(Circular) (Six-base) MAPSORT of: pFN21AE2733.seq Check: 5385 from: 1 to: 5749 pFN21AE2733 5749 bp With 182 enzymes: SgfI * September 11, 2018 19:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2610 2610 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 3092 1937 Size: 655 500 4594 Fragments arranged by size: 4594 655 500 Acc65I G'GTAC_C Cuts at: 2189 3070 2189 Size: 881 4868 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 5196 5464 1632 Size: 2180 1384 268 1917 Fragments arranged by size: 2180 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 2962 4478 2962 Size: 1516 4233 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2064 1184 Size: 880 4869 AlwNI CAG_nnn'CTG Cuts at: 2150 2176 2695 2835 2861 3492 5064 2150 Size: 26 519 140 26 631 1572 2835 Fragments arranged by size: 2835 1572 631 519 140 26 26 ApaI G_GGCC'C Cuts at: 2032 2032 Size: 5749 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 2139 3058 3284 3436 5545 1493 Size: 646 919 226 152 2109 1697 Fragments arranged by size: 2109 1697 919 646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2086 2358 2463 2515 2810 3074 1870 Size: 73 143 272 105 52 295 264 4545 Fragments arranged by size: 4545 295 272 264 143 105 73 52 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2724 2757 2724 Size: 33 5716 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 2798 3070 4525 618 Size: 325 217 627 402 609 272 1455 1842 Fragments arranged by size: 1842 1455 627 609 402 325 272 217 BanII G_rGCy'C Cuts at: 1391 2032 2337 2478 2940 3068 1391 Size: 641 305 141 462 128 4072 Fragments arranged by size: 4072 641 462 305 141 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2897 3029 4360 Size: 107 122 71 631 605 1225 132 1331 Cuts at: 4360 136 Size: 1525 Fragments arranged by size: 1525 1331 1225 631 605 132 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2032 3810 4966 1790 Size: 242 1778 1156 2573 Fragments arranged by size: 2573 1778 1156 242 BmgBI CAC'GTC Cuts at: 1318 2740 2848 1318 Size: 1422 108 4219 Fragments arranged by size: 4219 1422 108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2186 2255 2400 Size: 416 53 83 399 24 769 69 145 Cuts at: 2400 3480 4438 442 Size: 1080 958 1753 Fragments arranged by size: 1753 1080 958 769 416 399 145 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 2437 2981 4409 1239 Size: 1171 27 544 1428 2579 Fragments arranged by size: 2579 1428 1171 544 27 Bpu10I CC'TnA_GC Cuts at: 2522 2839 2522 Size: 317 5432 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2098 2495 4412 882 Size: 617 599 397 1917 2219 Fragments arranged by size: 2219 1917 617 599 397 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 3556 3938 4573 Size: 53 83 186 936 741 1282 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1282 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2173 2203 2526 2556 1178 Size: 30 165 30 770 30 323 30 4371 Fragments arranged by size: 4371 770 323 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2486 2552 2635 1186 Size: 1300 66 83 4300 Fragments arranged by size: 4300 1300 83 66 BseYI C'CCAG_C Cuts at: 2205 2255 2791 2854 2882 4952 5448 2205 Size: 50 536 63 28 2070 496 2506 Fragments arranged by size: 2506 2070 536 496 63 50 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2477 2639 2930 1163 Size: 573 498 243 162 291 3982 Fragments arranged by size: 3982 573 498 291 243 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 2426 2982 3810 3895 4966 2337 Size: 89 556 828 85 1071 3120 Fragments arranged by size: 3120 1071 828 556 89 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 BsmBI CGTCTCn'nnnn_ Cuts at: 2281 2838 2281 Size: 557 5192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2337 2426 2478 2940 2982 3068 Size: 399 242 305 89 52 462 42 86 Cuts at: 3068 3810 3895 4966 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 305 242 89 86 85 52 42 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2610 3104 844 Size: 1766 494 3489 Fragments arranged by size: 3489 1766 494 BsrBI CCG'CTC Cuts at: 1394 1823 2392 2509 2942 3636 1394 Size: 429 569 117 433 694 3507 Fragments arranged by size: 3507 694 569 433 429 117 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2570 2605 2795 4393 4580 1700 Size: 110 760 35 190 1598 187 2869 Fragments arranged by size: 2869 1598 760 190 187 110 35 BsrGI T'GTAC_A Cuts at: 96 2016 96 Size: 1920 3829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 2933 3809 4821 2933 Size: 876 1012 3861 Fragments arranged by size: 3861 1012 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 2835 794 Size: 2041 3708 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2115 2011 Size: 104 5645 BstYI r'GATC_y Cuts at: 1972 2400 2831 3079 3446 3831 3848 5300 5386 Size: 428 431 248 367 385 17 1452 86 Cuts at: 5386 5398 5744 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 431 428 385 367 346 248 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2679 2679 Size: 5749 BtgI C'CryG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3766 Fragments arranged by size: 3766 1431 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2755 2922 3008 3265 4059 4079 2755 Size: 167 86 257 794 20 4425 Fragments arranged by size: 4425 794 257 167 86 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714 Size: 54 1425 50 275 18 1366 889 1629 Cuts at: 5714 8 Size: 43 Fragments arranged by size: 1629 1425 1366 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2601 4336 4710 4856 387 Size: 1064 138 1012 1735 374 146 1280 Fragments arranged by size: 1735 1280 1064 1012 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 2335 Size: 5749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2437 2981 3812 4409 5196 5464 Size: 393 778 27 544 831 597 787 268 Cuts at: 5464 1239 Size: 1524 Fragments arranged by size: 1524 831 787 778 597 544 393 268 27 EcoO109I rG'GnC_Cy Cuts at: 2028 2029 2147 2412 2675 2028 Size: 1 118 265 263 5102 Fragments arranged by size: 5102 265 263 118 1 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2782 4896 1678 Size: 1104 2114 2531 Fragments arranged by size: 2531 2114 1104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2948 2980 4390 4422 4464 Size: 32 135 32 1376 32 1410 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 1376 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 2193 3074 2193 Size: 881 4868 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2081 4863 5047 1305 Size: 776 2782 184 2007 Fragments arranged by size: 2782 2007 776 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2952 3707 4066 4225 518 Size: 648 1786 755 359 159 2042 Fragments arranged by size: 2042 1786 755 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2430 2629 2765 3165 3842 4990 5235 1606 Size: 824 199 136 400 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 400 245 199 136 NaeI GCC'GGC Cuts at: 2572 2607 2797 2572 Size: 35 190 5524 Fragments arranged by size: 5524 190 35 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NgoMIV G'CCGG_C Cuts at: 2570 2605 2795 2570 Size: 35 190 5524 Fragments arranged by size: 5524 190 35 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 2903 1348 Size: 1555 4194 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 3019 1108 Size: 1911 3838 PfoI T'CCnGG_A Cuts at: 2394 2448 2394 Size: 54 5695 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2412 2675 2412 Size: 263 5486 PshAI GACnn'nnGTC Cuts at: 1613 2588 1613 Size: 975 4774 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2028 2028 Size: 5749 PstI C_TGCA'G Cuts at: 830 2439 2623 2831 3003 3101 830 Size: 1609 184 208 172 98 3478 Fragments arranged by size: 3478 1609 208 184 172 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2430 2430 Size: 5749 SacI G_AGCT'C Cuts at: 2337 2337 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 2439 3101 2439 Size: 662 5087 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2435 2619 2827 2999 3097 4232 4913 Size: 219 1390 184 208 172 98 1135 681 Cuts at: 4913 5104 826 Size: 191 1471 Fragments arranged by size: 1471 1390 1135 681 219 208 191 184 172 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2088 2465 2517 2812 3076 1872 Size: 216 377 52 295 264 4545 Fragments arranged by size: 4545 377 295 264 216 52 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2913 3188 513 Size: 552 1848 275 3074 Fragments arranged by size: 3074 1848 552 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 2750 3995 Size: 274 80 33 51 1443 39 734 1245 Cuts at: 3995 5707 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1245 734 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3016 3016 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2086 2463 2515 2810 3074 1870 Size: 216 377 52 295 264 4545 Fragments arranged by size: 4545 377 295 264 216 52 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI