(Circular) (Six-base) MAPSORT of: pFN21AE2720.seq Check: 3127 from: 1 to: 5629 pFN21AE2720 5629 bp With 182 enzymes: SgfI * June 6, 2018 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2522 2522 Size: 5629 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2688 2972 1937 Size: 751 284 4594 Fragments arranged by size: 4594 751 284 Acc65I G'GTAC_C Cuts at: 2840 2950 2840 Size: 110 5519 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2582 3692 5076 5344 1632 Size: 950 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 950 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 2254 4528 5505 1203 Size: 1051 2274 977 1327 Fragments arranged by size: 2274 1327 1051 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2456 2854 2913 2938 3164 3316 5425 1493 Size: 963 398 59 25 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 398 226 152 59 25 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2711 2840 2950 4405 618 Size: 325 217 627 924 129 110 1455 1842 Fragments arranged by size: 1842 1455 924 627 325 217 129 110 BanII G_rGCy'C Cuts at: 1391 2382 2948 1391 Size: 991 566 4072 Fragments arranged by size: 4072 991 566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 928 Size: 971 285 4373 Fragments arranged by size: 4373 971 285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3820 3854 1903 Size: 34 50 34 1799 34 3678 Fragments arranged by size: 3678 1799 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2202 3521 4731 2202 Size: 1319 1210 3100 Fragments arranged by size: 3100 1319 1210 BclI T'GATC_A Cuts at: 1548 2113 2227 1548 Size: 565 114 4950 Fragments arranged by size: 4950 565 114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2560 2810 5624 2560 Size: 250 2814 2565 Fragments arranged by size: 2814 2565 250 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 3690 4846 1790 Size: 1900 1156 2573 Fragments arranged by size: 2573 1900 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4289 1239 Size: 3050 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2203 3754 4619 4917 2203 Size: 1551 865 298 2915 Fragments arranged by size: 2915 1551 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 4062 4460 4734 4881 5292 2205 Size: 1857 398 274 147 411 2542 Fragments arranged by size: 2542 1857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2365 2368 1186 Size: 1179 3 4447 Fragments arranged by size: 4447 1179 3 BseYI C'CCAG_C Cuts at: 2715 4832 5328 2715 Size: 2117 496 3016 Fragments arranged by size: 3016 2117 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2878 1163 Size: 573 1142 3914 Fragments arranged by size: 3914 1142 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2382 3690 3775 4846 2382 Size: 1308 85 1071 3165 Fragments arranged by size: 3165 1308 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2948 3690 3775 4846 1391 Size: 399 592 566 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 566 399 85 BspEI T'CCGG_A Cuts at: 2205 5292 2205 Size: 3087 2542 BspHI T'CATG_A Cuts at: 2338 2697 3518 5248 2338 Size: 359 821 1730 2719 Fragments arranged by size: 2719 1730 821 359 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 2984 844 Size: 1678 462 3489 Fragments arranged by size: 3489 1678 462 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2632 2143 Size: 489 5140 BstYI r'GATC_y Cuts at: 1972 2500 2560 2810 2959 3326 3711 3728 5180 Size: 528 60 250 149 367 385 17 1452 Cuts at: 5180 5266 5278 5624 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 528 385 367 346 250 149 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 2348 2348 Size: 5629 BtgI C'CryG_G Cuts at: 513 1065 2012 513 Size: 552 947 4130 Fragments arranged by size: 4130 947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 3145 3939 3959 3145 Size: 794 20 4815 Fragments arranged by size: 4815 794 20 ClaI AT'CG_AT Cuts at: 1554 2901 1554 Size: 1347 4282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2638 2673 529 Size: 35 2074 35 3485 Fragments arranged by size: 3485 2074 35 35 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DraIII CAC_nnn'GTG Cuts at: 2923 2923 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2009 2193 3076 Size: 54 1425 50 275 18 179 184 883 Cuts at: 3076 3965 5594 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 883 275 184 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2242 2259 3559 1353 Size: 889 17 1300 3423 Fragments arranged by size: 3423 1300 889 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2380 2380 Size: 5629 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2582 3692 4289 5076 5344 1239 Size: 393 950 1110 597 787 268 1524 Fragments arranged by size: 1524 1110 950 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2061 2219 2061 Size: 158 5471 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2288 2288 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2752 4776 1678 Size: 1074 2024 2531 Fragments arranged by size: 2531 2024 1074 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2802 2834 4270 4302 4344 Size: 32 135 32 1230 32 1436 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 1436 1230 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 2150 748 Size: 1402 4227 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2844 2954 2844 Size: 110 5519 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 2254 5505 2254 Size: 3251 2378 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2012 4743 4927 1305 Size: 707 2731 184 2007 Fragments arranged by size: 2731 2007 707 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2451 3587 3946 4105 518 Size: 648 1285 1136 359 159 2042 Fragments arranged by size: 2042 1285 1136 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2887 3045 3722 4870 5115 1606 Size: 975 306 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 677 306 245 158 NcoI C'CATG_G Cuts at: 513 1065 2012 513 Size: 552 947 4130 Fragments arranged by size: 4130 947 552 NdeI CA'TA_TG Cuts at: 387 2707 387 Size: 2320 3309 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2450 2802 2987 4532 1132 Size: 97 1221 352 185 1545 2229 Fragments arranged by size: 2229 1545 1221 352 185 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2018 2683 1108 Size: 910 665 4054 Fragments arranged by size: 4054 910 665 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2061 2061 Size: 5629 PshAI GACnn'nnGTC Cuts at: 1613 2833 1613 Size: 1220 4409 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PstI C_TGCA'G Cuts at: 830 2618 2981 830 Size: 1788 363 3478 Fragments arranged by size: 3478 1788 363 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2887 2887 Size: 5629 SacI G_AGCT'C Cuts at: 2382 2382 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SapI GCTCTTCn'nnn_ Cuts at: 2259 2259 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2241 2393 2614 2977 4112 4793 4984 Size: 219 1196 152 221 363 1135 681 191 Cuts at: 4984 826 Size: 1471 Fragments arranged by size: 1471 1196 1135 681 363 221 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2182 3733 4634 4896 820 Size: 197 926 239 1551 901 262 1553 Fragments arranged by size: 1553 1551 926 901 262 239 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2785 1517 Size: 1268 4361 StyI C'CwwG_G Cuts at: 513 1065 2012 2607 3068 513 Size: 552 947 595 461 3074 Fragments arranged by size: 3074 947 595 552 461 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 3656 3673 3826 4011 4456 325 Size: 1255 1149 927 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1149 927 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2323 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 2574 3758 2574 Size: 1184 4445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI