(Circular) (Six-base) MAPSORT of: pFN21AE2527.seq Check: 7606 from: 1 to: 10315 pFN21AE2527 10315 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6818 6818 Size: 10315 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6106 8125 278 Size: 53 83 186 936 4570 2019 2468 Fragments arranged by size: 4570 2468 2019 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2740 7000 7489 7658 1937 Size: 803 4260 489 169 4594 Fragments arranged by size: 4594 4260 803 489 169 Acc65I G'GTAC_C Cuts at: 7636 7636 Size: 10315 AclI AA'CG_TT Cuts at: 1563 2075 4712 8442 8815 1563 Size: 512 2637 3730 373 3063 Fragments arranged by size: 3730 3063 2637 512 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 3551 3906 5228 5243 5306 5499 5573 Size: 1038 881 355 1322 15 63 193 74 Cuts at: 5573 5805 6281 6315 6628 7305 8378 9762 10030 Size: 232 476 34 313 677 1073 1384 268 Cuts at: 10030 1632 Size: 1917 Fragments arranged by size: 1917 1384 1322 1073 1038 881 677 476 355 313 268 232 193 74 63 34 15 AflII C'TTAA_G Cuts at: 820 1017 3712 4214 4895 820 Size: 197 2695 502 681 6240 Fragments arranged by size: 6240 2695 681 502 197 AflIII A'CryG_T Cuts at: 1203 9214 10191 1203 Size: 8011 977 1327 Fragments arranged by size: 8011 1327 977 AgeI A'CCGG_T Cuts at: 9146 9146 Size: 10315 AhdI GACnn_n'nnGTC Cuts at: 9044 9044 Size: 10315 AleI CACnn'nnGTG Cuts at: 1166 6867 1166 Size: 5701 4614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10315 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6723 6755 6723 Size: 32 10283 AlwNI CAG_nnn'CTG Cuts at: 5711 7523 8058 9630 5711 Size: 1812 535 1572 6396 Fragments arranged by size: 6396 1812 1572 535 ApaLI G'TGCA_C Cuts at: 8372 9528 8372 Size: 1156 9159 ApoI r'AATT_y Cuts at: 1493 2016 2264 2423 2436 2893 3023 3580 3646 Size: 523 248 159 13 457 130 557 66 Cuts at: 3646 3774 3824 3997 4090 4585 5332 5809 5864 Size: 128 50 173 93 495 747 477 55 Cuts at: 5864 5956 5991 6146 6532 6875 7579 7624 7850 Size: 92 35 155 386 343 704 45 226 Cuts at: 7850 8002 10111 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 747 704 557 523 495 477 457 386 343 248 226 173 159 155 152 130 128 93 92 66 55 50 45 35 13 AseI AT'TA_AT Cuts at: 160 1032 2169 3227 5144 6002 6231 8869 160 Size: 872 1137 1058 1917 858 229 2638 1606 Fragments arranged by size: 2638 1917 1606 1137 1058 872 858 229 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10315 AvaI C'yCGr_G Cuts at: 1870 1943 7640 1870 Size: 73 5697 4545 Fragments arranged by size: 5697 4545 73 BamHI G'GATC_C Cuts at: 7645 8012 7645 Size: 367 9948 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7636 9091 618 Size: 325 217 627 5849 1455 1842 Fragments arranged by size: 5849 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4233 5060 7634 1391 Size: 2842 827 2574 4072 Fragments arranged by size: 4072 2842 2574 827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 3102 3557 3699 4760 5085 5391 Size: 971 1164 39 455 142 1061 325 306 Cuts at: 5391 5448 5604 5813 6696 928 Size: 57 156 209 883 4547 Fragments arranged by size: 4547 1164 1061 971 883 455 325 306 209 156 142 57 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8506 8540 1903 Size: 34 6569 34 3678 Fragments arranged by size: 6569 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8207 9417 8207 Size: 1210 9105 BclI T'GATC_A Cuts at: 1548 2295 5469 1548 Size: 747 3174 6394 Fragments arranged by size: 6394 3174 747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8926 136 Size: 107 122 71 631 605 7254 1525 Fragments arranged by size: 7254 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 10310 10310 Size: 10315 BlpI GC'TnA_GC Cuts at: 3612 3812 4167 5939 7732 3612 Size: 200 355 1772 1793 6195 Fragments arranged by size: 6195 1793 1772 355 200 Bme1580I G_kGCm'C Cuts at: 1790 8376 9532 1790 Size: 6586 1156 2573 Fragments arranged by size: 6586 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6956 8046 9004 Size: 416 53 83 399 24 5539 1090 958 Cuts at: 9004 442 Size: 1753 Fragments arranged by size: 5539 1753 1090 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10315 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5079 5079 Size: 10315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2700 6265 6953 7013 8975 1239 Size: 815 646 3565 688 60 1962 2579 Fragments arranged by size: 3565 2579 1962 815 688 646 60 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 10315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 4820 5237 5594 7087 7317 8440 9305 9603 Size: 1953 417 357 1493 230 1123 865 298 Cuts at: 9603 2867 Size: 3579 Fragments arranged by size: 3579 1953 1493 1123 865 417 357 298 230 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 5583 7258 8978 882 Size: 617 1170 2914 1675 1720 2219 Fragments arranged by size: 2914 2219 1720 1675 1170 617 BsaAI yAC'GTr Cuts at: 493 1204 4727 8106 493 Size: 711 3523 3379 2702 Fragments arranged by size: 3523 3379 2702 711 BsaBI GATnn'nnATC Cuts at: 6231 10309 6231 Size: 4078 6237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6103 8122 8504 9139 Size: 53 83 186 936 4570 2019 382 635 Cuts at: 9139 275 Size: 1451 Fragments arranged by size: 4570 2019 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8748 9146 9420 9567 9978 8748 Size: 398 274 147 411 9085 Fragments arranged by size: 9085 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 10090 Fragments arranged by size: 10090 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5052 6153 1186 Size: 3866 1101 5348 Fragments arranged by size: 5348 3866 1101 BseYI C'CCAG_C Cuts at: 3294 9518 10014 3294 Size: 6224 496 3595 Fragments arranged by size: 6224 3595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2418 2679 3756 6509 6852 1163 Size: 573 682 261 1077 2753 343 4626 Fragments arranged by size: 4626 2753 1077 682 573 343 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7765 8526 9554 1540 Size: 275 186 5764 761 1028 2301 Fragments arranged by size: 5764 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3121 4233 5060 6969 8376 8461 9532 3121 Size: 1112 827 1909 1407 85 1071 3904 Fragments arranged by size: 3904 1909 1407 1112 1071 827 85 BsmI GAATG_Cn' Cuts at: 2235 5317 5380 7826 7919 2235 Size: 3082 63 2446 93 4631 Fragments arranged by size: 4631 3082 2446 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3121 4233 5060 6969 7634 8376 8461 Size: 399 1331 1112 827 1909 665 742 85 Cuts at: 8461 9532 1391 Size: 1071 2174 Fragments arranged by size: 2174 1909 1331 1112 1071 827 742 665 399 85 BspEI T'CCGG_A Cuts at: 9978 9978 Size: 10315 BspHI T'CATG_A Cuts at: 2043 2298 3178 3781 4792 5364 5479 5500 5698 Size: 255 880 603 1011 572 115 21 198 Cuts at: 5698 6889 8204 9934 2043 Size: 1191 1315 1730 2424 Fragments arranged by size: 2424 1730 1315 1191 1011 880 603 572 255 198 115 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 6818 7581 7670 844 Size: 1685 4289 763 89 3489 Fragments arranged by size: 4289 3489 1685 763 89 BsrBI CCG'CTC Cuts at: 1394 1823 2656 6273 8202 1394 Size: 429 833 3617 1929 3507 Fragments arranged by size: 3617 3507 1929 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2531 2585 3475 4388 8810 8984 65 Size: 1183 1283 54 890 913 4422 174 1396 Fragments arranged by size: 4422 1396 1283 1183 913 890 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 8959 9146 1700 Size: 110 7149 187 2869 Fragments arranged by size: 7149 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10315 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10315 BssSI C'ACGA_G Cuts at: 3114 7410 8375 9387 3114 Size: 4296 965 1012 4042 Fragments arranged by size: 4296 4042 1012 965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2471 794 Size: 1677 8638 BstBI TT'CG_AA Cuts at: 9119 9119 Size: 10315 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10315 BstXI CCAn_nnnn'nTGG Cuts at: 2056 6658 6869 2056 Size: 4602 211 5502 Fragments arranged by size: 5502 4602 211 BstYI r'GATC_y Cuts at: 1972 3592 6049 6647 7645 8012 8397 8414 9866 Size: 1620 2457 598 998 367 385 17 1452 Cuts at: 9866 9952 9964 10310 1972 Size: 86 12 346 1977 Fragments arranged by size: 2457 1977 1620 1452 998 598 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 6495 513 Size: 552 939 4491 4333 Fragments arranged by size: 4491 4333 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9576 Fragments arranged by size: 9576 683 56 BtsI GCAGTG_nn' Cuts at: 5171 6874 6919 7539 7831 8625 8645 5171 Size: 1703 45 620 292 794 20 6841 Fragments arranged by size: 6841 1703 794 620 292 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10280 DraI TTT'AAA Cuts at: 3957 4051 5775 7619 3957 Size: 94 1724 1844 6653 Fragments arranged by size: 6653 1844 1724 94 DraIII CAC_nnn'GTG Cuts at: 2708 4727 6707 2708 Size: 2019 1980 6316 Fragments arranged by size: 6316 2019 1980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2545 9322 809 Size: 507 1229 6777 1802 Fragments arranged by size: 6777 1802 1229 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 6358 7194 Size: 54 1425 50 275 18 258 4270 836 Cuts at: 7194 7762 8651 10280 8 Size: 568 889 1629 43 Fragments arranged by size: 4270 1629 1425 889 836 568 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7762 1537 Size: 275 5950 4090 Fragments arranged by size: 5950 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 2692 3429 5304 6117 6637 7156 8245 Size: 1293 46 737 1875 813 520 519 1089 Cuts at: 8245 1353 Size: 3423 Fragments arranged by size: 3423 1875 1293 1089 813 737 520 519 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8902 9276 9422 387 Size: 1064 138 7313 374 146 1280 Fragments arranged by size: 7313 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4231 5058 4231 Size: 827 9488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2670 2700 3551 3906 5228 5243 Size: 393 422 616 30 851 355 1322 15 Cuts at: 5243 5306 5499 5573 5805 6265 6281 6315 6628 Size: 63 193 74 232 460 16 34 313 Cuts at: 6628 6953 7013 7305 8378 8975 9762 10030 1239 Size: 325 60 292 1073 597 787 268 1524 Fragments arranged by size: 1524 1322 1073 851 787 616 597 460 422 393 355 325 313 292 268 232 193 74 63 60 34 30 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 6415 7323 6415 Size: 908 9407 EcoRI G'AATT_C Cuts at: 2436 4090 7624 8002 2436 Size: 1654 3534 378 4749 Fragments arranged by size: 4749 3534 1654 378 EcoRV GAT'ATC Cuts at: 3312 3312 Size: 10315 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3191 3632 4229 6311 7133 3191 Size: 441 597 2082 822 6373 Fragments arranged by size: 6373 2082 822 597 441 FspI TGC'GCA Cuts at: 8821 8821 Size: 10315 HaeII r_GCGC'y Cuts at: 1678 9462 1678 Size: 7784 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 3362 3394 3426 Size: 32 135 32 1325 32 433 32 32 Cuts at: 3426 4366 4398 5435 5461 5467 5493 5510 5542 Size: 940 32 1037 26 6 26 17 32 Cuts at: 5542 5891 5923 6665 6697 6890 6922 6988 7020 Size: 349 32 742 32 193 32 66 32 Cuts at: 7020 7255 7287 8956 8988 9030 9062 1373 Size: 235 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 1325 1037 940 742 433 349 235 193 135 66 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 26 26 17 6 HincII GTy'rAC Cuts at: 669 1657 1938 2741 3375 5768 6628 7001 7659 Size: 988 281 803 634 2393 860 373 658 Cuts at: 7659 7905 669 Size: 246 3079 Fragments arranged by size: 3079 2393 988 860 803 658 634 373 281 246 HindIII A'AGCT_T Cuts at: 748 2975 5318 7076 748 Size: 2227 2343 1758 3987 Fragments arranged by size: 3987 2343 2227 1758 HpaI GTT'AAC Cuts at: 7905 7905 Size: 10315 KpnI G_GTAC'C Cuts at: 7640 7640 Size: 10315 MfeI C'AATT_G Cuts at: 2291 4502 4976 5894 7914 2291 Size: 2211 474 918 2020 4692 Fragments arranged by size: 4692 2211 2020 918 474 MluI A'CGCG_T Cuts at: 10191 10191 Size: 10315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 3072 4552 5616 9429 9613 1305 Size: 1503 264 1480 1064 3813 184 2007 Fragments arranged by size: 3813 2007 1503 1480 1064 264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6360 7196 10 Size: 54 1425 343 4528 836 3129 Fragments arranged by size: 4528 3129 1425 836 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 4160 6473 6867 6894 8273 8632 Size: 648 1083 1911 2313 394 27 1379 359 Cuts at: 8632 8791 518 Size: 159 2042 Fragments arranged by size: 2313 2042 1911 1379 1083 648 394 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2468 3721 4441 7520 7731 8408 9556 9801 Size: 862 1253 720 3079 211 677 1148 245 Cuts at: 9801 1606 Size: 2120 Fragments arranged by size: 3079 2120 1253 1148 862 720 677 245 211 NcoI C'CATG_G Cuts at: 513 1065 2004 6495 513 Size: 552 939 4491 4333 Fragments arranged by size: 4491 4333 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10315 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10315 NotI GC'GGCC_GC Cuts at: 7762 7762 Size: 10315 NspI r_CATG'y Cuts at: 1132 1229 7673 9218 1132 Size: 97 6444 1545 2229 Fragments arranged by size: 6444 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3352 7220 1348 Size: 2004 3868 4443 Fragments arranged by size: 4443 3868 2004 PciI A'CATG_T Cuts at: 9214 9214 Size: 10315 PflMI CCAn_nnn'nTGG Cuts at: 1108 5072 5896 6367 1108 Size: 3964 824 471 5056 Fragments arranged by size: 5056 3964 824 471 PmeI GTTT'AAAC Cuts at: 7619 7619 Size: 10315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3103 3135 8384 8416 9897 9929 3103 Size: 32 5249 32 1481 32 3489 Fragments arranged by size: 5249 3489 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 6696 1613 Size: 5083 5232 PsiI TTA'TAA Cuts at: 3030 3864 4968 7885 3030 Size: 834 1104 2917 5460 Fragments arranged by size: 5460 2917 1104 834 PstI C_TGCA'G Cuts at: 830 5716 6640 7546 7667 830 Size: 4886 924 906 121 3478 Fragments arranged by size: 4886 3478 924 906 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10315 PvuII CAG'CTG Cuts at: 4441 7520 4441 Size: 3079 7236 SacI G_AGCT'C Cuts at: 4233 5060 4233 Size: 827 9488 SalI G'TCGA_C Cuts at: 1936 2739 6999 7657 1936 Size: 803 4260 658 4594 Fragments arranged by size: 4594 4260 803 658 SapI GCTCTTCn'nnn_ Cuts at: 2646 3429 2646 Size: 783 9532 SbfI CC_TGCA'GG Cuts at: 7667 7667 Size: 10315 ScaI AGT'ACT Cuts at: 4849 6526 6935 8563 10275 4849 Size: 1677 409 1628 1712 4889 Fragments arranged by size: 4889 1712 1677 1628 409 SexAI A'CCwGG_T Cuts at: 2151 6483 7267 2151 Size: 4332 784 5199 Fragments arranged by size: 5199 4332 784 SfcI C'TryA_G Cuts at: 826 1045 3459 4172 5712 5761 6008 6603 6636 Size: 219 2414 713 1540 49 247 595 33 Cuts at: 6636 7542 7663 8798 9479 9670 826 Size: 906 121 1135 681 191 1471 Fragments arranged by size: 2414 1540 1471 1135 906 713 681 595 247 219 191 121 49 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10315 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10315 SmaI CCC'GGG Cuts at: 1872 7642 1872 Size: 5770 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 3712 4214 4799 4895 5216 Size: 197 926 903 866 502 585 96 321 Cuts at: 5216 5573 7102 7332 8419 9320 9582 820 Size: 357 1529 230 1087 901 262 1553 Fragments arranged by size: 1553 1529 1087 926 903 901 866 585 502 357 321 262 230 197 96 SnaBI TAC'GTA Cuts at: 493 8106 493 Size: 7613 2702 SpeI A'CTAG_T Cuts at: 152 3320 152 Size: 3168 7147 SphI G_CATG'C Cuts at: 1132 7673 1132 Size: 6541 3774 SspI AAT'ATT Cuts at: 5 52 8239 5 Size: 47 8187 2081 Fragments arranged by size: 8187 2081 47 StuI AGG'CCT Cuts at: 1517 3983 6452 1517 Size: 2466 2469 5380 Fragments arranged by size: 5380 2469 2466 StyI C'CwwG_G Cuts at: 513 1065 2004 6366 6495 7479 7754 513 Size: 552 939 4362 129 984 275 3074 Fragments arranged by size: 4362 3074 984 939 552 275 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6280 6442 8342 8359 8512 8697 9142 Size: 1255 4700 162 1900 17 153 185 445 Cuts at: 9142 325 Size: 1498 Fragments arranged by size: 4700 1900 1498 1255 445 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4847 6075 6524 Size: 274 80 33 51 1443 2870 1228 449 Cuts at: 6524 6933 7087 7564 8561 10273 96 Size: 409 154 477 997 1712 138 Fragments arranged by size: 2870 1712 1443 1228 997 477 449 409 274 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 10275 10307 2225 Size: 32 8018 32 2233 Fragments arranged by size: 8018 2233 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5272 7269 1316 Size: 3956 1997 4362 Fragments arranged by size: 4362 3956 1997 XbaI T'CTAG_A Cuts at: 7651 7651 Size: 10315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6481 6481 Size: 10315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10315 XmaI C'CCGG_G Cuts at: 1870 7640 1870 Size: 5770 4545 XmnI GAAnn'nnTTC Cuts at: 2436 3438 5496 6291 8444 2436 Size: 1002 2058 795 2153 4307 Fragments arranged by size: 4307 2153 2058 1002 795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6104 8123 276 Size: 53 83 186 936 4570 2019 2468 Fragments arranged by size: 4570 2468 2019 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI