364 32 136 32 962 32 423 Cuts at: 7532 7564 7949 7981 9109 9141 11038 11070 11112 Size: 32 385 32 1128 32 1897 32 42 Cuts at: 11112 11144 1373 Size: 32 2626 Fragments arranged by size: 2626 1897 1128 1099 962 788 715 564 423 385 373 364 191 136 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 6990 9741 9987 669 Size: 988 281 5052 2751 246 3079 Fragments arranged by size: 5052 3079 2751 988 281 246 HindIII A'AGCT_T Cuts at: 748 7388 748 Size: 6640 5757 HpaI GTT'AAC Cuts at: 6990 9987 6990 Size: 2997 9400 KpnI G_GTAC'C Cuts at: 9722 9722 Size: 12397 MfeI C'AATT_G Cuts at: 7054 8958 9996 7054 Size: 1904 1038 9455 Fragments arranged by size: 9455 1904 1038 MluI A'CGCG_T Cuts at: 12273 12273 Size: 12397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3646 7428 8030 8936 9353 11511 11695 1305 Size: 2341 3782 602 906 417 2158 184 2007 Fragments arranged by size: 3782 2341 2158 2007 906 602 417 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 8030 10 Size: 54 1425 343 6198 4377 Fragments arranged by size: 6198 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3285 4289 4605 5062 5340 5526 5742 Size: 648 2119 1004 316 457 278 186 216 Cuts at: 5742 6379 6654 6894 10355 10714 10873 518 Size: 637 275 240 3461 359 159 2042 Fragments arranged by size: 3461 2119 2042 1004 648 637 457 359 316 278 275 240 216 186 159 MspA1I CmG'CkG Cuts at: 1606 2014 2318 6894 7801 9813 10490 11638 11883 Size: 408 304 4576 907 2012 677 1148 245 Cuts at: 11883 1606 Size: 2120 Fragments arranged by size: 4576 2120 2012 1148 907 677 408 304 245 NaeI GCC'GGC Cuts at: 8784 8784 Size: 12397 NcoI C'CATG_G Cuts at: 513 1065 5028 5241 513 Size: 552 3963 213 7669 Fragments arranged by size: 7669 3963 552 213 NdeI CA'TA_TG Cuts at: 387 9334 387 Size: 8947 3450 NgoMIV G'CCGG_C Cuts at: 8782 8782 Size: 12397 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12397 NotI GC'GGCC_GC Cuts at: 9844 9844 Size: 12397 NsiI A_TGCA'T Cuts at: 4148 5059 4148 Size: 911 11486 NspI r_CATG'y Cuts at: 1132 1229 4146 4150 4216 4551 4775 4973 5741 Size: 97 2917 4 66 335 224 198 768 Cuts at: 5741 6552 6653 8690 8910 9649 9755 11300 1132 Size: 811 101 2037 220 739 106 1545 2229 Fragments arranged by size: 2917 2229 2037 1545 811 768 739 335 224 220 198 106 101 97 66 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 12397 PciI A'CATG_T Cuts at: 4969 5737 8686 8906 11296 4969 Size: 768 2949 220 2390 6070 Fragments arranged by size: 6070 2949 2390 768 220 PflMI CCAn_nnn'nTGG Cuts at: 1108 8725 1108 Size: 7617 4780 PfoI T'CCnGG_A Cuts at: 5944 8040 5944 Size: 2096 10301 PmeI GTTT'AAAC Cuts at: 9701 9701 Size: 12397 PmlI CAC'GTG Cuts at: 5894 5894 Size: 12397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7274 7306 10466 10498 11979 12011 7274 Size: 32 3160 32 1481 32 7660 Fragments arranged by size: 7660 3160 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6370 9589 6370 Size: 3219 9178 PshAI GACnn'nnGTC Cuts at: 1613 3628 7502 1613 Size: 2015 3874 6508 Fragments arranged by size: 6508 3874 2015 PsiI TTA'TAA Cuts at: 2735 3669 3926 7406 7655 8475 9967 2735 Size: 934 257 3480 249 820 1492 5165 Fragments arranged by size: 5165 3480 1492 934 820 257 249 PspOMI G'GGCC_C Cuts at: 6341 6341 Size: 12397 PspXI vC'TCGA_Gb Cuts at: 6777 6777 Size: 12397 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4567 4599 4738 4770 4567 Size: 32 139 32 12194 Fragments arranged by size: 12194 139 32 32 PstI C_TGCA'G Cuts at: 830 2019 2386 9749 830 Size: 1189 367 7363 3478 Fragments arranged by size: 7363 3478 1189 367 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12397 PvuII CAG'CTG Cuts at: 2014 2318 6894 7801 2014 Size: 304 4576 907 6610 Fragments arranged by size: 6610 4576 907 304 SacI G_AGCT'C Cuts at: 3068 3317 5769 6535 3068 Size: 249 2452 766 8930 Fragments arranged by size: 8930 2452 766 249 SalI G'TCGA_C Cuts at: 1936 9739 1936 Size: 7803 4594 SanDI GG'GwC_CC Cuts at: 6370 6370 Size: 12397 SapI GCTCTTCn'nnn_ Cuts at: 5131 5598 5757 5131 Size: 467 159 11771 Fragments arranged by size: 11771 467 159 SbfI CC_TGCA'GG Cuts at: 9749 9749 Size: 12397 ScaI AGT'ACT Cuts at: 7464 8861 9572 10645 12357 7464 Size: 1397 711 1073 1712 7504 Fragments arranged by size: 7504 1712 1397 1073 711 SexAI A'CCwGG_T Cuts at: 7203 8509 7203 Size: 1306 11091 SfcI C'TryA_G Cuts at: 826 1045 2015 2382 2550 5100 7752 8355 8643 Size: 219 970 367 168 2550 2652 603 288 Cuts at: 8643 9745 10880 11561 11752 826 Size: 1102 1135 681 191 1471 Fragments arranged by size: 2652 2550 1471 1135 1102 970 681 603 367 288 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12397 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12397 SmaI CCC'GGG Cuts at: 1872 9724 1872 Size: 7852 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3412 4890 5793 5973 6534 6694 Size: 197 926 1469 1478 903 180 561 160 Cuts at: 6694 6777 8877 9198 9296 10501 11402 11664 820 Size: 83 2100 321 98 1205 901 262 1553 Fragments arranged by size: 2100 1553 1478 1469 1205 926 903 901 561 321 262 197 180 160 98 83 SnaBI TAC'GTA Cuts at: 493 10188 493 Size: 9695 2702 SpeI A'CTAG_T Cuts at: 152 4580 152 Size: 4428 7969 SphI G_CATG'C Cuts at: 1132 4146 4150 4216 4775 6653 9755 1132 Size: 3014 4 66 559 1878 3102 3774 Fragments arranged by size: 3774 3102 3014 1878 559 66 4 SspI AAT'ATT Cuts at: 5 52 2814 3134 4174 4438 6740 10321 5 Size: 47 2762 320 1040 264 2302 3581 2081 Fragments arranged by size: 3581 2762 2302 2081 1040 320 264 47 StuI AGG'CCT Cuts at: 1517 8528 9356 1517 Size: 7011 828 4558 Fragments arranged by size: 7011 4558 828 StyI C'CwwG_G Cuts at: 513 1065 5028 5241 8628 9624 9836 513 Size: 552 3963 213 3387 996 212 3074 Fragments arranged by size: 3963 3387 3074 996 552 213 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5568 8593 10424 10441 10594 10779 11224 Size: 1255 3988 3025 1831 17 153 185 445 Cuts at: 11224 325 Size: 1498 Fragments arranged by size: 3988 3025 1831 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 4388 5513 Size: 274 80 33 51 1443 1751 660 1125 Cuts at: 5513 6506 7462 8746 8848 8859 8962 9570 9630 Size: 993 956 1284 102 11 103 608 60 Cuts at: 9630 9642 10643 12355 96 Size: 12 1001 1712 138 Fragments arranged by size: 1751 1712 1443 1284 1125 1001 993 956 660 608 274 138 103 102 80 60 51 33 12 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4662 4694 4956 4988 6376 6408 7280 7312 7564 Size: 32 262 32 1388 32 872 32 252 Cuts at: 7564 7596 7603 7635 8579 8611 8741 8773 9007 Size: 32 7 32 944 32 130 32 234 Cuts at: 9007 9039 9581 9613 12357 12389 4662 Size: 32 542 32 2744 32 4670 Fragments arranged by size: 4670 2744 1388 944 872 542 262 252 234 130 32 32 32 32 32 32 32 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 12397 XbaI T'CTAG_A Cuts at: 3242 9733 3242 Size: 6491 5906 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 5649 8038 8581 2470 Size: 3179 2389 543 6286 Fragments arranged by size: 6286 3179 2389 543 XhoI C'TCGA_G Cuts at: 1943 6777 9198 1943 Size: 4834 2421 5142 Fragments arranged by size: 5142 4834 2421 XmaI C'CCGG_G Cuts at: 1870 9722 1870 Size: 7852 4545 XmnI GAAnn'nnTTC Cuts at: 2877 7037 10526 2877 Size: 4160 3489 4748 Fragments arranged by size: 4748 4160 3489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10205 276 Size: 53 83 186 936 8671 2468 Fragments arranged by size: 8671 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI PacI RsrII SacII SfiI SfoI SrfI SwaI