(Circular) (Six-base) MAPSORT of: pFN21AE3492.seq Check: 2508 from: 1 to: 10234 pFN21AE3492 10234 bp With 182 enzymes: SgfI * June 11, 2014 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6737 6737 Size: 10234 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6109 8044 278 Size: 53 83 186 936 4573 1935 2468 Fragments arranged by size: 4573 2468 1935 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2740 6919 7408 7577 1937 Size: 803 4179 489 169 4594 Fragments arranged by size: 4594 4179 803 489 169 Acc65I G'GTAC_C Cuts at: 7555 7555 Size: 10234 AclI AA'CG_TT Cuts at: 1563 2075 4712 8361 8734 1563 Size: 512 2637 3649 373 3063 Fragments arranged by size: 3649 3063 2637 512 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 3551 3906 5231 5246 5309 5502 5576 Size: 1038 881 355 1325 15 63 193 74 Cuts at: 5576 5808 6284 6318 6547 7224 8297 9681 9949 Size: 232 476 34 229 677 1073 1384 268 Cuts at: 9949 1632 Size: 1917 Fragments arranged by size: 1917 1384 1325 1073 1038 881 677 476 355 268 232 229 193 74 63 34 15 AflII C'TTAA_G Cuts at: 820 1017 3712 4214 4898 820 Size: 197 2695 502 684 6156 Fragments arranged by size: 6156 2695 684 502 197 AflIII A'CryG_T Cuts at: 1203 9133 10110 1203 Size: 7930 977 1327 Fragments arranged by size: 7930 1327 977 AgeI A'CCGG_T Cuts at: 9065 9065 Size: 10234 AhdI GACnn_n'nnGTC Cuts at: 8963 8963 Size: 10234 AleI CACnn'nnGTG Cuts at: 1166 6786 1166 Size: 5620 4614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10234 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6642 6674 6642 Size: 32 10202 AlwNI CAG_nnn'CTG Cuts at: 5714 7442 7977 9549 5714 Size: 1728 535 1572 6399 Fragments arranged by size: 6399 1728 1572 535 ApaLI G'TGCA_C Cuts at: 8291 9447 8291 Size: 1156 9078 ApoI r'AATT_y Cuts at: 1493 2016 2264 2423 2436 2893 3023 3580 3646 Size: 523 248 159 13 457 130 557 66 Cuts at: 3646 3774 3824 3997 4090 4585 5335 5812 5867 Size: 128 50 173 93 495 750 477 55 Cuts at: 5867 5959 5994 6149 6451 6794 7498 7543 7769 Size: 92 35 155 302 343 704 45 226 Cuts at: 7769 7921 10030 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 750 704 557 523 495 477 457 343 302 248 226 173 159 155 152 130 128 93 92 66 55 50 45 35 13 AseI AT'TA_AT Cuts at: 160 1032 2169 3227 5147 6005 6234 8788 160 Size: 872 1137 1058 1920 858 229 2554 1606 Fragments arranged by size: 2554 1920 1606 1137 1058 872 858 229 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10234 AvaI C'yCGr_G Cuts at: 1870 1943 7559 1870 Size: 73 5616 4545 Fragments arranged by size: 5616 4545 73 BamHI G'GATC_C Cuts at: 7564 7931 7564 Size: 367 9867 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7555 9010 618 Size: 325 217 627 5768 1455 1842 Fragments arranged by size: 5768 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4233 5063 7553 1391 Size: 2842 830 2490 4072 Fragments arranged by size: 4072 2842 2490 830 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 3102 3557 3699 4760 5088 5394 Size: 971 1164 39 455 142 1061 328 306 Cuts at: 5394 5451 5607 5816 6615 928 Size: 57 156 209 799 4547 Fragments arranged by size: 4547 1164 1061 971 799 455 328 306 209 156 142 57 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8425 8459 1903 Size: 34 6488 34 3678 Fragments arranged by size: 6488 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8126 9336 8126 Size: 1210 9024 BclI T'GATC_A Cuts at: 1548 2295 5472 1548 Size: 747 3177 6310 Fragments arranged by size: 6310 3177 747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8845 136 Size: 107 122 71 631 605 7173 1525 Fragments arranged by size: 7173 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 10229 10229 Size: 10234 BlpI GC'TnA_GC Cuts at: 3612 3812 4167 5942 7651 3612 Size: 200 355 1775 1709 6195 Fragments arranged by size: 6195 1775 1709 355 200 Bme1580I G_kGCm'C Cuts at: 1790 8295 9451 1790 Size: 6505 1156 2573 Fragments arranged by size: 6505 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6875 7965 8923 Size: 416 53 83 399 24 5458 1090 958 Cuts at: 8923 442 Size: 1753 Fragments arranged by size: 5458 1753 1090 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10234 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5082 5082 Size: 10234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2700 6268 6872 6932 8894 1239 Size: 815 646 3568 604 60 1962 2579 Fragments arranged by size: 3568 2579 1962 815 646 604 60 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 10234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 4820 5240 5597 7006 7236 8359 9224 9522 Size: 1953 420 357 1409 230 1123 865 298 Cuts at: 9522 2867 Size: 3579 Fragments arranged by size: 3579 1953 1409 1123 865 420 357 298 230 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 5586 7177 8897 882 Size: 617 1170 2917 1591 1720 2219 Fragments arranged by size: 2917 2219 1720 1591 1170 617 BsaAI yAC'GTr Cuts at: 493 1204 4727 8025 493 Size: 711 3523 3298 2702 Fragments arranged by size: 3523 3298 2702 711 BsaBI GATnn'nnATC Cuts at: 6234 10228 6234 Size: 3994 6240 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6106 8041 8423 9058 Size: 53 83 186 936 4573 1935 382 635 Cuts at: 9058 275 Size: 1451 Fragments arranged by size: 4573 1935 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8667 9065 9339 9486 9897 8667 Size: 398 274 147 411 9004 Fragments arranged by size: 9004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 10009 Fragments arranged by size: 10009 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5055 6156 1186 Size: 3869 1101 5264 Fragments arranged by size: 5264 3869 1101 BseYI C'CCAG_C Cuts at: 3294 9437 9933 3294 Size: 6143 496 3595 Fragments arranged by size: 6143 3595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2418 2679 3756 6428 6771 1163 Size: 573 682 261 1077 2672 343 4626 Fragments arranged by size: 4626 2672 1077 682 573 343 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7684 8445 9473 1540 Size: 275 186 5683 761 1028 2301 Fragments arranged by size: 5683 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3121 4233 5063 6888 8295 8380 9451 3121 Size: 1112 830 1825 1407 85 1071 3904 Fragments arranged by size: 3904 1825 1407 1112 1071 830 85 BsmI GAATG_Cn' Cuts at: 2235 5320 5383 7745 7838 2235 Size: 3085 63 2362 93 4631 Fragments arranged by size: 4631 3085 2362 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3121 4233 5063 6888 7553 8295 8380 Size: 399 1331 1112 830 1825 665 742 85 Cuts at: 8380 9451 1391 Size: 1071 2174 Fragments arranged by size: 2174 1825 1331 1112 1071 830 742 665 399 85 BspEI T'CCGG_A Cuts at: 9897 9897 Size: 10234 BspHI T'CATG_A Cuts at: 2043 2298 3178 3781 4792 5367 5482 5503 5701 Size: 255 880 603 1011 575 115 21 198 Cuts at: 5701 6808 8123 9853 2043 Size: 1107 1315 1730 2424 Fragments arranged by size: 2424 1730 1315 1107 1011 880 603 575 255 198 115 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 6737 7500 7589 844 Size: 1685 4208 763 89 3489 Fragments arranged by size: 4208 3489 1685 763 89 BsrBI CCG'CTC Cuts at: 1394 1823 2656 6276 8121 1394 Size: 429 833 3620 1845 3507 Fragments arranged by size: 3620 3507 1845 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2531 2585 3475 4388 8729 8903 65 Size: 1183 1283 54 890 913 4341 174 1396 Fragments arranged by size: 4341 1396 1283 1183 913 890 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 8878 9065 1700 Size: 110 7068 187 2869 Fragments arranged by size: 7068 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10234 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10234 BssSI C'ACGA_G Cuts at: 3114 7329 8294 9306 3114 Size: 4215 965 1012 4042 Fragments arranged by size: 4215 4042 1012 965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2471 794 Size: 1677 8557 BstBI TT'CG_AA Cuts at: 9038 9038 Size: 10234 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10234 BstXI CCAn_nnnn'nTGG Cuts at: 2056 6577 6788 2056 Size: 4521 211 5502 Fragments arranged by size: 5502 4521 211 BstYI r'GATC_y Cuts at: 1972 3592 6052 6566 7564 7931 8316 8333 9785 Size: 1620 2460 514 998 367 385 17 1452 Cuts at: 9785 9871 9883 10229 1972 Size: 86 12 346 1977 Fragments arranged by size: 2460 1977 1620 1452 998 514 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 6414 513 Size: 552 939 4410 4333 Fragments arranged by size: 4410 4333 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9495 Fragments arranged by size: 9495 683 56 BtsI GCAGTG_nn' Cuts at: 5174 6793 6838 7458 7750 8544 8564 5174 Size: 1619 45 620 292 794 20 6844 Fragments arranged by size: 6844 1619 794 620 292 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10199 DraI TTT'AAA Cuts at: 3957 4051 5778 7538 3957 Size: 94 1727 1760 6653 Fragments arranged by size: 6653 1760 1727 94 DraIII CAC_nnn'GTG Cuts at: 2708 4727 6626 2708 Size: 2019 1899 6316 Fragments arranged by size: 6316 2019 1899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2545 9241 809 Size: 507 1229 6696 1802 Fragments arranged by size: 6696 1802 1229 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 6361 7113 Size: 54 1425 50 275 18 258 4273 752 Cuts at: 7113 7681 8570 10199 8 Size: 568 889 1629 43 Fragments arranged by size: 4273 1629 1425 889 752 568 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7681 1537 Size: 275 5869 4090 Fragments arranged by size: 5869 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 2692 3429 5307 6120 6556 7075 8164 Size: 1293 46 737 1878 813 436 519 1089 Cuts at: 8164 1353 Size: 3423 Fragments arranged by size: 3423 1878 1293 1089 813 737 519 436 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8821 9195 9341 387 Size: 1064 138 7232 374 146 1280 Fragments arranged by size: 7232 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4231 5061 4231 Size: 830 9404 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2670 2700 3551 3906 5231 5246 Size: 393 422 616 30 851 355 1325 15 Cuts at: 5246 5309 5502 5576 5808 6268 6284 6318 6547 Size: 63 193 74 232 460 16 34 229 Cuts at: 6547 6872 6932 7224 8297 8894 9681 9949 1239 Size: 325 60 292 1073 597 787 268 1524 Fragments arranged by size: 1524 1325 1073 851 787 616 597 460 422 393 355 325 292 268 232 229 193 74 63 60 34 30 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 7242 7242 Size: 10234 EcoRI G'AATT_C Cuts at: 2436 4090 7543 7921 2436 Size: 1654 3453 378 4749 Fragments arranged by size: 4749 3453 1654 378 EcoRV GAT'ATC Cuts at: 3312 3312 Size: 10234 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3191 3632 4229 6314 7052 3191 Size: 441 597 2085 738 6373 Fragments arranged by size: 6373 2085 738 597 441 FspI TGC'GCA Cuts at: 8740 8740 Size: 10234 HaeII r_GCGC'y Cuts at: 1678 9381 1678 Size: 7703 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 3362 3394 3426 Size: 32 135 32 1325 32 433 32 32 Cuts at: 3426 4366 4398 5438 5464 5470 5496 5513 5545 Size: 940 32 1040 26 6 26 17 32 Cuts at: 5545 5894 5926 6584 6616 6809 6841 6907 6939 Size: 349 32 658 32 193 32 66 32 Cuts at: 6939 7174 7206 8875 8907 8949 8981 1373 Size: 235 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 1325 1040 940 658 433 349 235 193 135 66 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 26 26 17 6 HincII GTy'rAC Cuts at: 669 1657 1938 2741 3375 5771 6547 6920 7578 Size: 988 281 803 634 2396 776 373 658 Cuts at: 7578 7824 669 Size: 246 3079 Fragments arranged by size: 3079 2396 988 803 776 658 634 373 281 246 HindIII A'AGCT_T Cuts at: 748 2975 5321 6995 748 Size: 2227 2346 1674 3987 Fragments arranged by size: 3987 2346 2227 1674 HpaI GTT'AAC Cuts at: 7824 7824 Size: 10234 KpnI G_GTAC'C Cuts at: 7559 7559 Size: 10234 MfeI C'AATT_G Cuts at: 2291 4502 4979 5897 7833 2291 Size: 2211 477 918 1936 4692 Fragments arranged by size: 4692 2211 1936 918 477 MluI A'CGCG_T Cuts at: 10110 10110 Size: 10234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 3072 4552 5619 9348 9532 1305 Size: 1503 264 1480 1067 3729 184 2007 Fragments arranged by size: 3729 2007 1503 1480 1067 264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6363 7115 10 Size: 54 1425 343 4531 752 3129 Fragments arranged by size: 4531 3129 1425 752 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 4160 6786 6813 8192 8551 8710 Size: 648 1083 1911 2626 27 1379 359 159 Cuts at: 8710 518 Size: 2042 Fragments arranged by size: 2626 2042 1911 1379 1083 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2468 3721 4441 7439 7650 8327 9475 9720 Size: 862 1253 720 2998 211 677 1148 245 Cuts at: 9720 1606 Size: 2120 Fragments arranged by size: 2998 2120 1253 1148 862 720 677 245 211 NcoI C'CATG_G Cuts at: 513 1065 2004 6414 513 Size: 552 939 4410 4333 Fragments arranged by size: 4410 4333 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10234 NotI GC'GGCC_GC Cuts at: 7681 7681 Size: 10234 NspI r_CATG'y Cuts at: 1132 1229 7592 9137 1132 Size: 97 6363 1545 2229 Fragments arranged by size: 6363 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3352 7139 1348 Size: 2004 3787 4443 Fragments arranged by size: 4443 3787 2004 PciI A'CATG_T Cuts at: 9133 9133 Size: 10234 PflMI CCAn_nnn'nTGG Cuts at: 1108 5075 5899 6370 1108 Size: 3967 824 471 4972 Fragments arranged by size: 4972 3967 824 471 PmeI GTTT'AAAC Cuts at: 7538 7538 Size: 10234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3103 3135 8303 8335 9816 9848 3103 Size: 32 5168 32 1481 32 3489 Fragments arranged by size: 5168 3489 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 6615 1613 Size: 5002 5232 PsiI TTA'TAA Cuts at: 3030 3864 4971 7804 3030 Size: 834 1107 2833 5460 Fragments arranged by size: 5460 2833 1107 834 PstI C_TGCA'G Cuts at: 830 5719 6559 7465 7586 830 Size: 4889 840 906 121 3478 Fragments arranged by size: 4889 3478 906 840 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10234 PvuII CAG'CTG Cuts at: 4441 7439 4441 Size: 2998 7236 SacI G_AGCT'C Cuts at: 4233 5063 4233 Size: 830 9404 SalI G'TCGA_C Cuts at: 1936 2739 6918 7576 1936 Size: 803 4179 658 4594 Fragments arranged by size: 4594 4179 803 658 SapI GCTCTTCn'nnn_ Cuts at: 2646 3429 2646 Size: 783 9451 SbfI CC_TGCA'GG Cuts at: 7586 7586 Size: 10234 ScaI AGT'ACT Cuts at: 4849 6445 6854 8482 10194 4849 Size: 1596 409 1628 1712 4889 Fragments arranged by size: 4889 1712 1628 1596 409 SexAI A'CCwGG_T Cuts at: 2151 6402 7186 2151 Size: 4251 784 5199 Fragments arranged by size: 5199 4251 784 SfcI C'TryA_G Cuts at: 826 1045 3459 4172 5715 5764 6011 6522 6555 Size: 219 2414 713 1543 49 247 511 33 Cuts at: 6555 7461 7582 8717 9398 9589 826 Size: 906 121 1135 681 191 1471 Fragments arranged by size: 2414 1543 1471 1135 906 713 681 511 247 219 191 121 49 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10234 SmaI CCC'GGG Cuts at: 1872 7561 1872 Size: 5689 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 3712 4214 4799 4898 5219 Size: 197 926 903 866 502 585 99 321 Cuts at: 5219 5576 7021 7251 8338 9239 9501 820 Size: 357 1445 230 1087 901 262 1553 Fragments arranged by size: 1553 1445 1087 926 903 901 866 585 502 357 321 262 230 197 99 SnaBI TAC'GTA Cuts at: 493 8025 493 Size: 7532 2702 SpeI A'CTAG_T Cuts at: 152 3320 152 Size: 3168 7066 SphI G_CATG'C Cuts at: 1132 7592 1132 Size: 6460 3774 SspI AAT'ATT Cuts at: 5 52 8158 5 Size: 47 8106 2081 Fragments arranged by size: 8106 2081 47 StuI AGG'CCT Cuts at: 1517 3983 1517 Size: 2466 7768 StyI C'CwwG_G Cuts at: 513 1065 2004 6369 6414 7398 7673 513 Size: 552 939 4365 45 984 275 3074 Fragments arranged by size: 4365 3074 984 939 552 275 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6283 8261 8278 8431 8616 9061 325 Size: 1255 4703 1978 17 153 185 445 1498 Fragments arranged by size: 4703 1978 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4847 6078 6443 Size: 274 80 33 51 1443 2870 1231 365 Cuts at: 6443 6852 7006 7483 8480 10192 96 Size: 409 154 477 997 1712 138 Fragments arranged by size: 2870 1712 1443 1231 997 477 409 365 274 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 10194 10226 2225 Size: 32 7937 32 2233 Fragments arranged by size: 7937 2233 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5275 7188 1316 Size: 3959 1913 4362 Fragments arranged by size: 4362 3959 1913 XbaI T'CTAG_A Cuts at: 7570 7570 Size: 10234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6400 6400 Size: 10234 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10234 XmaI C'CCGG_G Cuts at: 1870 7559 1870 Size: 5689 4545 XmnI GAAnn'nnTTC Cuts at: 2436 3438 5499 6294 8363 2436 Size: 1002 2061 795 2069 4307 Fragments arranged by size: 4307 2069 2061 1002 795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6107 8042 276 Size: 53 83 186 936 4573 1935 2468 Fragments arranged by size: 4573 2468 1935 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI