Restriction map for
pFN21AE3836
(Linear) (Six-base) MAPSORT of: KIEE3836.seq Check: 6523 from: 1 to: 13632
KIEE3836.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 403 484 2689 2812 2932 3667 4566 4738
Size: 403 81 2205 123 120 735 899 172
Cuts at: 4738 7555 11506 11584 11799 12798 12850 13093 13632
Size: 2817 3951 78 215 999 52 243 539
Fragments arranged by size:
3951 2817 2205 999 899 735 539 403
243 215 172 123 120 81 78 52
AatII G_ACGT'C
Cuts at: 0 12290 13632
Size: 12290 1342
AccI GT'mk_AC
Cuts at: 0 584 658 1085 1142 2294 4052 4472 9731
Size: 584 74 427 57 1152 1758 420 5259
Cuts at: 9731 13632
Size: 3901
Fragments arranged by size:
5259 3901 1758 1152 584 427 420 74
57
Acc65I G'GTAC_C
Cuts at: 0 9470 13632
Size: 9470 4162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 687 1512 1810 5196 6584 6753 7602
Size: 406 281 825 298 3386 1388 169 849
Cuts at: 7602 8938 11890 12261 13221 13632
Size: 1336 2952 371 960 411
Fragments arranged by size:
3386 2952 1388 1336 960 849 825 411
406 371 298 281 169
AfeI AGC'GCT
Cuts at: 0 2608 8371 8983 11257 13632
Size: 2608 5763 612 2274 2375
Fragments arranged by size:
5763 2608 2375 2274 612
AflII C'TTAA_G
Cuts at: 0 9154 13632
Size: 9154 4478
AflIII A'CryG_T
Cuts at: 0 937 2860 6290 6470 8140 8354 8935 9146
Size: 937 1923 3430 180 1670 214 581 211
Cuts at: 9146 11285 11912 13632
Size: 2139 627 1720
Fragments arranged by size:
3430 2139 1923 1720 1670 937 627 581
214 211 180
AgeI A'CCGG_T
Cuts at: 0 2714 9104 13632
Size: 2714 6390 4528
Fragments arranged by size:
6390 4528 2714
AhdI GACnn_n'nnGTC
Cuts at: 0 1548 4359 13632
Size: 1548 2811 9273
Fragments arranged by size:
9273 2811 1548
AleI CACnn'nnGTG
Cuts at: 0 368 1363 2556 3452 4942 5485 6469 6832
Size: 368 995 1193 896 1490 543 984 363
Cuts at: 6832 8353 8519 9941 9988 10948 12544 13632
Size: 1521 166 1422 47 960 1596 1088
Fragments arranged by size:
1596 1521 1490 1422 1193 1088 995 984
960 896 543 368 363 166 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3247 3470 4460 8448 8784 9719 9959 10897
Size: 3247 223 990 3988 336 935 240 938
Cuts at: 10897 10966 12562 12830 13195 13632
Size: 69 1596 268 365 437
Fragments arranged by size:
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336 268 240 223 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3406 3438 13632
Size: 3406 32 10194
Fragments arranged by size:
10194 3406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2594 5100 6304 6504 7255 7473 7868 8253
Size: 2594 2506 1204 200 751 218 395 385
Cuts at: 8253 8730 8852 9165 11372 11607 12842 13037 13632
Size: 477 122 313 2207 235 1235 195 595
Fragments arranged by size:
2594 2506 2207 1235 1204 751 595 477
395 385 313 235 218 200 195 122
ApaI G_GGCC'C
Cuts at: 0 317 392 651 1868 3647 4642 5230 12793
Size: 317 75 259 1217 1779 995 588 7563
Cuts at: 12793 13217 13603 13632
Size: 424 386 29
Fragments arranged by size:
7563 1779 1217 995 588 424 386 317
259 75 29
ApaLI G'TGCA_C
Cuts at: 0 4276 5616 13632
Size: 4276 1340 8016
Fragments arranged by size:
8016 4276 1340
ApoI r'AATT_y
Cuts at: 0 5708 7560 9640 13632
Size: 5708 1852 2080 3992
Fragments arranged by size:
5708 3992 2080 1852
AvaI C'yCGr_G
Cuts at: 0 1361 1412 1682 3085 4036 4187 5451 6029
Size: 1361 51 270 1403 951 151 1264 578
Cuts at: 6029 7089 8365 8551 9925 10345 10403 11059 11430
Size: 1060 1276 186 1374 420 58 656 371
Cuts at: 11430 11928 11988 13632
Size: 498 60 1644
Fragments arranged by size:
1644 1403 1374 1361 1276 1264 1060 951
656 578 498 420 371 270 186 151
60 58 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7113 7146 13632
Size: 7113 33 6486
Fragments arranged by size:
7113 6486 33
BamHI G'GATC_C
Cuts at: 0 1965 4182 4196 5718 13632
Size: 1965 2217 14 1522 7914
Fragments arranged by size:
7914 2217 1965 1522 14
BanI G'GyrC_C
Cuts at: 0 472 1378 2132 2407 3594 3876 4265 4830
Size: 472 906 754 275 1187 282 389 565
Cuts at: 4830 5536 6463 6494 6736 6748 8023 8347 9470
Size: 706 927 31 242 12 1275 324 1123
Cuts at: 9470 9784 11138 11494 11764 12730 12770 13632
Size: 314 1354 356 270 966 40 862
Fragments arranged by size:
1354 1275 1187 1123 966 927 906 862
754 706 565 472 389 356 324 314
282 275 270 242 40 31 12
BanII G_rGCy'C
Cuts at: 0 317 332 392 651 1689 1868 3542 3647
Size: 317 15 60 259 1038 179 1674 105
Cuts at: 3647 3993 4316 4642 5230 5458 6850 8617 9440
Size: 346 323 326 588 228 1392 1767 823
Cuts at: 9440 9447 9771 10097 10457 10580 11198 12008 12490
Size: 7 324 326 360 123 618 810 482
Cuts at: 12490 12793 13217 13603 13632
Size: 303 424 386 29
Fragments arranged by size:
1767 1674 1392 1038 823 810 618 588
482 424 386 360 346 326 326 324
323 317 303 259 228 179 123 105
60 29 15 7
BbeI G_GCGC'C
Cuts at: 0 2136 4269 8027 11142 13632
Size: 2136 2133 3758 3115 2490
Fragments arranged by size:
3758 3115 2490 2136 2133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1312 5876 6432 6572 7708 9831 11569 12327
Size: 1312 4564 556 140 1136 2123 1738 758
Cuts at: 12327 13227 13632
Size: 900 405
Fragments arranged by size:
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405 140
BbvCI CC'TCA_GC
Cuts at: 0 2489 3916 8756 9301 13632
Size: 2489 1427 4840 545 4331
Fragments arranged by size:
4840 4331 2489 1427 545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1062 1096 2684 2718 3879 3913 7141 7175
Size: 1062 34 1588 34 1161 34 3228 34
Cuts at: 7175 9528 9562 11050 11084 11271 11305 11874 11908
Size: 2353 34 1488 34 187 34 569 34
Cuts at: 11908 13632
Size: 1724
Fragments arranged by size:
3228 2353 1724 1588 1488 1161 1062 569
187 34 34 34 34 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 526 6098 6643 13632
Size: 526 5572 545 6989
Fragments arranged by size:
6989 5572 545 526
BclI T'GATC_A
Cuts at: 0 5406 13632
Size: 5406 8226
BfrBI ATG'CAT
Cuts at: 0 3224 13632
Size: 3224 10408
BglI GCCn_nnn'nGGC
Cuts at: 0 794 1523 1604 2106 2567 3629 4311 5225
Size: 794 729 81 502 461 1062 682 914
Cuts at: 5225 5234 5357 7520 9518 9656 11432 12779 12887
Size: 9 123 2163 1998 138 1776 1347 108
Cuts at: 12887 13632
Size: 745
Fragments arranged by size:
2163 1998 1776 1347 1062 914 794 745
729 682 502 461 138 123 108 81
9
BglII A'GATC_T
Cuts at: 0 3959 13632
Size: 3959 9673
BlpI GC'TnA_GC
Cuts at: 0 2110 9679 12484 13632
Size: 2110 7569 2805 1148
Fragments arranged by size:
7569 2805 2110 1148
Bme1580I G_kGCm'C
Cuts at: 0 317 392 651 777 1383 1868 3647 3881
Size: 317 75 259 126 606 485 1779 234
Cuts at: 3881 4280 4642 4835 5230 5431 5539 5620 6398
Size: 399 362 193 395 201 108 81 778
Cuts at: 6398 6549 6751 9869 9938 10649 11073 11086 11450
Size: 151 202 3118 69 711 424 13 364
Cuts at: 11450 11767 12557 12733 12775 12793 13198 13217 13603
Size: 317 790 176 42 18 405 19 386
Cuts at: 13603 13632
Size: 29
Fragments arranged by size:
3118 1779 790 778 711 606 485 424
405 399 395 386 364 362 317 317
259 234 202 201 193 176 151 126
108 81 75 69 42 29 19 18
13
BmgBI CAC'GTC
Cuts at: 0 2861 3306 3945 4765 4899 5591 5861 5874
Size: 2861 445 639 820 134 692 270 13
Cuts at: 5874 8141 8318 8936 9864 10233 12405 13233 13632
Size: 2267 177 618 928 369 2172 828 399
Fragments arranged by size:
2861 2267 2172 928 828 820 692 639
618 445 399 369 270 177 134 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 1155 2007 2151 2550 3048 3311 4086 4224
Size: 1155 852 144 399 498 263 775 138
Cuts at: 4224 5007 5743 5820 7317 8205 8573 9339 9498
Size: 783 736 77 1497 888 368 766 159
Cuts at: 9498 9621 9753 10238 11907 12066 12189 12485 12864
Size: 123 132 485 1669 159 123 296 379
Cuts at: 12864 13632
Size: 768
Fragments arranged by size:
1669 1497 1155 888 852 783 775 768
766 736 498 485 399 379 368 296
263 159 159 144 138 132 123 123
77
BmtI G_CTAG'C
Cuts at: 0 10010 13632
Size: 10010 3622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3417 4263 4308 7914 8004 13632
Size: 3417 846 45 3606 90 5628
Fragments arranged by size:
5628 3606 3417 846 90 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 2056 3000 3255 4351 5122 5347 6262
Size: 1915 141 944 255 1096 771 225 915
Cuts at: 6262 6348 6801 7007 7516 7845 13632
Size: 86 453 206 509 329 5787
Fragments arranged by size:
5787 1915 1096 944 915 771 509 453
329 255 225 206 141 86
Bpu10I CC'TnA_GC
Cuts at: 0 2219 2489 3916 4505 5827 6689 8756 9301
Size: 2219 270 1427 589 1322 862 2067 545
Cuts at: 9301 12388 13352 13632
Size: 3087 964 280
Fragments arranged by size:
3087 2219 2067 1427 1322 964 862 589
545 280 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 6610 8860 13632
Size: 751 5859 2250 4772
Fragments arranged by size:
5859 4772 2250 751
BsaI GGTCTCn'nnnn_
Cuts at: 0 712 1699 1774 4279 5007 6110 6961 8173
Size: 712 987 75 2505 728 1103 851 1212
Cuts at: 8173 10781 13632
Size: 2608 2851
Fragments arranged by size:
2851 2608 2505 1212 1103 987 851 728
712 75
BsaAI yAC'GTr
Cuts at: 0 635 843 2544 6000 7344 8355 12764 13632
Size: 635 208 1701 3456 1344 1011 4409 868
Fragments arranged by size:
4409 3456 1701 1344 1011 868 635 208
BsaBI GATnn'nnATC
Cuts at: 0 856 1157 4774 13632
Size: 856 301 3617 8858
Fragments arranged by size:
8858 3617 856 301
BsaHI Gr'CG_yC
Cuts at: 0 65 1404 2133 2516 4266 4355 6416 7184
Size: 65 1339 729 383 1750 89 2061 768
Cuts at: 7184 7637 7895 8024 9620 10904 11139 12188 12287
Size: 453 258 129 1596 1284 235 1049 99
Cuts at: 12287 13632
Size: 1345
Fragments arranged by size:
2061 1750 1596 1345 1339 1284 1049 768
729 453 383 258 235 129 99 89
65
BsaWI w'CCGG_w
Cuts at: 0 2195 2714 4130 4673 5636 9104 13632
Size: 2195 519 1416 543 963 3468 4528
Fragments arranged by size:
4528 3468 2195 1416 963 543 519
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2029 2059 2274 2304 2806 2836 4573 4603
Size: 2029 30 215 30 502 30 1737 30
Cuts at: 4603 4804 4834 4987 5017 6043 6073 6799 6829
Size: 201 30 153 30 1026 30 726 30
Cuts at: 6829 6832 6862 7085 7115 7669 7699 11227 11257
Size: 3 30 223 30 554 30 3528 30
Cuts at: 11257 13632
Size: 2375
Fragments arranged by size:
3528 2375 2029 1737 1026 726 554 502
223 215 201 153 30 30 30 30
30 30 30 30 30 30 30 30
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1434 2017 3424 8319 9790 10945 11077 11256
Size: 1434 583 1407 4895 1471 1155 132 179
Cuts at: 11256 11259 11332 13240 13632
Size: 3 73 1908 392
Fragments arranged by size:
4895 1908 1471 1434 1407 1155 583 392
179 132 73 3
BseYI C'CCAG_C
Cuts at: 0 206 728 1298 2128 2390 2658 3639 3752
Size: 206 522 570 830 262 268 981 113
Cuts at: 3752 4261 4559 5355 5573 6878 7066 7382 8005
Size: 509 298 796 218 1305 188 316 623
Cuts at: 8005 8009 10524 11504 12707 12803 13114 13632
Size: 4 2515 980 1203 96 311 518
Fragments arranged by size:
2515 1305 1203 981 980 830 796 623
570 522 518 509 316 311 298 268
262 218 206 188 113 96 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 3135 5113 6691 7345 7737 8429 8549
Size: 820 2315 1978 1578 654 392 692 120
Cuts at: 8549 8673 8754 8976 9298 9481 9799 9984 10140
Size: 124 81 222 322 183 318 185 156
Cuts at: 10140 10449 10692 10809 12139 12489 12828 12972 13050
Size: 309 243 117 1330 350 339 144 78
Cuts at: 13050 13077 13632
Size: 27 555
Fragments arranged by size:
2315 1978 1578 1330 820 692 654 555
392 350 339 322 318 309 243 222
185 183 156 144 124 120 117 81
78 27
BsiEI CG_ry'CG
Cuts at: 0 2163 8460 8520 8931 9513 10422 10653 10755
Size: 2163 6297 60 411 582 909 231 102
Cuts at: 10755 12903 13632
Size: 2148 729
Fragments arranged by size:
6297 2163 2148 909 729 582 411 231
102 60
BsiHKAI G_wGCw'C
Cuts at: 0 332 463 679 812 1235 1652 3092 3542
Size: 332 131 216 133 423 417 1440 450
Cuts at: 3542 3556 3739 4163 4280 5620 5833 6695 7292
Size: 14 183 424 117 1340 213 862 597
Cuts at: 7292 7578 7967 8617 8705 10144 10381 12716 12925
Size: 286 389 650 88 1439 237 2335 209
Cuts at: 12925 13232 13632
Size: 307 400
Fragments arranged by size:
2335 1440 1439 1340 862 650 597 450
424 423 417 400 389 332 307 286
237 216 213 209 183 133 131 117
88 14
BsmI GAATG_Cn'
Cuts at: 0 11677 13632
Size: 11677 1955
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2164 4962 5590 12073 13632
Size: 2164 2798 628 6483 1559
Fragments arranged by size:
6483 2798 2164 1559 628
Bsp1286I G_dGCh'C
Cuts at: 0 317 332 392 463 651 679 777 812
Size: 317 15 60 71 188 28 98 35
Cuts at: 812 1235 1383 1652 1689 1868 3092 3542 3556
Size: 423 148 269 37 179 1224 450 14
Cuts at: 3556 3647 3739 3881 3993 4163 4280 4316 4642
Size: 91 92 142 112 170 117 36 326
Cuts at: 4642 4835 5230 5431 5458 5539 5620 5833 6398
Size: 193 395 201 27 81 81 213 565
Cuts at: 6398 6549 6695 6751 6850 7292 7578 7967 8617
Size: 151 146 56 99 442 286 389 650
Cuts at: 8617 8705 9440 9447 9771 9869 9938 10097 10144
Size: 88 735 7 324 98 69 159 47
Cuts at: 10144 10381 10457 10580 10649 11073 11086 11198 11450
Size: 237 76 123 69 424 13 112 252
Cuts at: 11450 11767 12008 12490 12557 12716 12733 12775 12793
Size: 317 241 482 67 159 17 42 18
Cuts at: 12793 12925 13198 13217 13232 13603 13632
Size: 132 273 19 15 371 29
Fragments arranged by size:
1224 735 650 565 482 450 442 424
423 395 389 371 326 324 317 317
286 273 269 252 241 237 213 201
193 188 179 170 159 159 151 148
146 142 132 123 117 112 112 99
98 98 92 91 88 81 81 76
71 69 69 67 60 56 47 42
37 36 35 29 28 27 19 18
17 15 15 14 13 7
BspEI T'CCGG_A
Cuts at: 0 4130 5636 13632
Size: 4130 1506 7996
Fragments arranged by size:
7996 4130 1506
BspHI T'CATG_A
Cuts at: 0 1673 4703 11558 13632
Size: 1673 3030 6855 2074
Fragments arranged by size:
6855 3030 2074 1673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 403 448 484 1516 2689 2812 2932 2956
Size: 403 45 36 1032 1173 123 120 24
Cuts at: 2956 3106 3667 4566 4580 4738 6608 7555 7636
Size: 150 561 899 14 158 1870 947 81
Cuts at: 7636 7855 8578 9122 9270 9839 10363 10966 11506
Size: 219 723 544 148 569 524 603 540
Cuts at: 11506 11584 11799 12611 12798 12850 13093 13135 13632
Size: 78 215 812 187 52 243 42 497
Fragments arranged by size:
1870 1173 1032 947 899 812 723 603
569 561 544 540 524 497 403 243
219 215 187 158 150 148 123 120
81 78 52 45 42 36 24 14
BsrBI CCG'CTC
Cuts at: 0 557 1776 2355 2891 5606 6102 7206 7555
Size: 557 1219 579 536 2715 496 1104 349
Cuts at: 7555 7983 10865 11429 12171 13632
Size: 428 2882 564 742 1461
Fragments arranged by size:
2882 2715 1461 1219 1104 742 579 564
557 536 496 428 349
BsrDI GCAATG_nn'
Cuts at: 0 282 946 980 5906 7728 8718 9279 10305
Size: 282 664 34 4926 1822 990 561 1026
Cuts at: 10305 12729 13152 13632
Size: 2424 423 480
Fragments arranged by size:
4926 2424 1822 1026 990 664 561 480
423 282 34
BsrFI r'CCGG_y
Cuts at: 0 587 1598 1708 2714 3025 3206 4147 4288
Size: 587 1011 110 1006 311 181 941 141
Cuts at: 4288 4522 5623 6406 6452 6592 9104 9818 10121
Size: 234 1101 783 46 140 2512 714 303
Cuts at: 10121 11093 11822 12767 12914 13632
Size: 972 729 945 147 718
Fragments arranged by size:
2512 1101 1011 1006 972 945 941 783
729 718 714 587 311 303 234 181
147 141 140 110 46
BsrGI T'GTAC_A
Cuts at: 0 8438 11609 13632
Size: 8438 3171 2023
Fragments arranged by size:
8438 3171 2023
BssSI C'ACGA_G
Cuts at: 0 1582 3165 4423 13632
Size: 1582 1583 1258 9209
Fragments arranged by size:
9209 1583 1582 1258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 440 2450 8318 9911 12464 13632
Size: 440 2010 5868 1593 2553 1168
Fragments arranged by size:
5868 2553 2010 1593 1168 440
BstEII G'GTnAC_C
Cuts at: 0 5877 9493 10350 13632
Size: 5877 3616 857 3282
Fragments arranged by size:
5877 3616 3282 857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 2236 3903 4143 5373 7143 9447 10532
Size: 696 1540 1667 240 1230 1770 2304 1085
Cuts at: 10532 11149 12021 12147 12352 13395 13632
Size: 617 872 126 205 1043 237
Fragments arranged by size:
2304 1770 1667 1540 1230 1085 1043 872
696 617 240 237 205 126
BstYI r'GATC_y
Cuts at: 0 166 908 1031 1965 2372 3665 3959 4182
Size: 166 742 123 934 407 1293 294 223
Cuts at: 4182 4196 4775 5718 7790 11630 12341 13632
Size: 14 579 943 2072 3840 711 1291
Fragments arranged by size:
3840 2072 1293 1291 943 934 742 711
579 407 294 223 166 123 14
Bsu36I CC'TnA_GG
Cuts at: 0 13604 13632
Size: 13604 28
BtgI C'CryG_G
Cuts at: 0 695 998 1823 2250 3966 4319 4538 4601
Size: 695 303 825 427 1716 353 219 63
Cuts at: 4601 4980 5112 5231 5459 6522 6549 6851 6873
Size: 379 132 119 228 1063 27 302 22
Cuts at: 6873 7000 7391 7406 8997 9039 9824 12152 12776
Size: 127 391 15 1591 42 785 2328 624
Cuts at: 12776 13542 13632
Size: 766 90
Fragments arranged by size:
2328 1716 1591 1063 825 785 766 695
624 427 391 379 353 303 302 228
219 132 127 119 90 63 42 27
22 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 3116 3260 3290 3882 6221 6693 7386
Size: 225 2891 144 30 592 2339 472 693
Cuts at: 7386 8215 8282 8406 9989 10193 10746 10796 11033
Size: 829 67 124 1583 204 553 50 237
Cuts at: 11033 11162 11598 11871 12103 13632
Size: 129 436 273 232 1529
Fragments arranged by size:
2891 2339 1583 1529 829 693 592 553
472 436 273 237 232 225 204 144
129 124 67 50 30
BtsI GCAGTG_nn'
Cuts at: 0 318 1545 2295 2355 4454 5093 5556 5918
Size: 318 1227 750 60 2099 639 463 362
Cuts at: 5918 7328 7644 7720 7923 8184 8254 8685 8745
Size: 1410 316 76 203 261 70 431 60
Cuts at: 8745 8761 10452 10666 10869 11217 12613 12925 12989
Size: 16 1691 214 203 348 1396 312 64
Cuts at: 12989 13137 13632
Size: 148 495
Fragments arranged by size:
2099 1691 1410 1396 1227 750 639 495
463 431 362 348 318 316 312 261
214 203 203 148 76 70 64 60
60 16
ClaI AT'CG_AT
Cuts at: 0 899 3845 5336 13632
Size: 899 2946 1491 8296
Fragments arranged by size:
8296 2946 1491 899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1865 1900 2739 2774 2985 3020 7440 7475
Size: 1865 35 839 35 211 35 4420 35
Cuts at: 7475 8016 8051 8829 8864 9876 9911 13053 13088
Size: 541 35 778 35 1012 35 3142 35
Cuts at: 13088 13632
Size: 544
Fragments arranged by size:
4420 3142 1865 1012 839 778 544 541
211 35 35 35 35 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 843 1809 6933 8505 9675 10994 11445 13632
Size: 843 966 5124 1572 1170 1319 451 2187
Fragments arranged by size:
5124 2187 1572 1319 1170 966 843 451
DrdI GACnn_nn'nnGTC
Cuts at: 0 940 2074 3868 6436 9835 10231 11695 11842
Size: 940 1134 1794 2568 3399 396 1464 147
Cuts at: 11842 13632
Size: 1790
Fragments arranged by size:
3399 2568 1794 1790 1464 1134 940 396
147
EaeI y'GGCC_r
Cuts at: 0 629 854 1484 1600 1749 1820 1886 2456
Size: 629 225 630 116 149 71 66 570
Cuts at: 2456 2835 4028 4109 4145 4398 4524 5177 5217
Size: 379 1193 81 36 253 126 653 40
Cuts at: 5217 5375 5643 5892 6482 7145 7409 8775 8928
Size: 158 268 249 590 663 264 1366 153
Cuts at: 8928 9510 10650 10752 10866 11451 11529 11649 11733
Size: 582 1140 102 114 585 78 120 84
Cuts at: 11733 11903 12149 12354 13101 13134 13310 13562 13632
Size: 170 246 205 747 33 176 252 70
Fragments arranged by size:
1366 1193 1140 747 663 653 630 629
590 585 582 570 379 268 264 253
252 249 246 225 205 176 170 158
153 149 126 120 116 114 102 84
81 78 71 70 66 40 36 33
EagI C'GGCC_G
Cuts at: 0 8928 9510 10650 10752 13632
Size: 8928 582 1140 102 2880
Fragments arranged by size:
8928 2880 1140 582 102
EarI CTCTTCn'nnn_
Cuts at: 0 2269 3277 5215 7972 10580 10694 11177 13632
Size: 2269 1008 1938 2757 2608 114 483 2455
Fragments arranged by size:
2757 2608 2455 2269 1938 1008 483 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7933 13448 13632
Size: 7933 5515 184
Fragments arranged by size:
7933 5515 184
EcoICRI GAG'CTC
Cuts at: 0 330 3540 8615 13632
Size: 330 3210 5075 5017
Fragments arranged by size:
5075 5017 3210 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 687 1512 1810 1915 2056 3000 3255
Size: 406 281 825 298 105 141 944 255
Cuts at: 3255 4351 5122 5196 5347 6262 6348 6584 6753
Size: 1096 771 74 151 915 86 236 169
Cuts at: 6753 6801 7007 7516 7602 7845 8938 11890 12261
Size: 48 206 509 86 243 1093 2952 371
Cuts at: 12261 13221 13632
Size: 960 411
Fragments arranged by size:
2952 1096 1093 960 944 915 825 771
509 411 406 371 298 281 255 243
236 206 169 151 141 105 86 86
74 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3602 9718 13632
Size: 3602 6116 3914
Fragments arranged by size:
6116 3914 3602
EcoO109I rG'GnC_Cy
Cuts at: 0 177 313 314 647 1638 2007 2215 2655
Size: 177 136 1 333 991 369 208 440
Cuts at: 2655 3170 3643 3756 4041 4124 4638 4639 5097
Size: 515 473 113 285 83 514 1 458
Cuts at: 5097 5226 5227 5759 6684 6953 7470 7844 8871
Size: 129 1 532 925 269 517 374 1027
Cuts at: 8871 9162 9282 9398 11604 12384 12790 13049 13213
Size: 291 120 116 2206 780 406 259 164
Cuts at: 13213 13214 13599 13600 13620 13632
Size: 1 385 1 20 12
Fragments arranged by size:
2206 1027 991 925 780 532 517 515
514 473 458 440 406 385 374 369
333 291 285 269 259 208 177 164
136 129 120 116 113 83 20 12
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 5708 7560 13632
Size: 5708 1852 6072
Fragments arranged by size:
6072 5708 1852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4941 7191 8901 13632
Size: 4941 2250 1710 4731
Fragments arranged by size:
4941 4731 2250 1710
FseI GG_CCGG'CC
Cuts at: 0 1602 13632
Size: 1602 12030
FspI TGC'GCA
Cuts at: 0 2347 3889 9118 11398 13632
Size: 2347 1542 5229 2280 2234
Fragments arranged by size:
5229 2347 2280 2234 1542
FspAI rTGC'GCAy
Cuts at: 0 11398 13632
Size: 11398 2234
HaeII r_GCGC'y
Cuts at: 0 2136 2610 3243 3929 4269 7087 7731 8027
Size: 2136 474 633 686 340 2818 644 296
Cuts at: 8027 8121 8373 8607 8748 8985 10773 10893 11142
Size: 94 252 234 141 237 1788 120 249
Cuts at: 11142 11259 13632
Size: 117 2373
Fragments arranged by size:
2818 2373 2136 1788 686 644 633 474
340 296 252 249 237 234 141 120
117 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 541 573 1534 1566 2262 2294
Size: 445 32 64 32 961 32 696 32
Cuts at: 2294 2850 2882 3209 3241 3538 3570 4345 4377
Size: 556 32 327 32 297 32 775 32
Cuts at: 4377 4417 4449 4987 5019 5398 5430 6559 6591
Size: 40 32 538 32 379 32 1129 32
Cuts at: 6591 6799 6831 7085 7117 7657 7689 13632
Size: 208 32 254 32 540 32 5943
Fragments arranged by size:
5943 1129 961 775 696 556 540 538
445 379 327 297 254 208 64 40
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1086 5556 6141 7503 9888 12372 12852 13062
Size: 1086 4470 585 1362 2385 2484 480 210
Cuts at: 13062 13632
Size: 570
Fragments arranged by size:
4470 2484 2385 1362 1086 585 570 480
210
HindIII A'AGCT_T
Cuts at: 0 12193 13632
Size: 12193 1439
KasI G'GCGC_C
Cuts at: 0 2132 4265 8023 11138 13632
Size: 2132 2133 3758 3115 2494
Fragments arranged by size:
3758 3115 2494 2133 2132
KpnI G_GTAC'C
Cuts at: 0 9474 13632
Size: 9474 4158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2483 3845 5396 6623 6819 7763 9164 10019
Size: 2483 1362 1551 1227 196 944 1401 855
Cuts at: 10019 10513 11837 12586 13632
Size: 494 1324 749 1046
Fragments arranged by size:
2483 1551 1401 1362 1324 1227 1046 944
855 749 494 196
MscI TGG'CCA
Cuts at: 0 631 856 1486 1888 2458 4030 4111 4400
Size: 631 225 630 402 570 1572 81 289
Cuts at: 4400 5179 5219 5377 5645 6484 7147 7411 12356
Size: 779 40 158 268 839 663 264 4945
Cuts at: 12356 13564 13632
Size: 1208 68
Fragments arranged by size:
4945 1572 1208 839 779 663 631 630
570 402 289 268 264 225 158 81
68 40
MslI CAynn'nnrTG
Cuts at: 0 368 694 1363 1430 1624 2556 3191 3452
Size: 368 326 669 67 194 932 635 261
Cuts at: 3452 4408 4942 5173 5485 5911 6172 6289 6469
Size: 956 534 231 312 426 261 117 180
Cuts at: 6469 6832 6979 7232 7405 8128 8353 8519 8822
Size: 363 147 253 173 723 225 166 303
Cuts at: 8822 9379 9520 9941 9988 10471 10948 12019 12544
Size: 557 141 421 47 483 477 1071 525
Cuts at: 12544 13393 13632
Size: 849 239
Fragments arranged by size:
1071 956 932 849 723 669 635 557
534 525 483 477 426 421 368 363
326 312 303 261 261 253 239 231
225 194 180 173 166 147 141 117
67 47
MspA1I CmG'CkG
Cuts at: 0 1514 2594 2732 2840 3071 3968 5303 5355
Size: 1514 1080 138 108 231 897 1335 52
Cuts at: 5355 5523 6155 6504 6531 7329 7427 7694 7832
Size: 168 632 349 27 798 98 267 138
Cuts at: 7832 7868 8009 8428 8591 8897 9041 9059 10274
Size: 36 141 419 163 306 144 18 1215
Cuts at: 10274 10295 10499 10517 10625 10640 10655 10757 10835
Size: 21 204 18 108 15 15 102 78
Cuts at: 10835 11004 11351 11543 12626 12662 12905 12986 13037
Size: 169 347 192 1083 36 243 81 51
Cuts at: 13037 13118 13632
Size: 81 514
Fragments arranged by size:
1514 1335 1215 1083 1080 897 798 632
514 419 349 347 306 267 243 231
204 192 169 168 163 144 141 138
138 108 108 102 98 81 81 78
52 51 36 36 27 21 18 18
15 15
NaeI GCC'GGC
Cuts at: 0 1600 3027 3208 5625 6594 10123 13632
Size: 1600 1427 181 2417 969 3529 3509
Fragments arranged by size:
3529 3509 2417 1600 1427 969 181
NarI GG'CG_CC
Cuts at: 0 2133 4266 8024 11139 13632
Size: 2133 2133 3758 3115 2493
Fragments arranged by size:
3758 3115 2493 2133 2133
NcoI C'CATG_G
Cuts at: 0 695 998 2250 4601 5112 7391 8997 9824
Size: 695 303 1252 2351 511 2279 1606 827
Cuts at: 9824 13542 13632
Size: 3718 90
Fragments arranged by size:
3718 2351 2279 1606 1252 827 695 511
303 90
NdeI CA'TA_TG
Cuts at: 0 514 13632
Size: 514 13118
NgoMIV G'CCGG_C
Cuts at: 0 1598 3025 3206 5623 6592 10121 13632
Size: 1598 1427 181 2417 969 3529 3511
Fragments arranged by size:
3529 3511 2417 1598 1427 969 181
NheI G'CTAG_C
Cuts at: 0 10006 13632
Size: 10006 3626
NsiI A_TGCA'T
Cuts at: 0 3226 13632
Size: 3226 10406
NspI r_CATG'y
Cuts at: 0 739 783 941 5435 5616 6294 7746 9838
Size: 739 44 158 4494 181 678 1452 2092
Cuts at: 9838 11289 12545 12759 12953 13632
Size: 1451 1256 214 194 679
Fragments arranged by size:
4494 2092 1452 1451 1256 739 679 678
214 194 181 158 44
PasI CC'CwG_GG
Cuts at: 0 1949 2464 6012 6949 9234 9440 13632
Size: 1949 515 3548 937 2285 206 4192
Fragments arranged by size:
4192 3548 2285 1949 937 515 206
PciI A'CATG_T
Cuts at: 0 937 6290 11285 13632
Size: 937 5353 4995 2347
Fragments arranged by size:
5353 4995 2347 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 863 1809 1975 2502 2732 2855 4517 4602
Size: 863 946 166 527 230 123 1662 85
Cuts at: 4602 4986 5810 6933 7881 8135 8249 11897 12047
Size: 384 824 1123 948 254 114 3648 150
Cuts at: 12047 13560 13632
Size: 1513 72
Fragments arranged by size:
3648 1662 1513 1123 948 946 863 824
527 384 254 230 166 150 123 114
85 72
PfoI T'CCnGG_A
Cuts at: 0 284 1640 2600 2854 3758 5281 5755 6817
Size: 284 1356 960 254 904 1523 474 1062
Cuts at: 6817 7532 13632
Size: 715 6100
Fragments arranged by size:
6100 1523 1356 1062 960 904 715 474
284 254
PmlI CAC'GTG
Cuts at: 0 843 7344 8355 12764 13632
Size: 843 6501 1011 4409 868
Fragments arranged by size:
6501 4409 1011 868 843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10720 10752 13632
Size: 10720 32 2880
Fragments arranged by size:
10720 2880 32
PpuMI rG'GwC_Cy
Cuts at: 0 1638 2007 2215 3756 4124 5759 6684 7470
Size: 1638 369 208 1541 368 1635 925 786
Cuts at: 7470 7844 8871 9282 11604 13620 13632
Size: 374 1027 411 2322 2016 12
Fragments arranged by size:
2322 2016 1638 1635 1541 1027 925 786
411 374 369 368 208 12
PshAI GACnn'nnGTC
Cuts at: 0 3304 4954 7475 13129 13632
Size: 3304 1650 2521 5654 503
Fragments arranged by size:
5654 3304 2521 1650 503
PspOMI G'GGCC_C
Cuts at: 0 313 388 647 1864 3643 4638 5226 12789
Size: 313 75 259 1217 1779 995 588 7563
Cuts at: 12789 13213 13599 13632
Size: 424 386 33
Fragments arranged by size:
7563 1779 1217 995 588 424 386 313
259 75 33
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2524 2556 13632
Size: 2524 32 11076
Fragments arranged by size:
11076 2524 32
PstI C_TGCA'G
Cuts at: 0 103 481 545 2443 3034 3436 3577 4577
Size: 103 378 64 1898 591 402 141 1000
Cuts at: 4577 4965 5551 7918 7933 8857 8977 9904 11329
Size: 388 586 2367 15 924 120 927 1425
Cuts at: 11329 11542 12608 13144 13632
Size: 213 1066 536 488
Fragments arranged by size:
2367 1898 1425 1066 1000 927 924 591
586 536 488 402 388 378 213 141
120 103 64 15
PvuII CAG'CTG
Cuts at: 0 5355 5523 6155 6504 7427 8009 8428 9059
Size: 5355 168 632 349 923 582 419 631
Cuts at: 9059 11351 11543 12626 13118 13632
Size: 2292 192 1083 492 514
Fragments arranged by size:
5355 2292 1083 923 632 631 582 514
492 419 349 192 168
RsrII CG'GwC_CG
Cuts at: 0 2691 4676 13632
Size: 2691 1985 8956
Fragments arranged by size:
8956 2691 1985
SacI G_AGCT'C
Cuts at: 0 332 3542 8617 13632
Size: 332 3210 5075 5015
Fragments arranged by size:
5075 5015 3210 332
SacII CC_GC'GG
Cuts at: 0 3969 9042 13632
Size: 3969 5073 4590
Fragments arranged by size:
5073 4590 3969
SalI G'TCGA_C
Cuts at: 0 1084 13632
Size: 1084 12548
SanDI GG'GwC_CC
Cuts at: 0 4124 13620 13632
Size: 4124 9496 12
Fragments arranged by size:
9496 4124 12
SapI GCTCTTCn'nnn_
Cuts at: 0 3277 5215 7972 13632
Size: 3277 1938 2757 5660
Fragments arranged by size:
5660 3277 2757 1938
SbfI CC_TGCA'GG
Cuts at: 0 3436 4577 13632
Size: 3436 1141 9055
Fragments arranged by size:
9055 3436 1141
SexAI A'CCwGG_T
Cuts at: 0 1124 1349 2495 11600 13632
Size: 1124 225 1146 9105 2032
Fragments arranged by size:
9105 2032 1146 1124 225
SfcI C'TryA_G
Cuts at: 0 99 477 541 2439 3030 3126 3432 3573
Size: 99 378 64 1898 591 96 306 141
Cuts at: 3573 3864 4049 4573 4961 5547 5628 7914 7929
Size: 291 185 524 388 586 81 2286 15
Cuts at: 7929 8043 8853 8973 9900 11325 11538 12604 13140
Size: 114 810 120 927 1425 213 1066 536
Cuts at: 13140 13632
Size: 492
Fragments arranged by size:
2286 1898 1425 1066 927 810 591 586
536 524 492 388 378 306 291 213
185 141 120 114 99 96 81 64
15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5225 5234 13632
Size: 5225 9 8398
Fragments arranged by size:
8398 5225 9
SfoI GGC'GCC
Cuts at: 0 2134 4267 8025 11140 13632
Size: 2134 2133 3758 3115 2492
Fragments arranged by size:
3758 3115 2492 2134 2133
SgrAI Cr'CCGG_yG
Cuts at: 0 11822 13632
Size: 11822 1810
SmaI CCC'GGG
Cuts at: 0 1363 4189 6031 9927 10405 13632
Size: 1363 2826 1842 3896 478 3227
Fragments arranged by size:
3896 3227 2826 1842 1363 478
SmlI C'TyrA_G
Cuts at: 0 766 6625 8875 9154 13632
Size: 766 5859 2250 279 4478
Fragments arranged by size:
5859 4478 2250 766 279
SpeI A'CTAG_T
Cuts at: 0 3997 13632
Size: 3997 9635
SphI G_CATG'C
Cuts at: 0 783 12759 13632
Size: 783 11976 873
Fragments arranged by size:
11976 873 783
StuI AGG'CCT
Cuts at: 0 5653 6860 9299 10936 12146 12263 13457 13632
Size: 5653 1207 2439 1637 1210 117 1194 175
Fragments arranged by size:
5653 2439 1637 1210 1207 1194 175 117
StyI C'CwwG_G
Cuts at: 0 333 695 974 998 1634 2012 2250 4165
Size: 333 362 279 24 636 378 238 1915
Cuts at: 4165 4601 5112 7391 8997 9182 9824 10580 11522
Size: 436 511 2279 1606 185 642 756 942
Cuts at: 11522 12365 13344 13542 13625 13632
Size: 843 979 198 83 7
Fragments arranged by size:
2279 1915 1606 979 942 843 756 642
636 511 436 378 362 333 279 238
198 185 83 24 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 720 1967 2133 2708 3067 3357 3955 4068
Size: 720 1247 166 575 359 290 598 113
Cuts at: 4068 4178 4573 5170 5802 7013 7299 8001 8019
Size: 110 395 597 632 1211 286 702 18
Cuts at: 8019 8365 9010 9247 9321 9325 9754 10483 11230
Size: 346 645 237 74 4 429 729 747
Cuts at: 11230 11863 11889 11938 12817 13632
Size: 633 26 49 879 815
Fragments arranged by size:
1247 1211 879 815 747 729 720 702
645 633 632 598 597 575 429 395
359 346 290 286 237 166 113 110
74 49 26 18 4
TatI w'GTAC_w
Cuts at: 0 1847 1985 4886 5681 6197 8438 9347 9476
Size: 1847 138 2901 795 516 2241 909 129
Cuts at: 9476 11609 11915 12044 12176 13632
Size: 2133 306 129 132 1456
Fragments arranged by size:
2901 2241 2133 1847 1456 909 795 516
306 138 132 129 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1443 1475 2029 2061 2274 2306 5145 5177
Size: 1443 32 554 32 213 32 2839 32
Cuts at: 5177 5380 5412 5845 5877 11227 11259 13632
Size: 203 32 433 32 5350 32 2373
Fragments arranged by size:
5350 2839 2373 1443 554 433 213 203
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1126 2515 4680 4846 4999 7636 9592 9727
Size: 1126 1389 2165 166 153 2637 1956 135
Cuts at: 9727 11119 12406 13632
Size: 1392 1287 1226
Fragments arranged by size:
2637 2165 1956 1392 1389 1287 1226 1126
166 153 135
XbaI T'CTAG_A
Cuts at: 0 6018 13632
Size: 6018 7614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1762 1956 1976 5134 5811 6282 7341 8785
Size: 1762 194 20 3158 677 471 1059 1444
Cuts at: 8785 9447 9963 10477 11728 11898 13144 13530 13632
Size: 662 516 514 1251 170 1246 386 102
Fragments arranged by size:
3158 1762 1444 1251 1246 1059 677 662
516 514 471 386 194 170 102 20
XmaI C'CCGG_G
Cuts at: 0 1361 4187 6029 9925 10403 13632
Size: 1361 2826 1842 3896 478 3229
Fragments arranged by size:
3896 3229 2826 1842 1361 478
XmnI GAAnn'nnTTC
Cuts at: 0 3721 7705 8098 9086 13632
Size: 3721 3984 393 988 4546
Fragments arranged by size:
4546 3984 3721 988 393
ZraI GAC'GTC
Cuts at: 0 12288 13632
Size: 12288 1344
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AscI AseI AsiSI AvrII BsiWI BssHII BstBI
BstZ17I DraI EcoRV HpaI MfeI MluI NotI NruI
PacI PmeI PsiI PspXI PvuI ScaI SgfI SnaBI
SrfI SspI SwaI XhoI