(Linear) (Six-base) MAPSORT of: KIEE2733.seq Check: 3681 from: 1 to: 1044 KIEE2733.orf With 182 enzymes: SgfI * September 11, 2018 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 605 1044 Size: 605 439 AccI GT'mk_AC Cuts at: 0 587 1044 Size: 587 457 Acc65I G'GTAC_C Cuts at: 0 184 1044 Size: 184 860 AhdI GACnn_n'nnGTC Cuts at: 0 957 1044 Size: 957 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 59 1044 Size: 59 985 AlwNI CAG_nnn'CTG Cuts at: 0 145 171 690 830 856 1044 Size: 145 26 519 140 26 188 Fragments arranged by size: 519 188 145 140 26 26 ApaI G_GGCC'C Cuts at: 0 27 1044 Size: 27 1017 ApoI r'AATT_y Cuts at: 0 134 1044 Size: 134 910 AvaI C'yCGr_G Cuts at: 0 81 353 458 510 805 1044 Size: 81 272 105 52 295 239 Fragments arranged by size: 295 272 239 105 81 52 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 719 752 1044 Size: 719 33 292 Fragments arranged by size: 719 292 33 BanI G'GyrC_C Cuts at: 0 184 793 1044 Size: 184 609 251 Fragments arranged by size: 609 251 184 BanII G_rGCy'C Cuts at: 0 27 332 473 935 1044 Size: 27 305 141 462 109 Fragments arranged by size: 462 305 141 109 27 BglI GCCn_nnn'nGGC Cuts at: 0 892 1024 1044 Size: 892 132 20 Fragments arranged by size: 892 132 20 Bme1580I G_kGCm'C Cuts at: 0 27 1044 Size: 27 1017 BmgBI CAC'GTC Cuts at: 0 735 843 1044 Size: 735 108 201 Fragments arranged by size: 735 201 108 BmrI ACTGGGnnnn_n' Cuts at: 0 181 250 395 1044 Size: 181 69 145 649 Fragments arranged by size: 649 181 145 69 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 432 976 1044 Size: 405 27 544 68 Fragments arranged by size: 544 405 68 27 Bpu10I CC'TnA_GC Cuts at: 0 517 834 1044 Size: 517 317 210 Fragments arranged by size: 517 317 210 BsaI GGTCTCn'nnnn_ Cuts at: 0 93 490 1044 Size: 93 397 554 Fragments arranged by size: 554 397 93 BsaHI Gr'CG_yC Cuts at: 0 269 1044 Size: 269 775 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 168 198 521 551 1044 Size: 168 30 323 30 493 Fragments arranged by size: 493 323 168 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 481 547 630 1044 Size: 481 66 83 414 Fragments arranged by size: 481 414 83 66 BseYI C'CCAG_C Cuts at: 0 200 250 786 849 877 1044 Size: 200 50 536 63 28 167 Fragments arranged by size: 536 200 167 63 50 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 472 634 925 1044 Size: 229 243 162 291 119 Fragments arranged by size: 291 243 229 162 119 BsiHKAI G_wGCw'C Cuts at: 0 332 421 977 1044 Size: 332 89 556 67 Fragments arranged by size: 556 332 89 67 BsmBI CGTCTCn'nnnn_ Cuts at: 0 276 833 1044 Size: 276 557 211 Fragments arranged by size: 557 276 211 Bsp1286I G_dGCh'C Cuts at: 0 27 332 421 473 935 977 1044 Size: 27 305 89 52 462 42 67 Fragments arranged by size: 462 305 89 67 52 42 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 605 1044 Size: 605 439 BsrBI CCG'CTC Cuts at: 0 387 504 937 1044 Size: 387 117 433 107 Fragments arranged by size: 433 387 117 107 BsrFI r'CCGG_y Cuts at: 0 565 600 790 1044 Size: 565 35 190 254 Fragments arranged by size: 565 254 190 35 BsrGI T'GTAC_A Cuts at: 0 11 1044 Size: 11 1033 BssSI C'ACGA_G Cuts at: 0 928 1044 Size: 928 116 BstAPI GCAn_nnn'nTGC Cuts at: 0 830 1044 Size: 830 214 BstXI CCAn_nnnn'nTGG Cuts at: 0 110 1044 Size: 110 934 BstYI r'GATC_y Cuts at: 0 395 826 1044 Size: 395 431 218 Fragments arranged by size: 431 395 218 Bsu36I CC'TnA_GG Cuts at: 0 674 1044 Size: 674 370 BtgI C'CryG_G Cuts at: 0 491 1044 Size: 491 553 BtsI GCAGTG_nn' Cuts at: 0 750 917 1003 1044 Size: 750 167 86 41 Fragments arranged by size: 750 167 86 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 596 1044 Size: 596 448 EcoICRI GAG'CTC Cuts at: 0 330 1044 Size: 330 714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 432 976 1044 Size: 405 27 544 68 Fragments arranged by size: 544 405 68 27 EcoO109I rG'GnC_Cy Cuts at: 0 23 24 142 407 670 1044 Size: 23 1 118 265 263 374 Fragments arranged by size: 374 265 263 118 23 1 HaeII r_GCGC'y Cuts at: 0 777 1044 Size: 777 267 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 943 975 1044 Size: 943 32 69 Fragments arranged by size: 943 69 32 KpnI G_GTAC'C Cuts at: 0 188 1044 Size: 188 856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 76 1044 Size: 76 968 MslI CAynn'nnrTG Cuts at: 0 947 1044 Size: 947 97 MspA1I CmG'CkG Cuts at: 0 425 624 760 1044 Size: 425 199 136 284 Fragments arranged by size: 425 284 199 136 NaeI GCC'GGC Cuts at: 0 567 602 792 1044 Size: 567 35 190 252 Fragments arranged by size: 567 252 190 35 NgoMIV G'CCGG_C Cuts at: 0 565 600 790 1044 Size: 565 35 190 254 Fragments arranged by size: 565 254 190 35 PasI CC'CwG_GG Cuts at: 0 898 1044 Size: 898 146 PflMI CCAn_nnn'nTGG Cuts at: 0 1014 1044 Size: 1014 30 PfoI T'CCnGG_A Cuts at: 0 389 443 1044 Size: 389 54 601 Fragments arranged by size: 601 389 54 PpuMI rG'GwC_Cy Cuts at: 0 407 670 1044 Size: 407 263 374 Fragments arranged by size: 407 374 263 PshAI GACnn'nnGTC Cuts at: 0 583 1044 Size: 583 461 PspOMI G'GGCC_C Cuts at: 0 23 1044 Size: 23 1021 PstI C_TGCA'G Cuts at: 0 434 618 826 998 1044 Size: 434 184 208 172 46 Fragments arranged by size: 434 208 184 172 46 PvuII CAG'CTG Cuts at: 0 425 1044 Size: 425 619 SacI G_AGCT'C Cuts at: 0 332 1044 Size: 332 712 SbfI CC_TGCA'GG Cuts at: 0 434 1044 Size: 434 610 SfcI C'TryA_G Cuts at: 0 430 614 822 994 1044 Size: 430 184 208 172 50 Fragments arranged by size: 430 208 184 172 50 SmaI CCC'GGG Cuts at: 0 83 460 512 807 1044 Size: 83 377 52 295 237 Fragments arranged by size: 377 295 237 83 52 StyI C'CwwG_G Cuts at: 0 908 1044 Size: 908 136 TatI w'GTAC_w Cuts at: 0 11 745 1044 Size: 11 734 299 Fragments arranged by size: 734 299 11 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1011 1044 Size: 1011 33 XmaI C'CCGG_G Cuts at: 0 81 458 510 805 1044 Size: 81 377 52 295 239 Fragments arranged by size: 377 295 239 81 52 Enzymes that do cut: AarI AccI Acc65I AhdI AlfI AlwNI ApaI ApoI AvaI BaeI BanI BanII BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SbfI SfcI SmaI StyI TatI XcmI XmaI Enzymes that do not cut: AatII AclI AcuI AfeI AflII AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI