Restriction map for
pFN21AE2498
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# NO CUT ENZYMES ( 36 enzymes) #
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AgeI AscI AviII BbrPI BsiWI
BssHII BstBI CpoI DrdI EagI
EclXI Eco52I FseI FspI MluI
NheI NotI NruI NspV PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SmaI Sse8387I SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 9766 GACGTC GACGT^C
AflII 12025 CTTAAG C^TTAAG
AhdI 8485 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 6050 AGCGCT AGC^GCT
AspEI 8485 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6342 CCTAGG C^CTAGG
BfrI 12025 CTTAAG C^TTAAG
BlnI 6342 CCTAGG C^CTAGG
BlpI 11920 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 11920 GCTGAGC GC^T[ACGT]AGC
BsiEI 3177 CGACCG CG[GA][CT]^CG
BspLU11I 2538 ACATGT A^CATGT
Bst1107I 1464 GTATAC GTA^TAC
BstEII 12966 GGTGACC G^GT[ACGT]ACC
BstPI 12966 GGTGACC G^GT[ACGT]ACC
BstZ17I 1464 GTATAC GTA^TAC
CelII 11920 GCTGAGC GC^T[ACGT]AGC
Eam1105I 8485 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6050 AGCGCT AGC^GCT
EcoNI 12171 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 12966 GGTGACC G^GT[ACGT]ACC
EcoT22I 7667 ATGCAT ATGCA^T
HpaI 5416 GTTAAC GTT^AAC
KspI 6741 CCGCGG CCGC^GG
NsiI 7667 ATGCAT ATGCA^T
PacI 11521 TTAATTAA TTAAT^TAA
SacI 10864 GAGCTC GAGCT^C
SacII 6741 CCGCGG CCGC^GG
SexAI 11310 ACCTGGT A^CC[AT]GGT
SnaBI 10503 TACGTA TAC^GTA
SpeI 3190 ACTAGT A^CTAGT
SphI 10037 GCATGC GCATG^C
XbaI 12087 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 1460 ACACGT A^C[GA][CT]GT
2538 ACATGT
AspI 6442 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
8962 GACAAAGTC
BbeI 6002 GGCGCC GGCGC^C
12595 GGCGCC
BbvCI 11009 CCTCAGC CCTCAGC(-5/-2)
12171 CCTCAGC
BspHI 1176 TCATGA T^CATGA
4565 TCATGA
BsrBI 11019 CCGCTC CCGCTC(-3/-3)
12391 GAGCGG
BstAPI 5004 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5922 GCAGTATGTGC
KasI 6002 GGCGCC G^GCGCC
12595 GGCGCC
NaeI 8648 GCCGGC GCC^GGC
12442 GCCGGC
NarI 6002 GGCGCC GG^CGCC
12595 GGCGCC
NgoMIV 8648 GCCGGC G^CCGGC
12442 GCCGGC
PaeR7I 1582 CTCGAG C^TCGAG
5072 CTCGAG
PflFI 6442 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
8962 GACAAAGTC
RcaI 1176 TCATGA T^CATGA
4565 TCATGA
SapI 8519 GCTCTTC GCTCTTC(1/4)
12759 GCTCTTC
ScaI 3292 AGTACT AGT^ACT
3981 AGTACT
SfoI 6002 GGCGCC GGC^GCC
12595 GGCGCC
Tth111I 6442 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
8962 GACAAAGTC
XhoI 1582 CTCGAG C^TCGAG
5072 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 170 enzymes) #
#########################################
Acc65I AccI AccII AccIII AciI
AclI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspMI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI Psp1406I
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI