(Circular) (Six-base) MAPSORT of: pFN21AE6078.seq Check: 1927 from: 1 to: 5674 pFN21AE6078 5674 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2231 3017 1937 Size: 294 786 4594 Fragments arranged by size: 4594 786 294 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 3737 5121 5389 1632 Size: 887 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 887 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2821 4573 5550 1203 Size: 1618 1752 977 1327 Fragments arranged by size: 1752 1618 1327 977 AgeI A'CCGG_T Cuts at: 2925 4505 2925 Size: 1580 4094 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 2240 2745 1166 Size: 1074 505 4095 Fragments arranged by size: 4095 1074 505 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 3417 4989 3417 Size: 1572 4102 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2999 1870 Size: 73 1056 4545 Fragments arranged by size: 4545 1056 73 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2995 4450 618 Size: 325 217 627 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 627 325 217 BanII G_rGCy'C Cuts at: 1391 2972 2993 1391 Size: 1581 21 4072 Fragments arranged by size: 4072 1581 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2900 928 Size: 971 1001 3702 Fragments arranged by size: 3702 1001 971 BbvCI CC'TCA_GC Cuts at: 2723 2723 Size: 5674 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2451 2485 3865 3899 1903 Size: 34 514 34 1380 34 3678 Fragments arranged by size: 3678 1380 514 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 3091 3091 Size: 5674 Bme1580I G_kGCm'C Cuts at: 1790 2184 2247 2395 2619 3735 4891 1790 Size: 394 63 148 224 1116 1156 2573 Fragments arranged by size: 2573 1156 1116 394 224 148 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2477 3405 4363 Size: 416 53 83 399 24 1060 928 958 Cuts at: 4363 442 Size: 1753 Fragments arranged by size: 1753 1060 958 928 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2460 2460 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4334 1239 Size: 3095 2579 Bpu10I CC'TnA_GC Cuts at: 2723 2723 Size: 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3799 4664 4962 3799 Size: 865 298 4511 Fragments arranged by size: 4511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2909 3465 493 Size: 711 1705 556 2702 Fragments arranged by size: 2702 1705 711 556 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2925 4107 4505 4779 4926 5337 2925 Size: 1182 398 274 147 411 3262 Fragments arranged by size: 3262 1182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2658 2688 1178 Size: 30 165 30 1255 30 4164 Fragments arranged by size: 4164 1255 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5674 BseYI C'CCAG_C Cuts at: 4877 5373 4877 Size: 496 5178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2247 2395 2619 2972 2993 3735 Size: 399 394 63 148 224 353 21 742 Cuts at: 3735 3820 4891 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 353 224 148 85 63 21 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2283 4169 4343 65 Size: 1183 1035 1886 174 1396 Fragments arranged by size: 1886 1396 1183 1035 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2925 4318 4505 1700 Size: 110 1115 1393 187 2869 Fragments arranged by size: 2869 1393 1115 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2626 2801 2626 Size: 175 5499 BstYI r'GATC_y Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669 Size: 1032 367 385 17 1452 86 12 346 Cuts at: 5669 1972 Size: 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2361 513 Size: 552 1296 3826 Fragments arranged by size: 3826 1296 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2635 487 Size: 683 56 1409 3526 Fragments arranged by size: 3526 1409 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2688 2862 3190 3984 4004 2118 Size: 570 174 328 794 20 3788 Fragments arranged by size: 3788 794 570 328 174 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2382 2448 Size: 54 1425 50 275 18 378 174 66 Cuts at: 2448 2838 3121 4010 5639 8 Size: 390 283 889 1629 43 Fragments arranged by size: 1629 1425 889 390 378 283 275 174 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 3737 4334 5121 5389 1239 Size: 393 887 1218 597 787 268 1524 Fragments arranged by size: 1524 1218 887 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2251 2719 2251 Size: 468 5206 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373 Size: 32 135 32 2743 32 42 32 2626 Fragments arranged by size: 2743 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 3018 3264 669 Size: 988 281 655 425 246 3079 Fragments arranged by size: 3079 988 655 425 281 246 HindIII A'AGCT_T Cuts at: 748 2464 748 Size: 1716 3958 HpaI GTT'AAC Cuts at: 2593 3264 2593 Size: 671 5003 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 4788 4972 1305 Size: 744 2739 184 2007 Fragments arranged by size: 2739 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 2840 10 Size: 54 1425 343 552 456 2844 Fragments arranged by size: 2844 1425 552 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2016 2240 2624 2654 2745 2756 3632 Size: 648 850 224 384 30 91 11 876 Cuts at: 3632 3991 4150 518 Size: 359 159 2042 Fragments arranged by size: 2042 876 850 648 384 359 224 159 91 30 11 MspA1I CmG'CkG Cuts at: 1606 3090 3767 4915 5160 1606 Size: 1484 677 1148 245 2120 Fragments arranged by size: 2120 1484 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2361 513 Size: 552 1296 3826 Fragments arranged by size: 3826 1296 552 NdeI CA'TA_TG Cuts at: 387 2325 387 Size: 1938 3736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2825 3032 4577 1132 Size: 97 1596 207 1545 2229 Fragments arranged by size: 2229 1596 1545 207 97 PasI CC'CwG_GG Cuts at: 1348 2063 1348 Size: 715 4959 PciI A'CATG_T Cuts at: 2821 4573 2821 Size: 1752 3922 PflMI CCAn_nnn'nTGG Cuts at: 1108 2265 1108 Size: 1157 4517 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 2041 2125 3026 830 Size: 1211 84 901 3478 Fragments arranged by size: 3478 1211 901 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2037 2121 3022 4157 4838 5029 826 Size: 219 992 84 901 1135 681 191 1471 Fragments arranged by size: 1471 1135 992 901 681 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3778 4679 4941 820 Size: 197 926 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 2812 3598 5 Size: 47 2760 786 2081 Fragments arranged by size: 2760 2081 786 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2247 2308 2361 3113 513 Size: 552 1182 61 53 752 3074 Fragments arranged by size: 3074 1182 752 552 61 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3701 3718 3871 4056 4501 325 Size: 1255 741 1380 17 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3920 5632 96 Size: 274 80 33 51 1443 1943 1712 138 Fragments arranged by size: 1943 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2658 2690 5634 5666 2658 Size: 32 2944 32 2666 Fragments arranged by size: 2944 2666 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2848 2848 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI