(Linear) (Six-base) MAPSORT of: KIEE3492.seq Check: 9729 from: 1 to: 5529 KIEE3492.orf With 182 enzymes: SgfI * June 11, 2014 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4732 5529 Size: 4732 797 AatII G_ACGT'C Cuts at: 0 4104 5529 Size: 4104 1425 AccI GT'mk_AC Cuts at: 0 735 4914 5403 5529 Size: 735 4179 489 126 Fragments arranged by size: 4179 735 489 126 AclI AA'CG_TT Cuts at: 0 70 2707 5529 Size: 70 2637 2822 Fragments arranged by size: 2822 2637 70 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 1546 1901 3226 3241 3304 3497 3571 Size: 665 881 355 1325 15 63 193 74 Cuts at: 3571 3803 4279 4313 4542 5219 5529 Size: 232 476 34 229 677 310 Fragments arranged by size: 1325 881 677 665 476 355 310 232 229 193 74 63 34 15 AflII C'TTAA_G Cuts at: 0 1707 2209 2893 5529 Size: 1707 502 684 2636 Fragments arranged by size: 2636 1707 684 502 AleI CACnn'nnGTG Cuts at: 0 4781 5529 Size: 4781 748 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4637 4669 5529 Size: 4637 32 860 Fragments arranged by size: 4637 860 32 AlwNI CAG_nnn'CTG Cuts at: 0 3709 5437 5529 Size: 3709 1728 92 Fragments arranged by size: 3709 1728 92 ApoI r'AATT_y Cuts at: 0 11 259 418 431 888 1018 1575 1641 Size: 11 248 159 13 457 130 557 66 Cuts at: 1641 1769 1819 1992 2085 2580 3330 3807 3862 Size: 128 50 173 93 495 750 477 55 Cuts at: 3862 3954 3989 4144 4446 4789 5493 5529 Size: 92 35 155 302 343 704 36 Fragments arranged by size: 750 704 557 495 477 457 343 302 248 173 159 155 130 128 93 92 66 55 50 36 35 13 11 AseI AT'TA_AT Cuts at: 0 164 1222 3142 4000 4229 5529 Size: 164 1058 1920 858 229 1300 Fragments arranged by size: 1920 1300 1058 858 229 164 BanII G_rGCy'C Cuts at: 0 2228 3058 5529 Size: 2228 830 2471 Fragments arranged by size: 2471 2228 830 BbsI GAAGACnn'nnnn_ Cuts at: 0 1058 1097 1552 1694 2755 3083 3389 3446 Size: 1058 39 455 142 1061 328 306 57 Cuts at: 3446 3602 3811 4610 5529 Size: 156 209 799 919 Fragments arranged by size: 1061 1058 919 799 455 328 306 209 156 142 57 39 BclI T'GATC_A Cuts at: 0 290 3467 5529 Size: 290 3177 2062 Fragments arranged by size: 3177 2062 290 BlpI GC'TnA_GC Cuts at: 0 1607 1807 2162 3937 5529 Size: 1607 200 355 1775 1592 Fragments arranged by size: 1775 1607 1592 355 200 BmrI ACTGGGnnnn_n' Cuts at: 0 4870 5529 Size: 4870 659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3077 5529 Size: 3077 2452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 695 4263 4867 4927 5529 Size: 49 646 3568 604 60 602 Fragments arranged by size: 3568 646 604 602 60 49 Bpu10I CC'TnA_GC Cuts at: 0 787 5529 Size: 787 4742 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 2815 3235 3592 5001 5231 5529 Size: 862 1953 420 357 1409 230 298 Fragments arranged by size: 1953 1409 862 420 357 298 230 BsaI GGTCTCn'nnnn_ Cuts at: 0 664 3581 5172 5529 Size: 664 2917 1591 357 Fragments arranged by size: 2917 1591 664 357 BsaAI yAC'GTr Cuts at: 0 2722 5529 Size: 2722 2807 BsaBI GATnn'nnATC Cuts at: 0 4229 5529 Size: 4229 1300 BsaHI Gr'CG_yC Cuts at: 0 4101 5529 Size: 4101 1428 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3050 4151 5529 Size: 3050 1101 1378 Fragments arranged by size: 3050 1378 1101 BseYI C'CCAG_C Cuts at: 0 1289 5529 Size: 1289 4240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 413 674 1751 4423 4766 5529 Size: 413 261 1077 2672 343 763 Fragments arranged by size: 2672 1077 763 413 343 261 BsiHKAI G_wGCw'C Cuts at: 0 1116 2228 3058 4883 5529 Size: 1116 1112 830 1825 646 Fragments arranged by size: 1825 1116 1112 830 646 BsmI GAATG_Cn' Cuts at: 0 230 3315 3378 5529 Size: 230 3085 63 2151 Fragments arranged by size: 3085 2151 230 63 Bsp1286I G_dGCh'C Cuts at: 0 1116 2228 3058 4883 5529 Size: 1116 1112 830 1825 646 Fragments arranged by size: 1825 1116 1112 830 646 BspHI T'CATG_A Cuts at: 0 38 293 1173 1776 2787 3362 3477 3498 Size: 38 255 880 603 1011 575 115 21 Cuts at: 3498 3696 4803 5529 Size: 198 1107 726 Fragments arranged by size: 1107 1011 880 726 603 575 255 198 115 38 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 524 4732 5495 5529 Size: 524 4208 763 34 Fragments arranged by size: 4208 763 524 34 BsrBI CCG'CTC Cuts at: 0 651 4271 5529 Size: 651 3620 1258 Fragments arranged by size: 3620 1258 651 BsrDI GCAATG_nn' Cuts at: 0 526 580 1470 2383 5529 Size: 526 54 890 913 3146 Fragments arranged by size: 3146 913 890 526 54 BssSI C'ACGA_G Cuts at: 0 1109 5324 5529 Size: 1109 4215 205 Fragments arranged by size: 4215 1109 205 BstAPI GCAn_nnn'nTGC Cuts at: 0 466 5529 Size: 466 5063 BstXI CCAn_nnnn'nTGG Cuts at: 0 51 4572 4783 5529 Size: 51 4521 211 746 Fragments arranged by size: 4521 746 211 51 BstYI r'GATC_y Cuts at: 0 1587 4047 4561 5529 Size: 1587 2460 514 968 Fragments arranged by size: 2460 1587 968 514 BtgI C'CryG_G Cuts at: 0 4409 5529 Size: 4409 1120 BtsI GCAGTG_nn' Cuts at: 0 3169 4788 4833 5453 5529 Size: 3169 1619 45 620 76 Fragments arranged by size: 3169 1619 620 76 45 DraI TTT'AAA Cuts at: 0 1952 2046 3773 5529 Size: 1952 94 1727 1756 Fragments arranged by size: 1952 1756 1727 94 DraIII CAC_nnn'GTG Cuts at: 0 703 2722 4621 5529 Size: 703 2019 1899 908 Fragments arranged by size: 2019 1899 908 703 DrdI GACnn_nn'nnGTC Cuts at: 0 540 5529 Size: 540 4989 EaeI y'GGCC_r Cuts at: 0 83 4356 5108 5529 Size: 83 4273 752 421 Fragments arranged by size: 4273 752 421 83 EarI CTCTTCn'nnn_ Cuts at: 0 641 687 1424 3302 4115 4551 5070 5529 Size: 641 46 737 1878 813 436 519 459 Fragments arranged by size: 1878 813 737 641 519 459 436 46 EcoICRI GAG'CTC Cuts at: 0 2226 3056 5529 Size: 2226 830 2473 Fragments arranged by size: 2473 2226 830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 665 695 1546 1901 3226 3241 3304 Size: 49 616 30 851 355 1325 15 63 Cuts at: 3304 3497 3571 3803 4263 4279 4313 4542 4867 Size: 193 74 232 460 16 34 229 325 Cuts at: 4867 4927 5219 5529 Size: 60 292 310 Fragments arranged by size: 1325 851 616 460 355 325 310 292 232 229 193 74 63 60 49 34 30 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 5237 5529 Size: 5237 292 EcoRI G'AATT_C Cuts at: 0 431 2085 5529 Size: 431 1654 3444 Fragments arranged by size: 3444 1654 431 EcoRV GAT'ATC Cuts at: 0 1307 5529 Size: 1307 4222 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1186 1627 2224 4309 5047 5529 Size: 1186 441 597 2085 738 482 Fragments arranged by size: 2085 1186 738 597 482 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 892 924 1357 1389 1421 2361 2393 3433 Size: 892 32 433 32 32 940 32 1040 Cuts at: 3433 3459 3465 3491 3508 3540 3889 3921 4579 Size: 26 6 26 17 32 349 32 658 Cuts at: 4579 4611 4804 4836 4902 4934 5169 5201 5529 Size: 32 193 32 66 32 235 32 328 Fragments arranged by size: 1040 940 892 658 433 349 328 235 193 66 32 32 32 32 32 32 32 32 32 32 26 26 17 6 HincII GTy'rAC Cuts at: 0 736 1370 3766 4542 4915 5529 Size: 736 634 2396 776 373 614 Fragments arranged by size: 2396 776 736 634 614 373 HindIII A'AGCT_T Cuts at: 0 970 3316 4990 5529 Size: 970 2346 1674 539 Fragments arranged by size: 2346 1674 970 539 MfeI C'AATT_G Cuts at: 0 286 2497 2974 3892 5529 Size: 286 2211 477 918 1637 Fragments arranged by size: 2211 1637 918 477 286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 803 1067 2547 3614 5529 Size: 803 264 1480 1067 1915 Fragments arranged by size: 1915 1480 1067 803 264 MscI TGG'CCA Cuts at: 0 4358 5110 5529 Size: 4358 752 419 Fragments arranged by size: 4358 752 419 MslI CAynn'nnrTG Cuts at: 0 244 2155 4781 4808 5529 Size: 244 1911 2626 27 721 Fragments arranged by size: 2626 1911 721 244 27 MspA1I CmG'CkG Cuts at: 0 463 1716 2436 5434 5529 Size: 463 1253 720 2998 95 Fragments arranged by size: 2998 1253 720 463 95 NcoI C'CATG_G Cuts at: 0 4409 5529 Size: 4409 1120 PasI CC'CwG_GG Cuts at: 0 1347 5134 5529 Size: 1347 3787 395 Fragments arranged by size: 3787 1347 395 PflMI CCAn_nnn'nTGG Cuts at: 0 3070 3894 4365 5529 Size: 3070 824 471 1164 Fragments arranged by size: 3070 1164 824 471 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1098 1130 5529 Size: 1098 32 4399 Fragments arranged by size: 4399 1098 32 PshAI GACnn'nnGTC Cuts at: 0 4610 5529 Size: 4610 919 PsiI TTA'TAA Cuts at: 0 1025 1859 2966 5529 Size: 1025 834 1107 2563 Fragments arranged by size: 2563 1107 1025 834 PstI C_TGCA'G Cuts at: 0 3714 4554 5460 5529 Size: 3714 840 906 69 Fragments arranged by size: 3714 906 840 69 PvuII CAG'CTG Cuts at: 0 2436 5434 5529 Size: 2436 2998 95 Fragments arranged by size: 2998 2436 95 SacI G_AGCT'C Cuts at: 0 2228 3058 5529 Size: 2228 830 2471 Fragments arranged by size: 2471 2228 830 SalI G'TCGA_C Cuts at: 0 734 4913 5529 Size: 734 4179 616 Fragments arranged by size: 4179 734 616 SapI GCTCTTCn'nnn_ Cuts at: 0 641 1424 5529 Size: 641 783 4105 Fragments arranged by size: 4105 783 641 ScaI AGT'ACT Cuts at: 0 2844 4440 4849 5529 Size: 2844 1596 409 680 Fragments arranged by size: 2844 1596 680 409 SexAI A'CCwGG_T Cuts at: 0 146 4397 5181 5529 Size: 146 4251 784 348 Fragments arranged by size: 4251 784 348 146 SfcI C'TryA_G Cuts at: 0 1454 2167 3710 3759 4006 4517 4550 5456 Size: 1454 713 1543 49 247 511 33 906 Cuts at: 5456 5529 Size: 73 Fragments arranged by size: 1543 1454 906 713 511 247 73 49 33 SmlI C'TyrA_G Cuts at: 0 841 1707 2209 2794 2893 3214 3571 5016 Size: 841 866 502 585 99 321 357 1445 Cuts at: 5016 5246 5529 Size: 230 283 Fragments arranged by size: 1445 866 841 585 502 357 321 283 230 99 SpeI A'CTAG_T Cuts at: 0 1315 5529 Size: 1315 4214 StuI AGG'CCT Cuts at: 0 1978 5529 Size: 1978 3551 StyI C'CwwG_G Cuts at: 0 4364 4409 5393 5529 Size: 4364 45 984 136 Fragments arranged by size: 4364 984 136 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4278 5529 Size: 4278 1251 TatI w'GTAC_w Cuts at: 0 2842 4073 4438 4847 5001 5478 5529 Size: 2842 1231 365 409 154 477 51 Fragments arranged by size: 2842 1231 477 409 365 154 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 220 252 5529 Size: 220 32 5277 Fragments arranged by size: 5277 220 32 Tth111I GACn'n_nGTC Cuts at: 0 3270 5183 5529 Size: 3270 1913 346 Fragments arranged by size: 3270 1913 346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 4395 5529 Size: 4395 1134 XmnI GAAnn'nnTTC Cuts at: 0 431 1433 3494 4289 5529 Size: 431 1002 2061 795 1240 Fragments arranged by size: 2061 1240 1002 795 431 ZraI GAC'GTC Cuts at: 0 4102 5529 Size: 4102 1427 Enzymes that do cut: AarI AatII AccI AclI AcuI AflII AleI AloI AlwNI ApoI AseI BanII BbsI BclI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI PasI PflMI PpiI PshAI PsiI PstI PvuII SacI SalI SapI ScaI SexAI SfcI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: Acc65I AfeI AflIII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI EagI EciI EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI XbaI XhoI XmaI