Restriction map for
pFN21AE2733
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 184 GGTACC G^GTACC
AccI 586 GTCTAC GT^[AC][GT]AC
AccII 704 CGCG CG^CG
AcsI 134 GAATTT [GA]^AATT[CT]
AcyI 268 GGCGTC G[GA]^CG[CT]C
AhdI 952 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 673 CCTGAGG CC^T[ACGT]AGG
ApaI 23 GGGCCC GGGCC^C
ApoI 134 GAATTT [GA]^AATT[CT]
Asp718I 184 GGTACC G^GTACC
AspEI 952 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI 562 CTAG C^TAG
BsaHI 268 GGCGTC G[GA]^CG[CT]C
Bsp1407I 11 TGTACA T^GTACA
BspMI 596 ACCTGC ACCTGC(4/8)
BsrGI 11 TGTACA T^GTACA
BssSI 928 CACGAG CACGAG(-5/-1)
BstAPI 824 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 704 CGCG CG^CG
BstXI 103 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 673 CCTGAGG CC^T[ACGT]AGG
DsaI 491 CCGTGG C^C[GA][CT]GG
Eam1105I 952 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 673 CCTGAGG CC^T[ACGT]AGG
EcoT14I 908 CCAAGG C^C[AT][AT]GG
HaeII 773 GGCGCT [GA]GCGC^[CT]
HgaI 269 GCGTC GACGC(5/10)
Hin1I 268 GGCGTC G[GA]^CG[CT]C
KpnI 184 GGTACC GGTAC^C
MaeI 562 CTAG C^TAG
MslI 943 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 704 CGCG CG^CG
PflMI 1008 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 579 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 423 CAGCTG CAG^CTG
SacI 328 GAGCTC GAGCT^C
SbfI 429 CCTGCAGG CCTGCA^GG
Sse8387I 429 CCTGCAGG CCTGCA^GG
SspBI 11 TGTACA T^GTACA
StyI 908 CCAAGG C^C[AT][AT]GG
TaqI 983 TCGA T^CGA
Tsp509I 135 AATT ^AATT
TthHB8I 983 TCGA T^CGA
Van91I 1008 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1004 CCATCCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 396 GATCC GGATC(4/5)
827 GATCC
BanI 184 GGTACC G^G[CT][GA]CC
793 GGCACC
BglI 886 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1018 GCCCGTCCGGC
BsaI 99 GAGACC GGTCTC(1/5)
496 GAGACC
BsmBI 270 CGTCTC CGTCTC(1/5)
839 GAGACG
BstYI 395 AGATCC [GA]^GATC[CT]
826 AGATCC
DpnII 396 GATC ^GATC
827 GATC
MaeII 734 ACGT A^CGT
842 ACGT
MboI 396 GATC ^GATC
827 GATC
MboII 288 GAAGA GAAGA(8/7)
62 TCTTC
MflI 395 AGATCC [GA]^GATC[CT]
826 AGATCC
NdeII 396 GATC ^GATC
827 GATC
PpuMI 406 GGGACCT [GA]G^G[AT]CC[CT]
669 GGGTCCT
Sau3AI 396 GATC ^GATC
827 GATC
SfaNI 194 GATGC GCATC(5/9)
989 GCATC
XhoII 395 AGATCC [GA]^GATC[CT]
826 AGATCC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MnlI MspA1I MspI
MvaI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PleI PstI RsaI
Sau96I ScrFI SfcI SmaI TseI
Tsp45I TspRI XmaCI XmaI