################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 670 CTTAAG C^TTAAG AflIII 691 ACACGT A^C[GA][CT]GT BamHI 6 GGATCC G^GATCC BanI 474 GGCACC G^G[CT][GA]CC BbrPI 692 CACGTG CAC^GTG BcnI 582 CCGGG CC^[GC]GG BfaI 124 CTAG C^TAG BfrI 670 CTTAAG C^TTAAG BsaAI 692 CACGTG [CT]AC^GT[GA] BsgI 464 CTGCAC GTGCAG(16/14) BsmAI 727 GTCTC GTCTC(1/5) BsmBI 726 CGTCTC CGTCTC(1/5) BspHI 557 TCATGA T^CATGA BsrBI 513 CCGCTC CCGCTC(-3/-3) BsrDI 17 GCAATG GCAATG(2/0) BssSI 422 CTCGTG CACGAG(-5/-1) BstAPI 162 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 6 GGATCC [GA]^GATC[CT] DpnII 7 GATC ^GATC EarI 67 GAAGAG CTCTTC(1/4) EcoRV 577 GATATC GAT^ATC FauI 456 GCGGG CCCGC(4/6) HapII 582 CCGG C^CGG HgaI 725 GCGTC GACGC(5/10) HpaII 582 CCGG C^CGG Ksp632I 67 GAAGAG CTCTTC(1/4) MaeI 124 CTAG C^TAG MboI 7 GATC ^GATC MfeI 869 CAATTG C^AATTG MflI 6 GGATCC [GA]^GATC[CT] MseI 671 TTAA T^TAA MspI 582 CCGG C^CGG MunI 869 CAATTG C^AATTG NciI 582 CCGGG CC^[GC]GG NdeII 7 GATC ^GATC NspI 197 GCATGC [GA]CATG^[CT] PflMI 254 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 692 CACGTG CAC^GTG PmlI 692 CACGTG CAC^GTG PvuII 499 CAGCTG CAG^CTG RcaI 557 TCATGA T^CATGA Sau3AI 7 GATC ^GATC ScaI 408 AGTACT AGT^ACT SfcI 301 CTACAG C^T[GA][CT]AG SmlI 670 CTTAAG C^T[CT][GA]AG SphI 197 GCATGC GCATG^C StuI 51 AGGCCT AGG^CCT Tru9I 671 TTAA T^TAA Van91I 254 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 6 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 409 GTAC GT^AC 760 GTAC AluI 500 AGCT AG^CT 563 AGCT AlwI 7 GATCC GGATC(4/5) 6 GGATC BanII 175 GAGCCC G[GA]GC[CT]^C 946 GAGCCC BbsI 885 GAAGAC GAAGAC(2/6) 487 GTCTTC BpuAI 885 GAAGAC GAAGAC(2/6) 487 GTCTTC BsrI 26 CCAGT ACTGG(1/-1) 282 CCAGT BstXI 242 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 623 CCATTGGCCTGG CfoI 396 GCGC GCG^C 906 GCGC DdeI 74 CTCAG C^T[ACGT]AG 158 CTGAG DsaI 356 CCATGG C^C[GA][CT]GG 779 CCATGG HhaI 396 GCGC GCG^C 906 GCGC HinP1I 396 GCGC G^CGC 906 GCGC HphI 387 TCACC GGTGA(8/7) 428 TCACC MaeII 415 ACGT A^CGT 693 ACGT MslI 547 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 733 CATGTGTGTG MspA1I 499 CAGCTG C[AC]G^C[GT]G 814 CCGCTG NcoI 356 CCATGG C^CATGG 779 CCATGG PleI 634 GACTC GAGTC(4/5) 808 GACTC RsaI 409 GTAC GT^AC 760 GTAC TaqI 900 TCGA T^CGA 915 TCGA TthHB8I 900 TCGA T^CGA 915 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AspHI BalI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboII MluNI MnlI MscI MvaI MwoI NlaIII NlaIV ScrFI SfaNI StyI TfiI TseI Tsp45I Tsp509I TspRI XcmI