################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AccII AccIII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlnI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FseI FspI HaeII HapII HgaI HpaI HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 20 enzymes) # ################################### AatII 4100 GACGTC GACGT^C AcyI 4100 GACGTC G[GA]^CG[CT]C BsaAI 2720 TACGTG [CT]AC^GT[GA] BsaBI 4225 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4100 GACGTC G[GA]^CG[CT]C BstAPI 460 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 2979 GCGC GCG^C DrdI 534 GACTGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 4409 CCATGG C^C[GA][CT]GG EcoNI 5233 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1305 GATATC GAT^ATC HhaI 2979 GCGC GCG^C Hin1I 4100 GACGTC G[GA]^CG[CT]C HinP1I 2979 GCGC G^CGC MamI 4225 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 4409 CCATGG C^CATGG PshAI 4606 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SpeI 1315 ACTAGT A^CTAGT StuI 1976 AGGCCT AGG^CCT XcmI 4388 CCATGAATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AclI 69 AACGTT AA^CGTT 2706 AACGTT AlwNI 3704 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 5432 CAGCTGCTG AspI 3267 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5180 GACCTGGTC BalI 4356 TGGCCA TGG^CCA 5108 TGGCCA BanII 2224 GAGCTC G[GA]GC[CT]^C 3054 GAGCTC BclI 290 TGATCA T^GATCA 3467 TGATCA BseRI 3059 CTCCTC GAGGAG(10/8) 4160 CTCCTC BsrBI 4269 CCGCTC CCGCTC(-3/-3) 649 GAGCGG BssSI 1109 CTCGTG CACGAG(-5/-1) 5324 CTCGTG EcoRI 431 GAATTC G^AATTC 2085 GAATTC FbaI 290 TGATCA T^GATCA 3467 TGATCA MluNI 4356 TGGCCA TGG^CCA 5108 TGGCCA MscI 4356 TGGCCA TGG^CCA 5108 TGGCCA PflFI 3267 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5180 GACCTGGTC Psp1406I 69 AACGTT AA^CGTT 2706 AACGTT PvuII 2434 CAGCTG CAG^CTG 5432 CAGCTG SacI 2224 GAGCTC GAGCT^C 3054 GAGCTC SalI 734 GTCGAC G^TCGAC 4913 GTCGAC SapI 646 GAAGAGC GCTCTTC(1/4) 1429 GAAGAGC Tth111I 3267 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5180 GACCTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraIII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI