Restriction map for
pFN21AE2527
(Linear) (Six-base) MAPSORT of: KIEE2527.seq Check: 9834 from: 1 to: 5610
KIEE2527.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4813 5610
Size: 4813 797
AatII G_ACGT'C
Cuts at: 0 4101 5610
Size: 4101 1509
AccI GT'mk_AC
Cuts at: 0 735 4995 5484 5610
Size: 735 4260 489 126
Fragments arranged by size:
4260 735 489 126
AclI AA'CG_TT
Cuts at: 0 70 2707 5610
Size: 70 2637 2903
Fragments arranged by size:
2903 2637 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1546 1901 3223 3238 3301 3494 3568
Size: 665 881 355 1322 15 63 193 74
Cuts at: 3568 3800 4276 4310 4623 5300 5610
Size: 232 476 34 313 677 310
Fragments arranged by size:
1322 881 677 665 476 355 313 310
232 193 74 63 34 15
AflII C'TTAA_G
Cuts at: 0 1707 2209 2890 5610
Size: 1707 502 681 2720
Fragments arranged by size:
2720 1707 681 502
AleI CACnn'nnGTG
Cuts at: 0 4862 5610
Size: 4862 748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4718 4750 5610
Size: 4718 32 860
Fragments arranged by size:
4718 860 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3706 5518 5610
Size: 3706 1812 92
Fragments arranged by size:
3706 1812 92
ApoI r'AATT_y
Cuts at: 0 11 259 418 431 888 1018 1575 1641
Size: 11 248 159 13 457 130 557 66
Cuts at: 1641 1769 1819 1992 2085 2580 3327 3804 3859
Size: 128 50 173 93 495 747 477 55
Cuts at: 3859 3951 3986 4141 4527 4870 5574 5610
Size: 92 35 155 386 343 704 36
Fragments arranged by size:
747 704 557 495 477 457 386 343
248 173 159 155 130 128 93 92
66 55 50 36 35 13 11
AseI AT'TA_AT
Cuts at: 0 164 1222 3139 3997 4226 5610
Size: 164 1058 1917 858 229 1384
Fragments arranged by size:
1917 1384 1058 858 229 164
BanII G_rGCy'C
Cuts at: 0 2228 3055 5610
Size: 2228 827 2555
Fragments arranged by size:
2555 2228 827
BbsI GAAGACnn'nnnn_
Cuts at: 0 1058 1097 1552 1694 2755 3080 3386 3443
Size: 1058 39 455 142 1061 325 306 57
Cuts at: 3443 3599 3808 4691 5610
Size: 156 209 883 919
Fragments arranged by size:
1061 1058 919 883 455 325 306 209
156 142 57 39
BclI T'GATC_A
Cuts at: 0 290 3464 5610
Size: 290 3174 2146
Fragments arranged by size:
3174 2146 290
BlpI GC'TnA_GC
Cuts at: 0 1607 1807 2162 3934 5610
Size: 1607 200 355 1772 1676
Fragments arranged by size:
1772 1676 1607 355 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 4951 5610
Size: 4951 659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3074 5610
Size: 3074 2536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 695 4260 4948 5008 5610
Size: 49 646 3565 688 60 602
Fragments arranged by size:
3565 688 646 602 60 49
Bpu10I CC'TnA_GC
Cuts at: 0 787 5610
Size: 787 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2815 3232 3589 5082 5312 5610
Size: 862 1953 417 357 1493 230 298
Fragments arranged by size:
1953 1493 862 417 357 298 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 3578 5253 5610
Size: 664 2914 1675 357
Fragments arranged by size:
2914 1675 664 357
BsaAI yAC'GTr
Cuts at: 0 2722 5610
Size: 2722 2888
BsaBI GATnn'nnATC
Cuts at: 0 4226 5610
Size: 4226 1384
BsaHI Gr'CG_yC
Cuts at: 0 4098 5610
Size: 4098 1512
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3047 4148 5610
Size: 3047 1101 1462
Fragments arranged by size:
3047 1462 1101
BseYI C'CCAG_C
Cuts at: 0 1289 5610
Size: 1289 4321
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 674 1751 4504 4847 5610
Size: 413 261 1077 2753 343 763
Fragments arranged by size:
2753 1077 763 413 343 261
BsiHKAI G_wGCw'C
Cuts at: 0 1116 2228 3055 4964 5610
Size: 1116 1112 827 1909 646
Fragments arranged by size:
1909 1116 1112 827 646
BsmI GAATG_Cn'
Cuts at: 0 230 3312 3375 5610
Size: 230 3082 63 2235
Fragments arranged by size:
3082 2235 230 63
Bsp1286I G_dGCh'C
Cuts at: 0 1116 2228 3055 4964 5610
Size: 1116 1112 827 1909 646
Fragments arranged by size:
1909 1116 1112 827 646
BspHI T'CATG_A
Cuts at: 0 38 293 1173 1776 2787 3359 3474 3495
Size: 38 255 880 603 1011 572 115 21
Cuts at: 3495 3693 4884 5610
Size: 198 1191 726
Fragments arranged by size:
1191 1011 880 726 603 572 255 198
115 38 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 4813 5576 5610
Size: 524 4289 763 34
Fragments arranged by size:
4289 763 524 34
BsrBI CCG'CTC
Cuts at: 0 651 4268 5610
Size: 651 3617 1342
Fragments arranged by size:
3617 1342 651
BsrDI GCAATG_nn'
Cuts at: 0 526 580 1470 2383 5610
Size: 526 54 890 913 3227
Fragments arranged by size:
3227 913 890 526 54
BssSI C'ACGA_G
Cuts at: 0 1109 5405 5610
Size: 1109 4296 205
Fragments arranged by size:
4296 1109 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 466 5610
Size: 466 5144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 4653 4864 5610
Size: 51 4602 211 746
Fragments arranged by size:
4602 746 211 51
BstYI r'GATC_y
Cuts at: 0 1587 4044 4642 5610
Size: 1587 2457 598 968
Fragments arranged by size:
2457 1587 968 598
BtgI C'CryG_G
Cuts at: 0 4490 5610
Size: 4490 1120
BtsI GCAGTG_nn'
Cuts at: 0 3166 4869 4914 5534 5610
Size: 3166 1703 45 620 76
Fragments arranged by size:
3166 1703 620 76 45
DraI TTT'AAA
Cuts at: 0 1952 2046 3770 5610
Size: 1952 94 1724 1840
Fragments arranged by size:
1952 1840 1724 94
DraIII CAC_nnn'GTG
Cuts at: 0 703 2722 4702 5610
Size: 703 2019 1980 908
Fragments arranged by size:
2019 1980 908 703
DrdI GACnn_nn'nnGTC
Cuts at: 0 540 5610
Size: 540 5070
EaeI y'GGCC_r
Cuts at: 0 83 4353 5189 5610
Size: 83 4270 836 421
Fragments arranged by size:
4270 836 421 83
EarI CTCTTCn'nnn_
Cuts at: 0 641 687 1424 3299 4112 4632 5151 5610
Size: 641 46 737 1875 813 520 519 459
Fragments arranged by size:
1875 813 737 641 520 519 459 46
EcoICRI GAG'CTC
Cuts at: 0 2226 3053 5610
Size: 2226 827 2557
Fragments arranged by size:
2557 2226 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 665 695 1546 1901 3223 3238 3301
Size: 49 616 30 851 355 1322 15 63
Cuts at: 3301 3494 3568 3800 4260 4276 4310 4623 4948
Size: 193 74 232 460 16 34 313 325
Cuts at: 4948 5008 5300 5610
Size: 60 292 310
Fragments arranged by size:
1322 851 616 460 355 325 313 310
292 232 193 74 63 60 49 34
30 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4410 5318 5610
Size: 4410 908 292
Fragments arranged by size:
4410 908 292
EcoRI G'AATT_C
Cuts at: 0 431 2085 5610
Size: 431 1654 3525
Fragments arranged by size:
3525 1654 431
EcoRV GAT'ATC
Cuts at: 0 1307 5610
Size: 1307 4303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1186 1627 2224 4306 5128 5610
Size: 1186 441 597 2082 822 482
Fragments arranged by size:
2082 1186 822 597 482 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1357 1389 1421 2361 2393 3430
Size: 892 32 433 32 32 940 32 1037
Cuts at: 3430 3456 3462 3488 3505 3537 3886 3918 4660
Size: 26 6 26 17 32 349 32 742
Cuts at: 4660 4692 4885 4917 4983 5015 5250 5282 5610
Size: 32 193 32 66 32 235 32 328
Fragments arranged by size:
1037 940 892 742 433 349 328 235
193 66 32 32 32 32 32 32
32 32 32 32 26 26 17 6
HincII GTy'rAC
Cuts at: 0 736 1370 3763 4623 4996 5610
Size: 736 634 2393 860 373 614
Fragments arranged by size:
2393 860 736 634 614 373
HindIII A'AGCT_T
Cuts at: 0 970 3313 5071 5610
Size: 970 2343 1758 539
Fragments arranged by size:
2343 1758 970 539
MfeI C'AATT_G
Cuts at: 0 286 2497 2971 3889 5610
Size: 286 2211 474 918 1721
Fragments arranged by size:
2211 1721 918 474 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1067 2547 3611 5610
Size: 803 264 1480 1064 1999
Fragments arranged by size:
1999 1480 1064 803 264
MscI TGG'CCA
Cuts at: 0 4355 5191 5610
Size: 4355 836 419
Fragments arranged by size:
4355 836 419
MslI CAynn'nnrTG
Cuts at: 0 244 2155 4468 4862 4889 5610
Size: 244 1911 2313 394 27 721
Fragments arranged by size:
2313 1911 721 394 244 27
MspA1I CmG'CkG
Cuts at: 0 463 1716 2436 5515 5610
Size: 463 1253 720 3079 95
Fragments arranged by size:
3079 1253 720 463 95
NcoI C'CATG_G
Cuts at: 0 4490 5610
Size: 4490 1120
PasI CC'CwG_GG
Cuts at: 0 1347 5215 5610
Size: 1347 3868 395
Fragments arranged by size:
3868 1347 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 3067 3891 4362 5610
Size: 3067 824 471 1248
Fragments arranged by size:
3067 1248 824 471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1130 5610
Size: 1098 32 4480
Fragments arranged by size:
4480 1098 32
PshAI GACnn'nnGTC
Cuts at: 0 4691 5610
Size: 4691 919
PsiI TTA'TAA
Cuts at: 0 1025 1859 2963 5610
Size: 1025 834 1104 2647
Fragments arranged by size:
2647 1104 1025 834
PstI C_TGCA'G
Cuts at: 0 3711 4635 5541 5610
Size: 3711 924 906 69
Fragments arranged by size:
3711 924 906 69
PvuII CAG'CTG
Cuts at: 0 2436 5515 5610
Size: 2436 3079 95
Fragments arranged by size:
3079 2436 95
SacI G_AGCT'C
Cuts at: 0 2228 3055 5610
Size: 2228 827 2555
Fragments arranged by size:
2555 2228 827
SalI G'TCGA_C
Cuts at: 0 734 4994 5610
Size: 734 4260 616
Fragments arranged by size:
4260 734 616
SapI GCTCTTCn'nnn_
Cuts at: 0 641 1424 5610
Size: 641 783 4186
Fragments arranged by size:
4186 783 641
ScaI AGT'ACT
Cuts at: 0 2844 4521 4930 5610
Size: 2844 1677 409 680
Fragments arranged by size:
2844 1677 680 409
SexAI A'CCwGG_T
Cuts at: 0 146 4478 5262 5610
Size: 146 4332 784 348
Fragments arranged by size:
4332 784 348 146
SfcI C'TryA_G
Cuts at: 0 1454 2167 3707 3756 4003 4598 4631 5537
Size: 1454 713 1540 49 247 595 33 906
Cuts at: 5537 5610
Size: 73
Fragments arranged by size:
1540 1454 906 713 595 247 73 49
33
SmlI C'TyrA_G
Cuts at: 0 841 1707 2209 2794 2890 3211 3568 5097
Size: 841 866 502 585 96 321 357 1529
Cuts at: 5097 5327 5610
Size: 230 283
Fragments arranged by size:
1529 866 841 585 502 357 321 283
230 96
SpeI A'CTAG_T
Cuts at: 0 1315 5610
Size: 1315 4295
StuI AGG'CCT
Cuts at: 0 1978 4447 5610
Size: 1978 2469 1163
Fragments arranged by size:
2469 1978 1163
StyI C'CwwG_G
Cuts at: 0 4361 4490 5474 5610
Size: 4361 129 984 136
Fragments arranged by size:
4361 984 136 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4275 4437 5610
Size: 4275 162 1173
Fragments arranged by size:
4275 1173 162
TatI w'GTAC_w
Cuts at: 0 2842 4070 4519 4928 5082 5559 5610
Size: 2842 1228 449 409 154 477 51
Fragments arranged by size:
2842 1228 477 449 409 154 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 5610
Size: 220 32 5358
Fragments arranged by size:
5358 220 32
Tth111I GACn'n_nGTC
Cuts at: 0 3267 5264 5610
Size: 3267 1997 346
Fragments arranged by size:
3267 1997 346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4476 5610
Size: 4476 1134
XmnI GAAnn'nnTTC
Cuts at: 0 431 1433 3491 4286 5610
Size: 431 1002 2058 795 1324
Fragments arranged by size:
2058 1324 1002 795 431
ZraI GAC'GTC
Cuts at: 0 4099 5610
Size: 4099 1511
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AleI AloI
AlwNI ApoI AseI BanII BbsI BclI BlpI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI PasI PflMI PpiI PshAI
PsiI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SmlI SpeI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflIII AgeI AhdI AlfI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmtI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI EagI EciI EcoO109I FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI XbaI XhoI XmaI