################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlpI Bpu1102I BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RsrII SacII SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1897 GTCGAC GT^[AC][GT]AC AgeI 1607 ACCGGT A^CCGGT AlwNI 794 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 926 CCTCAGG CC^T[ACGT]AGG AspI 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1378 CCTAGG C^CTAGG BanI 1108 GGCGCC G^G[CT][GA]CC BbeI 1108 GGCGCC GGCGC^C BclI 1515 TGATCA T^GATCA BlnI 1378 CCTAGG C^CTAGG BsaAI 891 CACGTA [CT]AC^GT[GA] BsmBI 1657 GAGACG CGTCTC(1/5) BstXI 1416 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1327 AGATCC [GA]^GATC[CT] Bsu36I 926 CCTCAGG CC^T[ACGT]AGG DsaI 48 CCACGG C^C[GA][CT]GG Eco81I 926 CCTCAGG CC^T[ACGT]AGG FbaI 1515 TGATCA T^GATCA HaeII 1108 GGCGCC [GA]GCGC^[CT] HpaI 1465 GTTAAC GTT^AAC KasI 1108 GGCGCC G^GCGCC MflI 1327 AGATCC [GA]^GATC[CT] MslI 514 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1353 CAGCGG C[AC]G^C[GT]G NarI 1108 GGCGCC GG^CGCC NspI 668 ACATGC [GA]CATG^[CT] PaeR7I 203 CTCGAG C^TCGAG PflFI 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1811 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1607 ACCGGT A^CCGGT SacI 695 GAGCTC GAGCT^C SalI 1897 GTCGAC G^TCGAC ScaI 227 AGTACT AGT^ACT SfoI 1108 GGCGCC GGC^GCC SmaI 209 CCCGGG CCC^GGG SmlI 203 CTCGAG C^T[CT][GA]AG SspI 320 AATATT AAT^ATT Tth111I 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1811 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 203 CTCGAG C^TCGAG XhoII 1327 AGATCC [GA]^GATC[CT] XmaCI 209 CCCGGG C^CCGGG XmaI 209 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 1108 GGCGCC G[GA]^CG[CT]C 1760 GACGCC AfaI 228 GTAC GT^AC 1493 GTAC ApaI 133 GGGCCC GGGCC^C 688 GGGCCC BbsI 2042 GAAGAC GAAGAC(2/6) 1804 GTCTTC BpuAI 2042 GAAGAC GAAGAC(2/6) 1804 GTCTTC BsaHI 1108 GGCGCC G[GA]^CG[CT]C 1760 GACGCC BsaI 157 GAGACC GGTCTC(1/5) 237 GAGACC BsaWI 563 TCCGGT [AT]^CCGG[AT] 1607 ACCGGT BseRI 409 CTCCTC GAGGAG(10/8) 412 CTCCTC BsgI 813 CTGCAC GTGCAG(16/14) 2071 GTGCAG BsmFI 973 GGGAC GGGAC(10/14) 567 GTCCC BsrBI 1152 CCGCTC CCGCTC(-3/-3) 304 GAGCGG BsrI 1073 ACTGG ACTGG(1/-1) 1423 ACTGG BstAPI 224 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1435 GCAGATGATGC EcoNI 922 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1931 CCTGTCCGAGG Hin1I 1108 GGCGCC G[GA]^CG[CT]C 1760 GACGCC HincII 1465 GTTAAC GT[CT]^[GA]AC 1897 GTCGAC HindII 1465 GTTAAC GT[CT]^[GA]AC 1897 GTCGAC PpuMI 808 AGGTCCT [GA]G^G[AT]CC[CT] 1010 AGGACCT PstI 121 CTGCAG CTGCA^G 1578 CTGCAG PvuI 184 CGATCG CGAT^CG 1661 CGATCG RsaI 228 GTAC GT^AC 1493 GTAC SapI 1077 GAAGAGC GCTCTTC(1/4) 1154 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspHI BspMI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RcaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I