################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MspA1I MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 243 GTCGAC GT^[AC][GT]AC AccIII 674 TCCGGA T^CCGGA AcsI 31 GAATTC [GA]^AATT[CT] AlwNI 70 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTC [GA]^AATT[CT] BanII 46 GGGCTC G[GA]GC[CT]^C BbsI 264 GAAGAC GAAGAC(2/6) BcnI 60 CCCGG CC^[GC]GG BfaI 118 CTAG C^TAG BmyI 46 GGGCTC G[AGT]GC[ACT]^C BpuAI 264 GAAGAC GAAGAC(2/6) BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG BseAI 674 TCCGGA T^CCGGA BsmFI 754 GGGAC GGGAC(10/14) BsmI 706 GCATTC GAATGC(1/-1) Bsp1286I 46 GGGCTC G[AGT]GC[ACT]^C BspEI 674 TCCGGA T^CCGGA BsrBI 544 CCGCTC CCGCTC(-3/-3) BstXI 632 CCATCGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 505 AGATCC [GA]^GATC[CT] CfoI 387 GCGC GCG^C EarI 466 CTCTTC CTCTTC(1/4) EcoRI 31 GAATTC G^AATTC HhaI 387 GCGC GCG^C HinP1I 387 GCGC G^CGC HincII 243 GTCGAC GT[CT]^[GA]AC HindII 243 GTCGAC GT[CT]^[GA]AC HindIII 553 AAGCTT A^AGCTT Ksp632I 466 CTCTTC CTCTTC(1/4) MaeI 118 CTAG C^TAG MaeII 403 ACGT A^CGT MflI 505 AGATCC [GA]^GATC[CT] MroI 674 TCCGGA T^CCGGA NciI 60 CCCGG CC^[GC]GG NdeI 727 CATATG CA^TATG NlaIV 721 GGTTCC GG[ACGT]^[ACGT]CC PflMI 726 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SalI 243 GTCGAC G^TCGAC SfaNI 419 GCATC GCATC(5/9) SphI 585 GCATGC GCATG^C StuI 893 AGGCCT AGG^CCT TaqI 244 TCGA T^CGA TfiI 805 GAATC G^A[AT]TC Tsp509I 32 AATT ^AATT TthHB8I 244 TCGA T^CGA Van91I 726 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 505 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 177 GTAC GT^AC 847 GTAC AlwI 506 GATCC GGATC(4/5) 677 GGATC BalI 368 TGGCCA TGG^CCA 428 TGGCCA BbvI 343 GCAGC GCAGC(8/12) 664 GCTGC BpmI 67 CTCCAG CTGGAG(16/14) 618 CTCCAG BsaWI 674 TCCGGA [AT]^CCGG[AT] 843 TCCGGT BsmAI 147 GAGAC GTCTC(1/5) 72 GTCTC BsrDI 309 CATTGC GCAATG(2/0) 346 GCAATG BstF5I 477 CATCC GGATG(2/0) 669 CATCC EaeI 368 TGGCCA [CT]^GGCC[GA] 428 TGGCCA FauI 234 CCCGC CCCGC(4/6) 540 CCCGC FokI 477 CATCC GGATG(9/13) 669 CATCC MaeIII 109 GTCAC ^GT[ACGT]AC 739 GTCAC MluNI 368 TGGCCA TGG^CCA 428 TGGCCA MscI 368 TGGCCA TGG^CCA 428 TGGCCA RsaI 177 GTAC GT^AC 847 GTAC TseI 343 GCAGC G^C[AT]GC 664 GCTGC Tsp45I 109 GTCAC ^GT[GC]AC 739 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI BsiYI BslI BsrI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NspI PleI Sau3AI ScrFI SfcI Tru9I TspRI