Restriction map for
pFN21AE2720
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KspI MaeII
MamI MfeI MluNI MscI MunI
MwoI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 835 GGTACC G^GTACC
AccI 682 GTCTAC GT^[AC][GT]AC
AccII 250 CGCG CG^CG
AccIII 200 TCCGGA T^CCGGA
AflIII 249 ACGCGT A^C[GA][CT]GT
AocI 342 CCTGAGG CC^T[ACGT]AGG
Asp700I 565 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 835 GGTACC G^GTACC
AspHI 373 GAGCTC G[AT]GC[AT]^C
AvaII 56 GGACC G^G[AT]CC
BanII 373 GAGCTC G[GA]GC[CT]^C
BbsI 172 GAAGAC GAAGAC(2/6)
BciVI 203 GGATAC GTATCC(6/5)
BfaI 116 CTAG C^TAG
BmyI 373 GAGCTC G[AGT]GC[ACT]^C
BpuAI 172 GAAGAC GAAGAC(2/6)
BsaWI 200 TCCGGA [AT]^CCGG[AT]
BseAI 200 TCCGGA T^CCGGA
BsgI 888 CTGCAC GTGCAG(16/14)
BsiHKAI 373 GAGCTC G[AT]GC[AT]^C
BsmFI 55 GGGAC GGGAC(10/14)
Bsp1286I 373 GAGCTC G[AGT]GC[ACT]^C
BspDI 895 ATCGAT AT^CGAT
BspEI 200 TCCGGA T^CCGGA
BspMI 526 GCAGGT ACCTGC(4/8)
BstUI 250 CGCG CG^CG
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
Cac8I 231 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 744 GCGC GCG^C
ClaI 895 ATCGAT AT^CGAT
DraIII 913 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 7 CCATGG C^C[GA][CT]GG
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
FauI 537 GCGGG CCCGC(4/6)
HaeII 743 GGCGCT [GA]GCGC^[CT]
HapII 201 CCGG C^CGG
HhaI 744 GCGC GCG^C
HinP1I 744 GCGC G^CGC
HindIII 145 AAGCTT A^AGCTT
HpaII 201 CCGG C^CGG
HphI 529 GGTGA GGTGA(8/7)
KpnI 835 GGTACC GGTAC^C
MaeI 116 CTAG C^TAG
MaeIII 80 GTCAC ^GT[ACGT]AC
MluI 249 ACGCGT A^CGCGT
MroI 200 TCCGGA T^CCGGA
MslI 442 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 201 CCGG C^CGG
MvnI 250 CGCG CG^CG
NcoI 7 CCATGG C^CATGG
NdeI 701 CATATG CA^TATG
PpuMI 55 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 824 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 609 CTGCAG CTGCA^G
PvuII 880 CAGCTG CAG^CTG
SacI 373 GAGCTC GAGCT^C
SapI 259 GAAGAGC GCTCTTC(1/4)
SfaNI 812 GCATC GCATC(5/9)
SmlI 177 CTTGAG C^T[CT][GA]AG
StuI 778 AGGCCT AGG^CCT
TaqI 896 TCGA T^CGA
Tsp45I 80 GTCAC ^GT[GC]AC
TthHB8I 896 TCGA T^CGA
XcmI 311 CCAGAAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 565 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 330 GGATC GGATC(4/5)
495 GGATC
BanI 706 GGCACC G^G[CT][GA]CC
835 GGTACC
BbvI 264 GCAGC GCAGC(8/12)
611 GCAGC
BclI 108 TGATCA T^GATCA
222 TGATCA
BglII 555 AGATCT A^GATCT
805 AGATCT
BseRI 345 GAGGAG GAGGAG(10/8)
348 GAGGAG
BsmAI 766 GAGAC GTCTC(1/5)
790 GAGAC
BspHI 333 TCATGA T^CATGA
692 TCATGA
BstF5I 103 CATCC GGATG(2/0)
760 GGATG
BstXI 131 CCAAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
620 CCAAAAAGCTGG
DraII 55 GGGACCT [GA]G^G[ACGT]CC[CT]
213 AGGCCCT
EaeI 4 CGGCCA [CT]^GGCC[GA]
188 TGGCCG
EarI 242 GAAGAG CTCTTC(1/4)
259 GAAGAG
EcoO109I 55 GGGACCT [GA]G^G[ACGT]CC[CT]
213 AGGCCCT
EcoT14I 7 CCATGG C^C[AT][AT]GG
602 CCAAGG
FbaI 108 TGATCA T^GATCA
222 TGATCA
FokI 103 CATCC GGATG(9/13)
760 GGATG
Ksp632I 242 GAAGAG CTCTTC(1/4)
259 GAAGAG
MspA1I 574 CAGCGG C[AC]G^C[GT]G
880 CAGCTG
NspI 441 ACATGC [GA]CATG^[CT]
793 ACATGC
PflMI 7 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCATCATGTGG
RcaI 333 TCATGA T^CATGA
692 TCATGA
StyI 7 CCATGG C^C[AT][AT]GG
602 CCAAGG
TseI 264 GCAGC G^C[AT]GC
611 GCAGC
TspRI 819 CACTG CA[GC]TG[ACGT][ACGT]^
826 CACTG
Van91I 7 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCATCATGTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsaJI BsiYI BslI BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI ItaI
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I Tsp509I XhoII