Restriction map for
pFN21AE3836
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# NO CUT ENZYMES ( 32 enzymes) #
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AclI AscI AseI AsnI AvrII
BlnI BsiWI BssHII Bst1107I BstBI
BstZ17I DraI EcoRV HpaI MfeI
MluI MunI NotI NruI NspV
PacI PaeR7I PmeI PshBI Psp1406I
PvuI ScaI SfuI SnaBI SspI
SwaI XhoI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AatII 12286 GACGTC GACGT^C
Acc65I 9470 GGTACC G^GTACC
AflII 9154 CTTAAG C^TTAAG
AocI 13603 CCTGAGG CC^T[ACGT]AGG
Asp718I 9470 GGTACC G^GTACC
BclI 5406 TGATCA T^GATCA
BfrI 9154 CTTAAG C^TTAAG
BglII 3959 AGATCT A^GATCT
BsmI 11677 GCATTC GAATGC(1/-1)
Bsu36I 13603 CCTGAGG CC^T[ACGT]AGG
Eco81I 13603 CCTGAGG CC^T[ACGT]AGG
EcoT22I 3222 ATGCAT ATGCA^T
FbaI 5406 TGATCA T^GATCA
FseI 1597 GGCCGGCC GGCCGG^CC
HindIII 12193 AAGCTT A^AGCTT
KpnI 9470 GGTACC GGTAC^C
NdeI 513 CATATG CA^TATG
NheI 10006 GCTAGC G^CTAGC
NsiI 3222 ATGCAT ATGCA^T
SalI 1084 GTCGAC G^TCGAC
SgrAI 11821 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3997 ACTAGT A^CTAGT
XbaI 6018 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 4130 TCCGGA T^CCGGA
5636 TCCGGA
AgeI 2714 ACCGGT A^CCGGT
9104 ACCGGT
AhdI 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4354 GACGCCAGGTC
Alw44I 4276 GTGCAC G^TGCAC
5616 GTGCAC
ApaLI 4276 GTGCAC G^TGCAC
5616 GTGCAC
AspEI 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4354 GACGCCAGGTC
BseAI 4130 TCCGGA T^CCGGA
5636 TCCGGA
Bsp1407I 8438 TGTACA T^GTACA
11609 TGTACA
BspEI 4130 TCCGGA T^CCGGA
5636 TCCGGA
BsrGI 8438 TGTACA T^GTACA
11609 TGTACA
CpoI 2690 CGGACCG CG^G[AT]CCG
4675 CGGACCG
Eam1105I 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4354 GACGCCAGGTC
EcoNI 3598 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
9714 CCTGTTTGAGG
EcoRI 5708 GAATTC G^AATTC
7560 GAATTC
KspI 3966 CCGCGG CCGC^GG
9039 CCGCGG
MroI 4130 TCCGGA T^CCGGA
5636 TCCGGA
PinAI 2714 ACCGGT A^CCGGT
9104 ACCGGT
RsrII 2690 CGGACCG CG^G[AT]CCG
4675 CGGACCG
SacII 3966 CCGCGG CCGC^GG
9039 CCGCGG
SbfI 3431 CCTGCAGG CCTGCA^GG
4572 CCTGCAGG
SfiI 5218 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5227 GGCCCCGTGGGCC
SphI 779 GCATGC GCATG^C
12755 GCATGC
Sse8387I 3431 CCTGCAGG CCTGCA^GG
4572 CCTGCAGG
SspBI 8438 TGTACA T^GTACA
11609 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 179 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI