Restriction map for
pFN21AB7131
(Circular) (Six-base) MAPSORT of: pFN21AB7131.seq Check: 8890 from: 1 to: 5656
pFN21AB7131 5656 bp
With 182 enzymes: SgfI *
March 10, 2016 11:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2931 3719 5103 5371 1632
Size: 1299 788 1384 268 1917
Fragments arranged by size:
1917 1384 1299 788 268
AflII C'TTAA_G
Cuts at: 820 1017 2675 820
Size: 197 1658 3801
Fragments arranged by size:
3801 1658 197
AflIII A'CryG_T
Cuts at: 1203 2696 4555 5532 1203
Size: 1493 1859 977 1327
Fragments arranged by size:
1859 1493 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2011 2986 3353 2011
Size: 975 367 4314
Fragments arranged by size:
4314 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2479 2977 4432 618
Size: 325 217 627 692 498 1455 1842
Fragments arranged by size:
1842 1455 692 627 498 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 2955 2975 1391
Size: 793 771 20 4072
Fragments arranged by size:
4072 793 771 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2485 2897 928
Size: 971 586 412 3687
Fragments arranged by size:
3687 971 586 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2699 3447 493
Size: 711 1495 748 2702
Fragments arranged by size:
2702 1495 748 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 4859 5355 4859
Size: 496 5160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2454 1163
Size: 573 718 4365
Fragments arranged by size:
4365 718 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2169 2868 3717 3802 4873 2108
Size: 61 699 849 85 1071 2891
Fragments arranged by size:
2891 1071 849 699 85 61
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2737 2737
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2169 2184 2868 2955 2975 3717
Size: 399 318 61 15 684 87 20 742
Cuts at: 3717 3802 4873 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 399 318 87 85
61 20 15
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2562 3545 5275 2562
Size: 983 1730 2943
Fragments arranged by size:
2943 1730 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2520 3543 1394
Size: 429 697 1023 3507
Fragments arranged by size:
3507 1023 697 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2029 4151 4325 65
Size: 1183 781 2122 174 1396
Fragments arranged by size:
2122 1396 1183 781 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2427 3716 4728 2427
Size: 1289 1012 3355
Fragments arranged by size:
3355 1289 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 794
Size: 1379 4277
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2635 2254
Size: 381 5275
BstYI r'GATC_y
Cuts at: 1972 2011 2986 3353 3738 3755 5207 5293 5305
Size: 39 975 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 39
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2784 513
Size: 552 939 357 423 3385
Fragments arranged by size:
3385 939 552 423 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2777 487
Size: 683 56 1551 3366
Fragments arranged by size:
3366 1551 683 56
BtsI GCAGTG_nn'
Cuts at: 2396 3172 3966 3986 2396
Size: 776 794 20 4066
Fragments arranged by size:
4066 794 776 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2358 2382
Size: 54 1425 50 275 18 426 102 24
Cuts at: 2382 2835 2934 3103 3992 5621 8
Size: 453 99 169 889 1629 43
Fragments arranged by size:
1629 1425 889 453 426 275 169 102
99 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3586 1353
Size: 714 1519 3423
Fragments arranged by size:
3423 1519 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2614 4243 4617 4763 387
Size: 1064 138 1025 1629 374 146 1280
Fragments arranged by size:
1629 1280 1064 1025 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2931 3719 4316 5103 5371 1239
Size: 393 1299 788 597 787 268 1524
Fragments arranged by size:
1524 1299 788 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 5656
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2898 2930 4297 4329 4371
Size: 32 135 32 1326 32 1367 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 1367 1326 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 2874 3255 2874
Size: 381 5275
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 2360 2384 2837 2936
Size: 54 1425 343 426 102 24 453 99
Cuts at: 2936 10
Size: 2730
Fragments arranged by size:
2730 1425 453 426 343 102 99 54
24
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 2742 3614 3973 4132 518
Size: 648 1390 186 872 359 159 2042
Fragments arranged by size:
2042 1390 872 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2506 2821 3072 3749 4897 5142 1606
Size: 900 315 251 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 315 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2361 2784 513
Size: 552 939 357 423 3385
Fragments arranged by size:
3385 939 552 423 357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2206 3014 4559 1132
Size: 97 977 808 1545 2229
Fragments arranged by size:
2229 1545 977 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2265 1108
Size: 1157 4499
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PmlI CAC'GTG
Cuts at: 2699 2699
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 3725 3757 5238 5270 2066
Size: 32 1627 32 1481 32 2452
Fragments arranged by size:
2452 1627 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2506 2506
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 2415 3904 5616 2415
Size: 1489 1712 2455
Fragments arranged by size:
2455 1712 1489
SfcI C'TryA_G
Cuts at: 826 1045 2306 3004 4139 4820 5011 826
Size: 219 1261 698 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 698 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2675 3760 4661 4923 820
Size: 197 926 732 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 732 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 2206 3014 1132
Size: 1074 808 3774
Fragments arranged by size:
3774 1074 808
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2058 1517
Size: 541 5115
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2712 2784 3095 513
Size: 552 939 357 351 72 311 3074
Fragments arranged by size:
3074 939 552 357 351 311 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2409 3683 3700 3853 4038 4483 325
Size: 1255 829 1274 17 153 185 445 1498
Fragments arranged by size:
1498 1274 1255 829 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2413 3902 5614
Size: 274 80 33 51 1443 436 1489 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1712 1489 1443 436 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2353 2443 2330
Size: 23 90 5543
Fragments arranged by size:
5543 90 23
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI