(Circular) (Six-base) MAPSORT of: pFN21AE5382.seq Check: 1674 from: 1 to: 5620 pFN21AE5382 5620 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2249 2963 1937 Size: 312 714 4594 Fragments arranged by size: 4594 714 312 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3683 5067 5335 1632 Size: 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2840 1166 Size: 1674 3946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 2080 3363 4935 2080 Size: 1283 1572 2765 Fragments arranged by size: 2765 1572 1283 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2036 2929 3155 3307 5416 1493 Size: 543 893 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2565 2598 2565 Size: 33 5587 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2055 2939 1391 Size: 664 884 4072 Fragments arranged by size: 4072 884 664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 928 Size: 971 377 4272 Fragments arranged by size: 4272 971 377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2608 4280 1239 Size: 818 551 1672 2579 Fragments arranged by size: 2579 1672 818 551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3745 4610 4908 3745 Size: 865 298 4457 Fragments arranged by size: 4457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2679 2848 4053 4451 4725 4872 5283 2679 Size: 169 1205 398 274 147 411 3016 Fragments arranged by size: 3016 1205 411 398 274 169 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5620 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3681 3766 4837 3681 Size: 85 1071 4464 Fragments arranged by size: 4464 1071 85 BsmI GAATG_Cn' Cuts at: 2711 3131 3224 2711 Size: 420 93 5107 Fragments arranged by size: 5107 420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2939 3681 3766 4837 1391 Size: 399 265 884 742 85 1071 2174 Fragments arranged by size: 2174 1071 884 742 399 265 85 BspEI T'CCGG_A Cuts at: 2679 5283 2679 Size: 2604 3016 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2551 3507 1394 Size: 429 728 956 3507 Fragments arranged by size: 3507 956 728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2313 2358 4115 4289 65 Size: 1183 1065 45 1757 174 1396 Fragments arranged by size: 1757 1396 1183 1065 174 45 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2644 2644 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2510 2950 3317 3702 3719 5171 5257 5269 Size: 538 440 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 538 440 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5068 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2029 3136 3930 3950 2029 Size: 1107 794 20 3699 Fragments arranged by size: 3699 1107 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2433 3067 Size: 54 1425 50 275 18 543 60 634 Cuts at: 3067 3956 5585 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 634 543 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 3550 1353 Size: 1124 1073 3423 Fragments arranged by size: 3423 1124 1073 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2483 4207 4581 4727 387 Size: 1064 138 894 1724 374 146 1280 Fragments arranged by size: 1724 1280 1064 894 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2608 3683 4280 5067 5335 1239 Size: 393 425 551 1075 597 787 268 1524 Fragments arranged by size: 1524 1075 787 597 551 425 393 268 EcoRI G'AATT_C Cuts at: 2036 2929 3307 2036 Size: 893 378 4349 Fragments arranged by size: 4349 893 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5620 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2409 2441 2502 2534 2857 Size: 32 135 32 837 32 61 32 323 Cuts at: 2857 2889 4261 4293 4335 4367 1373 Size: 32 1372 32 42 32 2626 Fragments arranged by size: 2626 1372 837 323 135 61 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2250 2964 3210 669 Size: 988 281 312 714 246 3079 Fragments arranged by size: 3079 988 714 312 281 246 HindIII A'AGCT_T Cuts at: 748 2558 748 Size: 1810 3810 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 4734 4918 1305 Size: 729 2700 184 2007 Fragments arranged by size: 2700 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 2435 10 Size: 54 1425 343 543 60 3195 Fragments arranged by size: 3195 1425 543 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2177 2840 3578 3937 4096 518 Size: 648 975 36 663 738 359 159 2042 Fragments arranged by size: 2042 975 738 663 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 3036 3713 4861 5106 1606 Size: 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5068 NdeI CA'TA_TG Cuts at: 387 2733 387 Size: 2346 3274 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2344 2594 2776 2978 4523 1132 Size: 97 1115 250 182 202 1545 2229 Fragments arranged by size: 2229 1545 1115 250 202 182 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2737 1108 Size: 1629 3991 PfoI T'CCnGG_A Cuts at: 2411 2411 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2248 2962 1936 Size: 312 714 4594 Fragments arranged by size: 4594 714 312 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2395 2506 2869 2968 4103 4784 4975 Size: 219 1350 111 363 99 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1350 1135 681 363 219 191 111 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3724 4625 4887 820 Size: 197 926 1781 901 262 1553 Fragments arranged by size: 1781 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2594 2978 1132 Size: 1462 384 3774 Fragments arranged by size: 3774 1462 384 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2900 1517 Size: 1383 4237 StyI C'CwwG_G Cuts at: 513 1065 3059 513 Size: 552 1994 3074 Fragments arranged by size: 3074 1994 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3647 3664 3817 4002 4447 325 Size: 1255 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3866 5578 Size: 274 80 33 51 1443 204 1685 1712 Cuts at: 5578 96 Size: 138 Fragments arranged by size: 1712 1685 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI